瓊脂糖凝膠純化回收試劑盒

瓊脂糖凝膠純化回收試劑盒產(chǎn)品特點(diǎn)：離心吸附柱內(nèi)硅基質(zhì)膜全部采用進(jìn)口世界著名公司特制吸附膜，柱與柱之間吸附量差異極小，可重復(fù)性好?？朔藝?guó)產(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。使用了優(yōu)質(zhì)溶膠液，不含傳統(tǒng)溶膠液的碘化鈉和高氯酸鹽，不抑制回收后酶切、連接克隆等下游反應(yīng)。溶膠液調(diào)制成為了黃顏色，便于觀察溶膠效果和監(jiān)測(cè)pH值變化從而達(dá)到最佳結(jié)合效果，大大提高回收效率。

改進(jìn)的溶膠液配方,大大提高了緩沖能力和穩(wěn)定性，即使樣品變化很大也能將PH緩沖在最佳結(jié)合范圍內(nèi)。

快速、方便，不需要使用有毒的苯酚、氯仿等試劑，也不需要乙醇沉淀。產(chǎn)品介紹：

在高離序鹽存在的情況下，DNA片斷選擇性的吸附于離心柱內(nèi)的硅基質(zhì)膜上，再通過(guò)一系列快速的漂洗－離心的步驟，漂洗液將引物、核苷酸、蛋白、酶等雜質(zhì)去除，最后低鹽、高pH值的洗脫緩沖液將純凈DNA從硅基質(zhì)膜上洗脫。發(fā)表文章：1.Low-temperature-inducedExpressionofa3FattyAcidDesaturaseGene(3FAD)fromMyrmeciaincisainSaccharomycescerevisiae.JournalofAgriculturalBiotechnology,2012,20(7):735-7442.AMolecularPhylogeneticStudyofChineseWildVitis(Vitaceae)BasedonInternalTranscribedSpacerandChloroplastDNASequence.FenziZhiwuYuzhong(Online),2011,9:1570-15783.HeterogeneousExpressionofEpoxideHydrolaseGenesfromPopulusTomentosaandApplicationoftheEnzymeforBiocatalyticResolutionofChiralEpoxides.ChineseJournalofCatalysis,2012,33(2):302-3074.Characterizationofthecompletemitochondrialgenomeofthewildsilkwormmoth,2Actiasselene.Gene,2012,505(2):291-299ExpressionofKeratinocyteGrowthFactor-1(KGF-1)inTransgenicSafflower.ChineseBulletinofBotany,2011,46(3):311–318ThecompletemitogenomeofBombyxmoristrainDazao(Lepidoptera:Bombycidae)andcomparisonwithotherlepidopteraninsects.Genomics,2013,101(1):64-73DNA提取試劑盒發(fā)表文章：1.GeneticvariationbetweentwoTibetanmacaque(Macacathibetana)populationsintheeasternChinabasedonmitochondrialDNAcontrolregionsequences.MitochondrialDNA,2012,PostedonlineonDecember17,20122.Azospirillumhumicireducenssp.nov.,anitrogen-fixingbacteriumisolatedfromamicrobialfuelcell.INTERNATIONALJOURNALOFSYSTEMATICANDEVOLUTIONARYMICROBIOLOGY,PublishedonlineaheadofprintDecember21,2012,3.Researchonresuscitationofviablebutnon-culturablestateE.coliO157:H7.FoodScience,2012,OnlineAheadofPrint:Dec11,20124.CharacterizationofmarinePseudomonasspp.antagonisttowardsthreetuber-rottingfungifromJerusalemartichoke,anewindustrialcrop.IndustrialCropsandProducts,2013,43:556–5615.CompleteGenomeSequenceofKlebsiellapneumoniaePhageJD001.JournalofVirology.2012,86(24):13843

提取噬菌體基因組DNA6.CompleteGenomeSequenceofWide-Host-RangeStaphylococcusaureusPhageJD007.JournalofVirology.2012,86(24):13880-13881

提取噬菌體基因組DNA7.CompleteGenomicSequenceofaMuscovyDuck-OriginReticuloendotheliosisVirusfromChina.JournalofVirology.2012,86(23):13140-13141

提取病毒DNA8.LIJia-Yi,YAOYong-Fang,ZHOULiang,XUHuai-Liang.PolymorphicanalysisofMhc-DPB1geneexon2inTibetanmacaques(Macacathibetana).HEREDITAS(Beijing).2012,34:AvailableonlineOctober9.Qiu-NingLiu,Bao-JianZhu,Li-ShangDai,Chao-LiangLiu.ThecompletemitogenomeofBombyxmoristrainDazao(Lepidoptera:Bombycidae)andcomparisonwithotherlepidopteraninsects.Genomics.2012,Availableonline13October2012.

提取線粒體DNA10.CharacterizationofmarinePseudomonasspp.antagonisttowardsthreetuber-rottingfungifromJerusalemartichoke,anewindustrialcrop.

IndustrialCropsandProducts,(2013)43:556–561

11.DevelopmentofNovelVisual-plusQuantitativeAnalysisSystemsforStudyingDNADouble-strandBreakRepairsinZebrafish,JournalofGeneticsandGenomics,JingangLiu,LuGong,ChangqingChang,CongLiu,JinrongPeng,JunChen,Availableonline23August201212.Thecompletemitochondrialgenomeofthewildsilkwormmoth,Actiasselene,GENE,2012Qiu-NingLiu,Bao-JianZhu,Li-ShangDai,Guo-QingWei,Chao-LiangLiuAccepted3June2012.Availableonline8June2012.提取線粒體DNA13.MolecularcharacteristicsofHSC70geneanditsexpressioninthegoldenapplesnails,Pomaceacanaliculata(Mollusca:Gastropoda),Aquaculture（2012）358-359：41-49GuowanZheng,ShengzhangDong,YunHou,KeYang,XiaopingYu,Accepted28June2012.Availableonline10July2012.14.CharacterisationofantioxidantandantiproliferativeacidicpolysaccharidesfromChinesewolfberryfruits

NianwuHea,XingbinYanga,,,YadongJiaoa,LingminTianb,YanZhaoc,,

aKeyLaboratoryofMinistryofEducationforMedicinalResourceandNaturalPharmaceuticalChemistry,CollegeofFoodEngineeringandNutritionalScience,ShaanxiNormalUniversity,Xi’an710062,China

bLaboratoryofFoodChemistry,WageningenUniversity,Bomenweg2,6703HDWageningen,TheNetherlands

cSchoolofPharmacy,FourthMilitaryMedicalUniversity,Xi’an710032,ChinaFoodChemistry

Availableonline14February2012

Received12November2011.Revised21January2012.Accepted3February2012.Availableonline14February2012.15.Analysis

of

genetic

diversity

in

the

wild

populations

of

Trachidermusfasciatus

by

RAPD

and

the

transformation

of

two

SCAR

markers.

Zoological

Research

2012，Apr.

33(2):

1-816.Vibriozhanjiangensissp.nov.,isolatedfromseawaterofshrimpfarmingpond.AntonievanLeeuwenhoek

2011,Publishedonline29December2011質(zhì)粒小量快速提取試劑盒離心吸附柱內(nèi)硅基質(zhì)膜全部采用進(jìn)口世界著名公司特制吸附膜，柱與柱之間吸附量差異極小，可重復(fù)性好。克服了國(guó)產(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。

快速、方便，不需要使用有毒的苯酚、氯仿等試劑，也不需要乙醇沉淀。獲得的質(zhì)粒產(chǎn)量高、純度好，可以直接用于酶切、轉(zhuǎn)化、PCR、體外轉(zhuǎn)錄、測(cè)序等各種分子生物學(xué)實(shí)驗(yàn)。

2×TaqPCRMasterMix1.國(guó)內(nèi)獨(dú)家采用全自動(dòng)發(fā)酵罐表達(dá)，醫(yī)藥級(jí)純化設(shè)備大規(guī)模生產(chǎn)的高純高活性Taq酶。消除了批次之間的差異。

2.為了保證質(zhì)量，核心原料之一的dNTP全部采用昂貴的高純度原裝進(jìn)口dNTP。

3.多年研發(fā)改進(jìn)凝結(jié)成的超穩(wěn)定配方，添加多種穩(wěn)定劑，增強(qiáng)劑，可以反復(fù)凍融不降低活性。負(fù)20冰箱幾乎可以永久保存。

4.經(jīng)過(guò)實(shí)際檢測(cè)，使用本公司Mix擴(kuò)增效果一般是單純使用Taq酶效率的一倍甚至幾倍以上。擴(kuò)增效率和穩(wěn)定性處于業(yè)內(nèi)領(lǐng)先水平。2xSYBRGreenqPCRMix(熒光染料法)

制品說(shuō)明：本制品本制品是采用SYBRGreenI嵌合熒光法進(jìn)行RealTimePCR的專(zhuān)用試劑。已經(jīng)將熱啟動(dòng)HotMasterTaqDNA聚合酶、dNTP、特殊穩(wěn)定劑、優(yōu)化的反應(yīng)緩沖液、BSA和SYBRGreenI等試劑預(yù)混成一種適合RealTimePCR反應(yīng)檢測(cè)用2×PremixType試劑，具有靈敏度高、特異性強(qiáng)、穩(wěn)定性好等特點(diǎn)。使用時(shí)只需加入模板和引物和水，便可在寬廣的定量區(qū)域內(nèi)得到良好的標(biāo)準(zhǔn)曲線，對(duì)目的基因進(jìn)行準(zhǔn)確定量檢測(cè)，重復(fù)性好，可信度高。本品采用全新的HotmasterTaqDNA聚合酶，該酶不同于一般Hot-start酶之處在于