常用小型實(shí)驗(yàn)動(dòng)物灌胃給藥技術(shù)方法（Techniques for intragastric administration of small experimental animals）

常用小型實(shí)驗(yàn)動(dòng)物灌胃給藥技術(shù)方法（Techniquesforintragastricadministrationofsmallexperimentalanimals）1頁(yè)維普資訊瀘州醫(yī)學(xué)院學(xué)報(bào)2oo4年第27卷第3期ofhizi.dumedicalcollegevd.27NO3硎[J].4結(jié)果5操作體會(huì)經(jīng)上述方法所制成的標(biāo)本如附圖所示。該標(biāo)本顯示結(jié)構(gòu)較多，內(nèi)容復(fù)雜，要求操作者對(duì)該部分的理論非常熟悉在去除小腦中腳時(shí)，最好。邊切邊拉切口的深度與剝離的進(jìn)程同步，將顯示內(nèi)。容全部暴露后，最后再用解剖刀修潔整齊；并注意保留三叉神經(jīng)根和前庭蝸神經(jīng)根，以便觀察毗鄰關(guān)系。修潔小腦下腳時(shí)，應(yīng)該用解剖鑷撕去部分纖維，使其與周邊關(guān)系呈自然移行狀。參考文獻(xiàn)1。李忠華。王興海主編。解剖學(xué)技術(shù)【圳第暖北京2。人民衛(wèi)生出版掛。1997；57～印2。張朝佑主編。人體解剖學(xué)M【】。第版北京人民衛(wèi)生2。出版社。1998；1215-1200附圖：經(jīng)剝制后的標(biāo)本（~0os-l】一19收稿）常用小型實(shí)驗(yàn)動(dòng)物灌胃給藥技術(shù)方法介紹趙明德（啻驀州『醫(yī)學(xué)院實(shí)驗(yàn)動(dòng)物科，四川瀘州64~000）關(guān)鍵詞小型動(dòng)物我灌胃；培藥方法中匿分類(lèi)號(hào)Q33文獻(xiàn)標(biāo)識(shí)碼B文章編號(hào)1000-2669（0o4）3-0258-02隨著生命科學(xué)技術(shù)的發(fā)展，動(dòng)物實(shí)驗(yàn)已成為醫(yī)小鼠、大鼠灌胃器的來(lái)源有兩個(gè)，一是從市面上學(xué)實(shí)驗(yàn)研究的基本方法。根據(jù)實(shí)驗(yàn)研究目的、實(shí)驗(yàn)購(gòu)買(mǎi)特制的I2一L6號(hào)灌胃針。二是自制。自制方法動(dòng)物的種類(lèi)及藥物劑型、荊量的不同，要求對(duì)實(shí)驗(yàn)動(dòng)為：取L2號(hào)普通注射針頭，首先將針頭尖部去掉，然物實(shí)施不同的給藥方法。尤其對(duì)于藥物的毒理、藥后用細(xì)銼刀把去掉部分銼平、銼光，再用沙紙磨光效的研究和藥物劑量的確定，經(jīng)口灌胃給藥方法是滑，有條件時(shí)可在針尖處點(diǎn)焊成球型加工成有20。一種重要的途徑。在國(guó)內(nèi)文獻(xiàn)上卻很少見(jiàn)到具體的度弧度的灌胃針，連接第一或5ml的注射器。即成為描述。在此，作者將在實(shí)踐中摸索出的一套實(shí)用于大小鼠的灌胃器。在打磨針頭或加工弧度時(shí)用力一小鼠、大鼠、豚鼠、家兔經(jīng)口灌胃給藥的方法介紹如定要均勻，否則就會(huì)將針管弄扁，影響針管暢通。豚下鼠灌胃器，可采用大鼠灌胃器。1.2家兔灌胃器的制作1灌胃器具制作取號(hào)腰椎穿刺針加工方法同小鼠、大鼠灌I2。1.1小鼠、太鼠、豚鼠灌胃器的制作胃器的制作，針體長(zhǎng)約9cm。Andconnect10ndorI1InotesEjector,thatis,rabbitstomachpump.Abouttheauthor:ZhaoMingde(1968,1),male,experimenterPageand2VIPinformationThirdstageZhaoMingde:commonlyusedsmallexperimentalanimalsintragastricadministrationtechniquesintroduced259TheMinistryopensthemouth.Then,therighthandtoholdagoodinfusionofliquidmedicinestomach2gastriclavageRightfromthemouthslowlywitharcinsertion.Iftheinsertionpositioniscorrect,2.1micewereorallyadministeredbygavage,andtheycouldseetherabbitsswallowingautomatically.Iftheinsertionwasnotright,theanimalswouldmoveFirstBaodinganimal,thatis,withtherighthandtoliftthetailofmice,whenplacedinratsshouldimmediatelypulloutthestomach,reinsertthestomachintothestomach,theneedleintothestomachCageorroughsurface,whenthemousestruggledforward,withthelefthandthumbdepthof15~18cm.Afterthemedicineisfinished,thestomachispulledoutslowly.Fingerandforefingerintwoearsafterneckskin,usethismethodtoflipthemouseintotherabbitIg,basicallynotthedruginjectionerrorPalm,straightenthehindlegs,withasmallfingertokeepthetailofmice.TracheainBaoding.Themaximumamountofintragastricadministrationofrabbitis80-15Oral/only.Intheprocessofmice,donotforcetoomuch,donotholdtheneck,soasnottosuffocate3mattersneedingattentionDeath.Thestomachisgentlypressedtothehead,sothatthemouthandesophagusbecomeoneAstraightline,andthenbygavageneedlegentlyalongtheroofwallintotheesophagus,whenadministered3.1miceandratsWhentheneedlereachesabout3cI'/L,itreachesthestomach.IfthepositionoftheneedleisplacedinBaoding,theanimalsmustbefixedsothattheanimalheadsdonotInsertthecorrectmouse,toswallowdrugs,gavageneedleinsertionpositionisnottoswingfreely,butcannotsqueezetheneck.ThesyringeneedlefacesinradiansIndeed,themicewillstrugglestronglyandmustpullouttheplug.Otherwise,theymaybeinsertedintotherightcorneroftheanimalbeforethedrugistaken,andthemovementsshouldbegentle.IlltreatmentThesubstancewasinjectedintothetrachea,causingdeathinmice.Aftertakingtheliquidmedicine,gentlypullouttheanimals,especiallytherats,orbitethemeasily.InsertintothestomachGastriclavageneedle.Themaximumintragastricadministrationtimewas0.4ml/10g.w.Needle,ifyouencounterresistance,youcannotcontinuetoentertheneedle,shouldbestopped2.2ratswereorallyadministeredbygavage,andtheneedleswerewaitingforautomaticswallowing.Otherwise,it'seasytodamageThemethodofcaptureinratsisbasicallythesameasthatinmice.Dependingonthelengthoftheanimal'sesophagus.Practicalexperience,wefound,suchascoveredwithapieceofwetgauzeintherat,3.2guineapigsTheutilitymodelhastheadvantagesofskidresistanceandsafetyforbeginners.Inordertoguineapigtemperamentisgentle,buttimid,easytobesurprisedTopreventratbite,wearcanvasgloves.Theratswerelargerandfrightened.So,whencatchingguineapigs,bequickandsoft.BaodingMobileYoucan'tpressyourtaillikeamouseinBaoding.Similarly,inthepreservationoftheobject,itshouldbecoveredwithagauzehead.Fixinganimals,tomakethemRatscannotbeforcedtoolargeandholdtheirneck,especiallyinthegrip,cannotbearbitraryswing.Whenthestomachisfilled,thestomachcannotbeinsertedTakespecialcareoftheBaodingrats.Thekeytothegastriclavageinratsisthelargeleftanddonotdamagetheesophagus.Theheadoftheratisfixedsothattheheaddoesnotswingatwill.Other3.3rabbitsThemethodofoperationisbasicallythesameasthatofmice.Fillthestomachneedleintothedepthof5cmBaodingrabbits,mustmakeitsheadcannotarbitrarilyswing,todoAbout。Themaximalcapacityofratswas2ml/lOOg.watonetime.Tothispoint,thelefthandtoforcestuckaroundthemouthoftherabbit.InsertintothestomachTwo3guineapigsareorallyadministeredbyintragastricadministration.Thepositionofthedeviceshouldbecorrect.Donotmissthetrachea.Puttheguineapigontheexperimentaltableandcoveritwithadrygauze,FixtheguineapigunderyourlefthandandpressitwithyourthumbandforefingerLetthemouthopen.Then,therighthandisholdingthepumpingirrigationstomachmedicine,alongthedolphinreferenceTheratpalateinsertedintotheesophaguswall.Ifthestomachisinsertedintothepositionissmooth,1.initiative.Modernmedicallaboratoryanimalscience[M].Beijing:People'smilitaryIndeed,theguineapigautomaticallyswallowsthesecondcause.Otherwise,theguineapigwouldmove,andthenpressthepress,September2000.307-314.Pulloutstomach,backintothestomach,gastriclavagedevicetothedepthof2.ZhuQinghua,ZhuQingfan.LaboratoryAnimalScience[M.Guangzhou:GuangdongUniversityEduc