雞傳染性支氣管炎新版

雞傳染性支氣管炎哈爾濱獸醫(yī)研究所禽傳染病研究室劉勝旺雞傳染性支氣管炎新版第1頁1.傳染性支氣管炎病毒2.傳染性支氣管炎3.傳染性支氣管炎疫苗4.LDT3-A疫苗雞傳染性支氣管炎新版第2頁型眾多：傳染性支氣管炎病毒型眾多，已發(fā)覺全世界超出100個(gè)以上型4/91-like*Mass-type*Conn-like*LX4-typeorQX-like*CK/CH/LSC/99I-type*CK/CH/LDL/97I-type*雞傳染性支氣管炎新版第3頁IBV分型方法ClassificationsystemsofIBVaredividedintotwomajorgroups:functionaltests,whichregardthebiologicalfunctionofavirus;andnon-functionaltests,whichlookattheviralgenome.Typingbyfunctionaltestsresultsinimmunotypesorprotectotypes,andantigenictypes(serotypesandepitope-types).Testswhichlookatthegenomeresultingenotypes.DeWit,Avianpathology,,29:71-93雞傳染性支氣管炎新版第4頁RelationshipamongtypesofIBVRelationshipbetweenserotypesandgenotypesbasedonthehypervariableregionoftheS1geneofinfectiousbronchitisvirus:norelationshipexistsbetweenpathotypeandgenotype.--WangCH,HuangYC.ArchVirol.;145(2):291-300.----把“致病型”作為疫苗選擇標(biāo)準(zhǔn)，是長(zhǎng)久以來中國(guó)IBV疫苗選擇一個(gè)誤區(qū)InfectiousbronchitisvirusS1genesequencecomparisonisabetterpredictorofchallengeofimmunityinchickensthanserotypingbyvirusneutralization.--Ladman,B.S.,A.B.Loupos,andJ.Jr.Gelb.AvianPathol.35:127-133..雞傳染性支氣管炎新版第5頁美國(guó)Massachusetts:十九世紀(jì)四十年代，幾乎全世界養(yǎng)雞國(guó)家都存在Connecticut：1951年，與Mass沒有交叉保護(hù)，有Conn疫苗Arkansas：1973年，1983年培育出疫苗Colifornia99：1999年，Mass-Conn二聯(lián)活疫苗對(duì)其有保護(hù)Delaware072：1992年，與D1466含有親緣關(guān)系，有疫苗Georgia98:1998年，年研發(fā)出疫苗GA08：年常見血清型2.傳染性支氣管炎病毒流行展現(xiàn)地域性---慣用當(dāng)?shù)赜蛄餍胁《具M(jìn)行疫苗研制和使用雞傳染性支氣管炎新版第6頁歐洲Mass：1970年在歐洲普遍存在D207（D274）、D1466、D3896和D3128：1984年在荷蘭有疫苗B1648：1990年比利時(shí)，腎型4/91：1990年，歐洲普遍存在Italy02：年發(fā)覺與意大利，現(xiàn)在歐洲普遍存在LX4型(QX)：歐洲普遍存在常見血清型雞傳染性支氣管炎新版第7頁LX4分布世界1997年中國(guó)年東歐年西歐年荷蘭年斯洛文尼亞年俄羅斯年津巴布韋年韓國(guó)（K-II）年日本（JP-III）n年馬來西亞（Malaysia）年瑞典年意大利年英國(guó)中國(guó)起源：上世紀(jì)九十年代首先發(fā)覺于我國(guó)，當(dāng)前是流行于歐洲主要血清型傳染性支氣管炎病毒之一；分布：自1999年發(fā)覺至今，分布于全國(guó)幾乎全部養(yǎng)雞場(chǎng)，是我國(guó)流行第一血清型傳染性支氣管炎病毒；數(shù)量：超出80%病例都是該血清型病毒引發(fā)雞傳染性支氣管炎新版第8頁CK/CH/LSC/99I型起源：上世紀(jì)九十年代首先發(fā)覺于我國(guó)，在其它國(guó)家未見報(bào)道；分布：自1995年發(fā)覺至今，該型病毒主要存在和流行于我國(guó)四川省和重慶市，當(dāng)前是流行于我國(guó)第二主要血清型傳染性支氣管炎病毒；數(shù)量：超出10%病例都是該血清型病毒引發(fā)Mass型起源：上世紀(jì)八十年代首先發(fā)覺于我國(guó)，該血清型病毒在幾乎全部養(yǎng)雞國(guó)家都存在和流行，是世界范圍內(nèi)主要血清型傳染性支氣管炎病毒之一；分布：最初在我國(guó)發(fā)覺至今，分布于全國(guó)幾乎全部養(yǎng)雞場(chǎng)，即使該血清型疫苗在我國(guó)應(yīng)用了超出20年，但該病依然零星流行；數(shù)量：近7%病例都是該血清型病毒引發(fā)雞傳染性支氣管炎新版第9頁CH/CH/LDL/97I型起源：上世紀(jì)九十年代末期首先發(fā)覺于我國(guó)，被認(rèn)為是引發(fā)“腺胃炎”傳染性支氣管炎病毒；分布：自1997-年流行，后再未發(fā)覺該血清型病毒，但年我們?cè)谏綎|省某雞場(chǎng)又發(fā)覺該病毒感染，主要流行于我國(guó)遼東半島；數(shù)量：超出2%病例都是該血清型病毒引發(fā)其它型及變異株在我國(guó)分布范圍很廣雞傳染性支氣管炎新版第10頁3.不一樣型之間之間交叉保護(hù)性較低---疫苗選擇以與當(dāng)?shù)赜蛄餍胁《拘拖嗤蛳嗤瑸闃?biāo)準(zhǔn)雞傳染性支氣管炎新版第11頁ck/CH/LHB110825(KJ425488)

ck/CH/LNM/091017(JF330899)ck/CH/LHB/131132(KJ425500)ZJ971(EU714028)ck/CH/LHB/131143(KJ425502)H120(FJ807652)ck/CH/LSD/110529(KJ425511)ck/CH/LSD/111241(KJ435284)ck/CH/LHLJ/131216(KJ425507)ck/CH/LHB/111232(KJ425490)ck/CH/LHB/120749(KJ425493)ck/CH/LHB/130642(KJ425498)ck/CH/LSD/111228(KJ435285)ck/CH/LDL/120557(KJ425486)ck/CH/LHLJ/111050(KJ425506)ck/CH/LHB/131142(KJ425501)H120(GU393335)ck/CH/LHB/111172(KJ425489)ck/CH/LSD/111219(KJ435283)ck/CH/LHLJ/090908(KJ425503)ck/CH/LSD/110726(KJ425512)ck/CH/LHB/121040(KJ425495)ck/CH/LHB/121024(KJ425494)ck/CH/LHLJ/110310(KJ425505)ck/CH/LHB/131118(KJ425499)ck/CH/LHB/110526(KJ425487)ck/CH/LHB/111268(KJ425491)ck/CH/LJL/121059(KJ425509)ck/CH/LSD/110505(KJ425510)H120(FJ888351)ck/CH/LHB/120403(KJ425492)ck/CH/LHB/130598(KJ425497)ck/CH/LHN/090909(KJ425508)

ck/CH/LHLJ/07VII(JF274479)M41(DQ834384)H52(EU817497)Mass41vaccine(GQ504725)Mass411972(FJ904721)Mass411979(FJ904722)ck/CH/LSD/1112150(KJ435286)

ck/CH/LHLJ/100902(JF828980)KQ(AY641576)ck/CH/LHLJ/091205(KJ425504)Massachusetts(GQ504724)Mass41(AY851295)Mass411985(FJ904723)Mass411965(FJ904720)ck/CH/LDL/110931(KJ425485)Mass41(FJ904713)Ck/CH/LHB/130573(KJ425496)Beaudette(KC008600)9999686765999999991837659071886768197136697255579567503947570.02H120-likevirusesMass41-likevirusesck/CH/LGD/120905(FJ904720)4.IBV之間極易發(fā)生重組雞傳染性支氣管炎新版第12頁06.5246ck/CH/LSD/110529ck/CH/LSD/11124199.5H120(FJ807652)ck/CH/LHB/131143ck/CH/LHB/131132ZJ971(EU714028)97.6ck/CH/LNM/091017(JF330899)ck/CH/LHLJ/090908ck/CH/LHLJ/131216ck/CH/LHB/110526ck/CH/LHB/11082599.1ck/CH/LHB/111268ck/CH/LHB/120749ck/CH/LDL/101212(JF828981)99.5ck/CH/LHB/121024ck/CH/LHB/121040100.0ck/CH/LSD/11050599.4ck/CH/LHLJ/110310ck/CH/LHB/120403ck/CH/LHB/13111895.7ck/CH/LSD/110726H120(FJ888351)H120(GU393335)98.6ck/CH/LDL/120557ck/CH/LSD/12122899.7ck/CH/LHB/111232ck/CH/LHLJ/11105099.8ck/CH/LHB/130642ck/CH/LJL/121059ck/CH/LHB/131142ck/CH/LHN/09090998.8ck/CH/LHB/111172100.0ck/CH/LDL/110931ck/CH/LHB/13057399.4ck/CH/LHB/13059898.399.9H52(EU817497)99.4Mass41vaccine(GQ504725)99.4Mass41(FJ904713)100.0Mass411965(FJ904720)Mass411985(FJ904723)99.5Massachusetts(GQ504724)99.6ck/CH/LHLJ/091205Mass41(AY851295)94.4ck/CH/LSD/1112150ck/CH/LHLJ/100902(JF828980)100.097.5ck/CH/LHLJ/07VII(JF274479)M41(DQ834384)100.0100.0KQ(AY641576)100.0Mass411972(FJ904721)Mass411979(FJ904722)100.0100.0100.0Beaudette(KC008600)98.2ck/CH/LSD/111219H120-likevirusesMass41-likevirusesck/CH/LGD/120905雞傳染性支氣管炎新版第13頁24159-255265′1-2414225536-276303′ck/CH/LHB/111172

ck/CH/LHB/111172H120(GU393335)M41(DQ834384)

ck/CH/LHB/100801(JF330898)TW2575/98(DQ646405)100881

ck/CH/LHB/100801

ck/CH/LHB/111172TW2575/98H120M4199990.02H120ck/CH/LHB/111172M41

ck/CH/LHB/100801TW2575/981001000.01ck/CH/LHB/111172ck/CH/LHB/111172100H120H12099.7100M41M4191.891.8100ck/CH/LHB/100801ck/CH/LHB/10080187.086.986.7100TW2575/98TW2575/9886.786.786.594.5100ck/CH/LHB/111172ck/CH/LHB/111172100H120H12088.7100M41M4186.292.6100ck/CH/LHB/100801ck/CH/LHB/10080199.988.586.2100TW2575/98TW2575/9891.488.587.091.4100ck/CH/LHB/111172ck/CH/LHB/111172100H120H12099.7100M41M4195.195.3100ck/CH/LHB/100801ck/CH/LHB/10080183.983.983.7100TW2575/98TW2575/9885.585.585.393.510024142(168)

▼H120(GU393335)---------------------------------------------------------------------------T--T--------------G-------T-----------------G-----CA----ck/CH/LHB/111172TTGACTATTGTTATTACAGAGGAGGTAATTTTTGGGAAATAGAGTCAGCTGAAGATTGTTCAGGTGATGATGAATGTACTGAATAAGTCGCTACAGGAGAACGGAAGTTTTCTAACAGCACTTTATGTATTTW2575/98(DQ646405)

---AGT-----------A---------G—A----------------------C--C--G----------------T--T----------------------------------------------------25526(39)▼H120(GU393335)---------------------------------------A---G-T--T----------------------------------------------------------------------------------ck/CH/LHB/111172ACAAATACAGACGATGAAATGGCTGACTAGTTTTGGAAGGGCATTCATCTCATGTTATAAAGCCCTACTATTAACTCAGTTAAGAGTATTAGATAGGTTAATTTTAGATCACGGACCAAAACGCGTCTTAATW2575/98(DQ646405)-------------------------------------------------------------T--------C----A--A--GC-GT----------------------AT-------T--C---AGTC---雞傳染性支氣管炎新版第14頁5′1-2148721527-275823′ck/CH/LDL/110931ck/CH/LDL/110931ck/CH/LDL/110931100H120H12099.8100M41M4191.291.2100Conn461972Conn46197292.392.292.5100Conn461996Conn46199692.392.292.599.8100ck/CH/LDL/110931ck/CH/LDL/110931100H120H12090.8100M41M4191.095.5100Conn461972Conn46197299.990.790.9100Conn461996Conn46199699.990.991.199.9100Conn461972(FJ904717))Conn461996(FJ904716)M41(DQ834384)

ck/CH/LDL/110931H120(GU393335)1001000.01

ck/CH/LDL110931Conn461972Conn461996H120M41100950.0121487(1174)▼H120(GU393335)----------------------------------------------------------------------------------------------------------------------------------------G-------------ck/CH/LHB/110931CAATCTGTCTTTAGTGGTAGAGCAACCTGTTGTTATGCTTACTCATATGGAGGTCCTTTGCTGTGTAAAGGTGTTTATTCAGGTGAGTTAGATCATAATTTTGAATGTGGACTGTTAGTTTATGTTACTAAGAGCGATGGCTCTCGTATA

Conn461972(FJ904717)--------------------------T--------------T-----C----------C-T-------G----------T----------A-AAG-G-----------------------------------------------------H120(GU393335)------------------------------------------------------------------------------------------------------------------------------------------------------ck/CH/LHB/110931CAAACAGCCACTGAACCGCCAGTTATAACTCAACACAATTATAATAATATTACTTTAAATACTTGTGTTGATTATAATATATATGGCAGAACTGGCCAAGGTTTTATTACTAATGTAACCGACTCAGCTGTTAGTTATAATTATCTAGCA

Conn461972(FJ904717)------------------------------------------------------------------------------------------------------------------------------------------------------

H120(GU393335)---------T----------------------------------------C------A---------------A---------------------------T------------------------------------------------ck/CH/LHB/110931GACGCAGGTATGGCTATTTTAGATACATCTGGTTCCATAGACATCTTTGTTGTACAAGGTGAATATGGTCTTACTTATTATAAGGTTAACCCTTGCGAAGACGTCAACCAGCAGTTTGTAGTTTCTGGTGGTAAATTAGTAGGTATTCTT

Conn461972(FJ904717)------------------------------------------------------------------------------------------------------------------------------------------------------H120(GU393335)-----------------------C--------------T-----------C-------------------------T-----------------A—G-----GA------------------------T---------------------ck/CH/LHB/110931ACTTCACGTAATGAGACTGGTTCTCAGCTTCTTGAGAACCAGTTTTACATTAAAATCACTAATGGAACACGTCGTTCTAGACGTTCTATTACTGCAAATGTTACAAATTGCCCTTATGTTAGTTATGGCAAGTTTTGTATAAAACCTGATConn461972(FJ904717)------------------------------------------------------------------------------------------------------------------------------------------------------雞傳染性支氣管炎新版第15頁5′1-2167121685-275943′ck/CH/LHB/130573ck/CH/LHB/130573ck/CH/LHB/130573100H120H12099.8100M41M4191.291.1100partridge/GD/S14/partridge/GD/S14/84.684.584.3100ck/CH/LHB/130573ck/CH/LHB/130573100H120H12086.7100M41M4186.595.2100partridge/GD/S14/partridge/GD/S14/99.886.886.6100tl/CH/LDT3/03tl/CH/LDT3/0399.886.786.599.7100H120(GU393335)ck/CH/LHB/130573M41(DQ834384)Partridge/GD/S14/(AY646283)1000.02ck/CH/LHB/130573

tl/CH/LDT3/03(AY02975)PartridgeGDS14H120M41921000.0121671(1358)▼tl/CH/LDT3/03(AY02975)----------------------------------------T------------------GT---T-G---------------------T------T-ATC--------------------------------------------------ck/CH/LHB/130573AACACAATTATAATAATATTACTTTAAATACTTGTGTTGAGTATAATATATATGGCAGAACTGGCCAAGGTTTTATTACTAATGTAACCGACTCAGCTGTTAGTTATAATTATTTAGCAGATGCAGGGTTGGCTATTTTAGATACATCAGH120(GU393335)----------------------------------------T------------------------------------------------------------------------C-------C-----T--------------------T-

tl/CH/LDT3/03(AY02975)-------------------------------------------------------------------------------C----------------------------------------------------------------------ck/CH/LHB/130573GTGCCATAGACATCTTTGTTGTACAAGGTGAACATGGTCTTAATTATTACAAGGTTAATCCCTGTGAAGATGTAAACCAGCAGTTTGTAGTTTCTGGTGGTAAATTAGTAGGTATTCTTACCTCACGTAATGCAACAGGTTCTCAGCCTCH120(GU393335)--T----------------C------A-----T----------------T--------C--T--C--------C-----------------------------------------------T----------AG--T-----C----T--tl/CH/LDT3/03(AY02975)-------------T----------------------------------------------------------------------------------------------------------------------------------------ck/CH/LHB/130573TTGAGAATCAATTCTACATTAAACTCACTAAAGAGACACGTCGTTTTAGACGTTCTACTAGTGATAATGTAACAAGTTGCCCTTATGTAACTTATGGCAGGTTTTGTATACAACCTGATGGTTCTATTAAGCAAATAGTACCGCAAGAATH120(GU393335)----------G--T-----C---A-------T-GA----------------------T--C---A-G---TGA--A------------T-G------T-A----------A----------C--A---GCCAC---------AA--C---雞傳染性支氣管炎新版第16頁5′1-2534725359-276093′ck/CH/LHB/130598ck/CH/LHB/130598ck/CH/LHB/130598100H120H12099.8100M41M4192.091.91004/914/9192.091.989.2100ck/CH/LHB/130598ck/CH/LHB/130598100H120H12088.1100M41M4187.194.01004/914/91100.088.287.2100ck/CH/LHB/130598H120(GU393335)M41(DQ834384)4/91(KF377577)1000.01ck/CH/LHB/1305984/91H120M41990.024/91(KF377577)--------T----------------------------TA--------T-------------------------A---A-----------------TG-C-----------C---------T-----------AT----------------ck/CH/LHB/130598TCAGTAGACACTGGCGAGCTAGAAAGTGTAGCAACAGGTGGAAGTAGCCTTTACACATAAATGTGTGTGTGTAGAGAGTATTTAAAATTATTCTTCAATAGTGCCTCTATTTTAAGAGCGCGGAAGAGTATTTGTTTTGAGGATATTAATH120(GU393335)

------------------------------------------------------------------------------------------------------------------------------------------------------25347(119)

▼4/91(KF377577)-----C--------C--CA----------T--G--------------AA------------AC--T-----GCCA--A-C----TG---------G--GTCC-----------C------------T-T---------------------

ck/CH/LHB/130598ATAAATCCTCTTTGTTTTGTACTCTCTTTACAAGAGTTATTATTTAAGCAACAGTTTTTCCTTTCCTTTGTTTGGAAGAAAGTTGTTGTTAATGGTGTAGAATTCCAAGTAGAAAATGGAAAAGTCCACTACGAAGGAAAACCAATTTTCH120(GU393335)

--------------------------------------------------------------------------------------------------------------------------------------------C--C------

4/91(KF377577)

------------------------------------------------------------------------------------------------------------------------------------------------------ck/CH/LHB/130598CAAAAAGGTTGTTGTAGGTTGTGGTCTAGTTATAAGAAAGATTAGAATAATTAAACCACCAACAACACTTACTTTTAAAAAGGGCGTTTTATGTTATAAGCGCTTAACAAACACGGACGATGAAATGGCTTACTAGTTTTGGAAGAGCATH120(GU393335)

--------------------------CCA---------G-----A-TGG-----------T---CT--------G---A----------GGAC---C--------------T--A---------------G------------------G雞傳染性支氣管炎新版第17頁ck/CH/LSD/1112195′1-1812218134-2407624080-276543′ck/CH/LSD/111219ck/CH/LSD/111219100H120H12086.4100M41M4187.090.2100CK/CH/SD/09/005CK/CH/SD/09/00596.986.587.1100DY07DY0797.586.587.096.8100ck/CH/LSD/111219ck/CH/LSD/111219100H120H12099.7100M41M4196.496.3100CK/CH/SD/09/005CK/CH/SD/09/00577.377.376.9100DY07DY0784.284.284.079.6100ck/CH/LSD/111219ck/CH/LSD/111219100H120H12085.7100M41M4185.894.4100CK/CH/SD/09/005CK/CH/SD/09/00588.184.984.7100DY07DY0797.885.885.788.0100

ck/CH/LSD/111219DY07(HM245923)

CK/CH/SD/09/005(KF668605)H120(GU393335)M41(DQ834384)1001000.01

ck/CH/LSD/111219H120M41DY07

CK/CH/SD/09/0051001000.02

ck/CH/LSD/111219DY07

CK/CH/SD/09/005H120M411001000.0218122▼H120(GU393335)-----------C--------------------C-----AC----C-----A—C-------------------------------------------------------------------------------------------------ck/CH/LSD/111219TTTTGGAATTGTAATGTGGATTGTTATCCTGAAAATTCGTTAGTTTGTAGGTATGACACACGAAACTTGAGTGTGTTTAATCTACCAGGTTGTAATGGTGGTAGTCTGTATGTGAACAAACATGCATTTCACACACCTAAATTTGACCGCDY07(HM245923)

--------------C--------------------------------------------------T---------------T----T-----------------C--------T--T-----------C-----------G-----T---24076(45)▼H120(GU393335)-------------------A---------------------------------------------------------------------------A-------------------G--------------AT----C---G--G------ck/CH/LSD/111219GATAGTAATTTACAGTCTAGACTGACCCTTTGGCACAGTCTAGACTAATGTTAAACTTAGAAGCAATTATTGAAACTGGTGATCAAGTGATTCAACAAATCAGTTTCAATTTACAACATATTTCAAGTGTTCTAGAAACACAAATATTTGDY07(HM245923)A---A---------A-----G---CT------------C--------------G-T--T--GAA--CA--------A-----A--GT-AG-A----------------------------------------------------------雞傳染性支氣管炎新版第18頁雞傳染性支氣管炎新版第19頁1.傳染性支氣管炎病毒2.傳染性支氣管炎3.傳染性支氣管炎疫苗4.LDT3-A疫苗雞傳染性支氣管炎新版第20頁對(duì)呼吸道危害----“呼吸型”傳支1.單純IBV感染常表現(xiàn)為3種致病型雞傳染性支氣管炎新版第21頁對(duì)腎臟危害---“腎型”傳支腸型-G株雞傳染性支氣管炎新版第22頁Toroetal.,Avianpathology.,35:455-464.2.IBV感染造成免疫抑制IBVisfrequentlyfoundinassociationwithimmunosuppressionasinducedbylymphocyticdepletionofboththethymusandthebursaofFabricius.雞傳染性支氣管炎新版第23頁3.與其它病原混合感染---現(xiàn)地混合感染觸目驚心雞傳染性支氣管炎新版第24頁混合感染：IBV感染和腺胃炎----“腺胃型傳染性支氣管炎”錯(cuò)誤雞傳染性支氣管炎新版第25頁雞傳染性支氣管炎新版第26頁4.蛋雞感染4.1.性成熟以后，尤其是產(chǎn)蛋期蛋雞感染注意與其它呼吸道病毒感染判別診療產(chǎn)蛋雞感染IBV出現(xiàn)呼吸道癥狀產(chǎn)蛋下降蛋殼品質(zhì)無改變大部分蛋雞今后痊愈產(chǎn)蛋率恢復(fù)雞傳染性支氣管炎新版第27頁4.2.性成熟之前感染---假母雞萎縮水泡雞雞傳染性支氣管炎新版第28頁萎縮---產(chǎn)蛋率和蛋品質(zhì)同時(shí)下降雞傳染性支氣管炎新版第29頁雞傳染性支氣管炎新版第30頁萎縮---不產(chǎn)蛋，但雞只健康狀態(tài)良好，所以雞群群體產(chǎn)蛋率低雞傳染性支氣管炎新版第31頁4.種公雞感染人工感染IBV可使公雞生殖能力降低IBV疫苗免疫和強(qiáng)毒感染可造成公雞附睪結(jié)石和附睪炎可在人工感染IBV公雞睪丸和精液中檢出IBV（1）、對(duì)公雞本身影響Cooketal.,J.Comp.Pathol.1974Villarrealetal.,AvianDiseases,雞傳染性支氣管炎新版第32頁取精液接種SPF母