版權(quán)說明:本文檔由用戶提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請進(jìn)行舉報(bào)或認(rèn)領(lǐng)
文檔簡介
微生物外文文獻(xiàn)
TheresistanceoftheyeastSaccharomycescerevisiaetothebiocidepolyhexamethylenebiguanide:involvementofcellwallintegritypathwayandemergingroleforYAP1。
CarolinaElsztein1,RodrigoMdeLucena1andMarcosAdeMoraisJr1,2*
Background
Previousworkhasshownthatpolyhexamethylenebigua-nide(PHMB)waseffectiveinselectivelykillingtheyeaststhatareregardedascontaminantsoftheethanolfermentationprocess,withspecialattentionbeingpaidtoDekkerabruxellensis[1].PHMBhasbeenusedasanantisepticanddisinfectantinmedicineandindustry,anditscurrentapplicationsincludethefollowing:impregnationoffabricstoinhibitmicrobialgrowth[2,3],watertreatment[4],disinfectionofawiderangeofsolidsurfacessuchascontactlenses[5],。
InterdepartmentalResearchGroupinMetabolicEngineering.Av.MoraesRego,1235,CidadeUniversitária,50670-901,Recife,PE,Brazil。
Fulllistofauthorinformationisavailableattheendofthearticle
Elszteinetal.BMCMolecularBiology2011,12:38
surfacessuchasbacterialcellwallsanditsbiocidalmechanismmayincludedamagetothecellmembrane[16].Atlowerconcentrations,ithasbeensuggestedthatthebacteriostaticeffectsofPHMBmaybepartlyduetoapowerfulcooperativeinteractionwith(polya-nionic)nucleicacids[17].ItwassuggestedthatPHMBshouldinteractwithphospholipidsoftheAcantha-moebacellmembraneandthuscausechangesincellpermeability[18,19],andthiswasfurthersupportedbyworkonE.coli[14].TheexternallayerofSaccharo-mycescerevisiaeandotheryeastspeciesplasmamem-braneisenhancedbyphosphatidylcholine,ergosterolandsphingolipids[20].OwingtothecationicnatureofPHMB,itstoxiceffectmaybemediatedbyitslinkstothenegativephospholipidsontheyeastcellsurface.InthepresenceofPHMB,thehomogeneousdistributionofphospholipidsthatareusuallylinkedtobiologicalmembranes,istransformedintoamosaicofindividualphospholipiddomainsthatproducefluidandliquidcrystallineregionsinthecellmembrane[15].Bymeansofthewhole-genometranscriptionalprofile,Allenetal[14]showthatE.colirespondstobacteriostaticlevelsofPHMBbyalteringtheexpressionofmanyofthegenesinvolvedatalllevelsofthecellultrastructure,i.
e.theoutermembrane,periplasm,innermembraneandcytoplasmicdomains,butnotthelipolysaccharidelayer.Therewasalsoanalterationintheexpressionofgenesassociatedwithstressessuchasacidresistance,alkaliresistance,osmoticshockandcell-envelopeperturbation.
AswellasthefungicideactivityinD.bruxellensis,PichiagaleiformesandCandidatropicalis,theyeastspe-ciesisolatedPHMBasacontaminantthroughethanolfermentation,andthisalsodifferentiallyaffectedSac-charomycescerevisiaeindustrialstrains,forexamplethePE-2strainwasmoreaffectedthanJP1[1].ThisresultmightimposeconstraintsontheuseofPHMBincon-trollingincidentsofyeastcontaminationinindustrialfermentations.Thus,thefurtherstepstowardsdevelop-ingindustrialformulationsofPHMB,mustrelyondeterminingtheidentificationofbiologicalactivityandtargetsofPHMBontheyeastcells,andidentifyingtheyeastmechanismsthatrespondtothedamagecausedbythisbiocide.
Inthisstudy,wehavesoughttounderstandtheaspectsofPHMB-cellinteractiondiscussedabove,byadoptingtwostrategies:1)testingthebiocideeffectsofPHMBontheexpressionofyeastgenesinvolvedinthecellwallintegritymechanism(CWI)and2)bytestingyeaststrainswithmutationsingenesinvolvedinCWIandtheiroxidativestressresponse.TheresultsstronglyindicatedthatPHMBmayactbydestabilizingtheyeastcellwall,thusinducingthecellwallintegritypathwayresponse.Moreover,anaccountisgivenofthepotentialregulatoryroleofYap1pontheexpressionofCWIgenes.
ResultsandDiscussion
PHMBtreatmentinducesgenesoftheyeastCellWallIntegrity(CWI)sensingmechanism
OurpreviousresultsshowedthatthelethaleffectofPHMBonyeastcellswaspartlymitigatedbytrehalose[1],whichisknowntobeaprotectiveagentofthecellenvelope[20].Moreover,previousglobalgeneexpres-sionanalysisrevealedthatexposureofE.colicellstoPHMBinducedtheexpressionofgenesinvolvedincellwallmaintenance[14].ThisledustosearchforCWIgenesintheyeastgenome(Additionalfile1).Moreover,recentstudieshaveshownthatsomegenesinvolvedintrehalosemetabolismareregulatedbystressresponseelements(STRE)intheirpromoterregion;[21],inviewofthiswehaveincludedsomeyeastgenescontainingSTREintheirpromoterinouranalysis.Fromalistofselectedgenes(Additionalfile1),weconductedaquan-titativePCRanalysisaftercellexposuretoPHMBandtheresultsrevealedthattheCHS1,FKS1,GAS1,HSP150,KRE6,MSN2,MSN4,PKH1andYLR194cgeneswereup-regulatedinthePHMB-resistantJPIstrain,whileremainingunchangedinthePHMB-sensi-tivePE-2strain(Figure1).Moreover,CRZ1andRLM1geneswereinducedinJP1andrepressedinPE-2,whileCIN5andMNN9didnotchangeinJP1,butwererepressedinPE-2.TheexpressionoftheSLT2Figure1Relativeexpressionsoftheyeastgenesinvolvedinthecellwallintegritymechanismandinthegeneralstressresponse.RelativeQuantityrepresentstheexpressionofthe。
studiedgenesinthePHMB-treatedcells(0.01%)overnon-treatedcellsofthetwoindustrialstrainsJP1(redcolumn)andPE-2(bluecolumn).
generemainedunchangedinboththeindustrialstrains(Figure1).
Crz1pisacalcineurin-dependentzincfinger-typetranscriptionfactorthatbindstothepromotersoftheFKS1andCHS1genesinresponsetocalcofluorandcal-cium,respectively[23].Bothgeneswerefoundtobeco-expressed,withapeakinG1,whentheisotropiccellwallsynthesisalloweddaughter-cellexpansion[23]andourresults(Figure1)corroboratethat
溫馨提示
- 1. 本站所有資源如無特殊說明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請下載最新的WinRAR軟件解壓。
- 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
- 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁內(nèi)容里面會(huì)有圖紙預(yù)覽,若沒有圖紙預(yù)覽就沒有圖紙。
- 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
- 5. 人人文庫網(wǎng)僅提供信息存儲(chǔ)空間,僅對用戶上傳內(nèi)容的表現(xiàn)方式做保護(hù)處理,對用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對任何下載內(nèi)容負(fù)責(zé)。
- 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容,請與我們聯(lián)系,我們立即糾正。
- 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時(shí)也不承擔(dān)用戶因使用這些下載資源對自己和他人造成任何形式的傷害或損失。
最新文檔
- Windows Server 2022活動(dòng)目錄管理實(shí)踐( 第2版 微課版)-課件項(xiàng)目18 在活動(dòng)目錄中實(shí)現(xiàn)資源發(fā)布
- Python程序設(shè)計(jì) 課件 第七章 異常處理
- 北師大版八年級生物上冊專項(xiàng)素養(yǎng)綜合練(五)跨學(xué)科實(shí)踐活動(dòng)課件
- 全國賽課一等獎(jiǎng)英語七年級上冊(人教2024年新編)《Unit 2 Section B(Project)》課件
- DB34T 4933.2-2024陸基圓池養(yǎng)殖技術(shù)規(guī)程 第2部分:鱖
- 四川省廣安市2024年中考化學(xué)真題(含答案)
- 內(nèi)蒙古赤峰市翁牛特旗重點(diǎn)中學(xué)2024屆中考一模數(shù)學(xué)試題含解析
- 四川省成都市樹德實(shí)驗(yàn)中學(xué) 2024-2025學(xué)年七年級上學(xué)期期中數(shù)學(xué)試卷(無答案)
- 八年級生物期中模擬卷(全解全析)(云南專用)
- 初中語文閱讀課件教學(xué)課件
- 精神障礙患者監(jiān)護(hù)人協(xié)議書
- 閱讀SPIN銷售巨人教學(xué)課件
- 信息技術(shù)學(xué)科核心素養(yǎng)教學(xué)課件
- 醫(yī)聯(lián)體實(shí)施計(jì)劃方案
- 《建筑裝飾工程造價(jià)》考試復(fù)習(xí)題庫(含答案)
- 順豐物流發(fā)貨打印單模板
- 250mw燃煤電廠煙氣除塵脫硫工程設(shè)計(jì)大學(xué)本科畢業(yè)論文
- 2023年《病歷書寫基本規(guī)范》年度版
- 工程未完工情況說明范文(共18篇)
- T2FLAIR增強(qiáng)的應(yīng)用 T2FLAIR增強(qiáng)的應(yīng)用
- 圖解透視圖畫法
評論
0/150
提交評論