獸醫(yī)專業(yè)英語（揚(yáng)州大學(xué)）l

LessonFifteenSalmonellaTesting

精選pptNewwordsandphrasesSalmonellaBacteriologicalImmunologicalEnzymicChromogenicImmunochromatographicListeriaFloraAgarPeptoneMagneticStreakLuminescentReagentGiardiaCryptosporidiumProtozoa(zoon)StreptavidinImmunosorbentBrothImmobilizeInoculation精選pptTextThefoodmarketcontinuestogrowwiththeintroductionofnewproductsandnovelmethodsofpresentation.Theincreaseinsamplenumbershasprovidedimpetus(推動力)forthedevelopmentofnewtestswhicharedesignedtostreamlinethetestmethodsandprovidereliableandeconomicalresultstoensureboththebacteriologicalsafetyandlong-termstabilityofthefoodproducts.精選pptImmunological,enzymicandmolecularbiologicaltechniquesarenowappearingastestmethodsinadditiontotheroutineagarplatedetectionmethods.Althoughprovidinggreatsensitivityandspecificity,manymethodsstillrequiresignificantcapitalinvestmentinequipmentandskilledpersonnelinordertoattainbesttestperformance.精選pptForSalmonellatestingtheabilitytoprovidequick,sensitiveandaccuratetestresultsisimportanttoensurethatthisdiverseandcommonlyencounteredgroupofpathogensisnotdistributedintothehumanfoodchain.精選pptForqualitycontrolofrawmaterialsthekeyproblemisoftenthedetectionoflownumbersofviablepathogensinthepresenceofmuchgreaternumbersofbackgroundflora.Thisisasignificantproblemifthefinalproductisnotrequiredtoreceivefurthercooking.Incaseswherefoodproductsarepartiallycooked,thenchilledpriortosale,theproblembecomesoneofdetectionrequiringatestmethodwithhighsensitivitytothetargetorganism.精選pptConventionaltestsrequireaperiodofnonselectiveenrichment

followedbyafurtherperiodofenrichmentinaselectivegrowthmedium,whereconditionsfavourgrowthofthetargetorganism,whichcansubsequentlybeculturedbyplatingontoaselectiveagarplate.精選pptThesearchforlownumbersoftargetorganismshasalsobeensignificantlyimprovedbytheuseofimmuno-linkedmagneticbeads,whichcanbeusedto“select”specifictargetorganismsfromamixedculture.Themagneticbeadsarelinkedwithanantibodywhichisspecificforthetargetorganism.Thesebeadsdonotpossessanynativemagnetismbutexhibitmagneticpropertiesonlywhenintroducedintoamagneticfield.精選pptNowtheimmuno-linkedmagneticbeadscanbeusedtocapturetargetorganisms,andthebeadscanthenbeseparatedfromthemixturebyapplicationofastrongmagneticfieldandtheunwantedmixturediscarded.Thebeadpreparationcanthenbewashedandusedforfurtheranalysis,either

bystreakingontoselectiveagarplates

or

bycombinationwithluminescentreagents

inordertorapidlydetectlowlevelsofthetargetorganism.精選pptMagneticbeadsareavailablefordetectionofSalmonella,andE.coliO157:H7.SimilarpreparationsarealsoavailablefordetectionofGiardiaandCryptosporidium,protozoawhichposesignificantproblemsforthewaterindustry.UseofStreptavidinlinkedbeadsprovidesanopportunitytocoatmagneticbeadswithspecificantibodiesofinterest,thusopeningthewayfornewmethoddevelopmentanddetectionoflesscommonmicroorganisms.精選pptAlternativeimmunosorbenttechnologyformsthebasisofaquickandsimpletestdesignedtoscreenenrichmentbrothsamplesforthepresenceofSalmonellaorganisms(antigen).SpecificantibodiesareimmobilizedonapermeablesupportandusedtocaptureSalmonellaantigenswhichmaybepresentinthetestsample.Allthenecessarytestreagentsarepresentinthetestdeviceandthetestcarriedoutsimplybyintroductionofafewdropsofthesampleenrichmentbrothintothetestwell.精選pptThesamplediffusesthroughthereactiontracktogiveatestandcontrolresult----visibleasacolouredlineintheviewingwindow.Testscanbecarriedoutonenrichmentbrothsamplesafteronly24hourswithavisibleresult10~15minutesafterinoculationofthedevice.

精選ppt雞沙門氏菌的分離鑒定及藥物敏感試驗SeparationIdentificationandDrugSusceptibilityTestofAvianSalmonella摘要：從新疆南疆地區(qū)某雞場采集到的14份具有沙門氏菌病病變特征的病料中分離出沙門氏菌10株.Abstract:14sampleswithaviansalmonellosiswerecollectedfromsouthernareaofXinjiang，and10strainsofchickenSalmonellawereseparated．標(biāo)題精選ppt通過形態(tài)觀察、生化試驗、血清學(xué)鑒定及動物致病性試驗，結(jié)果表明，10株沙門氏菌的血清型為O2、O9，這兩種血清型沙門氏菌對5日齡雛雞具有很強(qiáng)的致病作用.Theresultsofmorphologicalobservation，biochemicaltests，serotypeidentificationandpathogenicitytestsshowedthatO2andO9werethedominantserogroupsandbothserogroupswerehighpathogenicforchickens5daysold．精選ppt藥敏試驗結(jié)果表明，沙門氏菌對硫酸慶大霉素最敏感，其次是頭孢唑啉鈉，敏感率分別為100％和90％，其對乙酰螺旋霉素和羅紅霉素產(chǎn)生耐藥性.Thedrugsusceptibilitytestindicatedthatthesensitivitiesofthe10strainstogentamycinsulfateandcefazolinsodiumwere100％and90％，respectively．Andtheywerehighresistancetoacetylspiramycinandroxithromycin．關(guān)鍵詞：雞；沙門氏菌；分離；鑒定；藥物敏感試驗Keywords:chicken；Salmonella；separation；identification；drugsusceptibilitytest精選pptRapidHygieneTestingTechnologyRapidhygienetestingtechnologies,suchasthoseofthemarketleaderBiotrace,aremostlybasedonthemeasurementofadenosinetriphosphate(ATP).ATPischemicalfoundinalivingcells.So,ifATPispresentonasurfacethatappearstobecleantothehumaneye,somefoodresiduesorothercellularmaterialmustbepresentcreatingapotentialbreedinggroundforbacteria.精選pptHarnessingthisprinciple,theATPb