肯博士的法氏囊

LohmannAnimalHealthInternationalGainesville,Georgia,USA

Education73-84’BSCal.PolyPomonaMSPoultrySc,AuburnDVM,U.C.DavisBoardcertified92’KenTakeshitaFosterFarms,CAMgr.HealthServices85-90’ArborAcres,CTDir.PoultryHealth,91-95’HealthofUSAPed.,GGP,GPTechnicalSupportUSAVinelandLabsDirectorTech.Service;95-01’Worldwide

KenTakeshitaHomeofficeVacaville,CAbetweenSac/SFMgr.TechnicalServicesWorldwidesupportConcentratedeffortinAmerica’sLohmannAnimalHealthInternationalLAHIformedinOctober2000MergerofVineland-MBL-LohmannHeadquarters=GainesvilleGALohmannAnimalHealthInternationalEffectiveMay1,2001TechnicalSupportTeamDr.KenTakeshita(USA)Dr.EricLovell(USA)[Consultant]Dr.DavidShapiro(USA)[Consultant]Dr.GregCutler(USA)[Consultant]Dr.CharlesHofacre(USA)[Consultant]Open(USA)Open(LatinAmerica)Dr.HeikeScharr(Germany)Dr.vanDijk(Germany)Dr.CherYew(Singapore)Dr.BeatrizCardoso(Brazil)Dr.KarenBurns(USA),1/1/02Internationaltrips~same;4/yrwhereverneededworldwideLessintensedomestictravelMorefocusoncustomerfollowuppresentations/techbulletinstrialsandotherprojects

InactivatedIBDVaccinesBursalTissueOrigin(BTO)ChickEmbryoOriginCells(CEO)TissueCultureOrigin(TCO)IBDvirusbursaTC(TissueCulture)CEO(ChickEmbryoOrigin)BTO/BD(BursalTissueOrigin)/(BursalDerived)-ChickenEmbryoFibroblast(CEF)-PerpetualcelllinesSignificantvirusadaptationrequired-Infected,wholechickembryosharvestedSomevirusadaptationrequired-SPFchickensinfectedbursasharvestedVirusadaptationnotrequiredGrowingtheIBDvirusBursalTissueOrigin(BTO)orBursalDerived(BD)BTOvaccinesstartedinUK

Early1970’sOriginatedbygroupofUKveterinariansWerenothappywithliveIBDvaccinesaloneProbablychoseBTObecause:EasytogrowGrewtohightiterUKgovernmentrequiredstrictpotencytestsChallengeofchicksfromvaccinatedparentsTheirworkshowedverystrongprotectioninchicksfromvaccinatedparents(to4weeks)VaccineworkwasledbyDr.BoxandproducedbyGlaxoBTOshowedasbettervaccineLongerdurationofimmunitywhencomparedtoTCOandCEOvaccinesInUSA,betterabletoprotectagainstvariantIBDstrainsFlock’sVaccinatedwithBursalDerivedIBD~BetterTitersFlock’sVaccinatedwithBursalDerivedIBD~BetterProtectionBTOshowedasbettervaccineProduction:BecauseNOvirusadaptationisrequired,finaloutput“matches”seeds/”matches”challengeViralgrowthVeryhightitersbecausevirusis“home”PotentiallyotherimmunefactorsproducedSocalled“bursin”IncompleteviralparticlesViruscontentwhichis“hidden”fromnormalassaybursaTC(TissueCulture)CEO(ChickEmbryoOrigin)BTO/BD(BursalTissueOrigin)/(BursalDerived)AdaptationofIBDvirusFieldVirusSignificantvirusadaptationrequiredSomevirusadaptationrequiredNovirusadaptationrequiredBTObecamewidelyusedFirstusedinbreedersasonlyIBDvaccineand/oraspartofprogramwithliveprogenyvaccination1stBTOvaccineswere>2xcostofTCorCEOvaccinesAstimemovedfrom1970’sto1980’sUK&EuropebegantodeclineusageRegulatoryissuesFearofCAVinthevaccinesAnimalwelfare(usingchickenstomakevaccine)InterferencewithlivevaccinesUSAmovedsolidlytoBTOIBDvaccinesUsedaloneand/orwithliveprogenyvaccinationExcellentcoverageofvariantstrainsVaccinecompaniesclaiming“100%BTO”MBL?Vineland

?BursalTissueOrigin(BTO)VinelandKilled4way“new”

1823~novariantIBD~reducecostequalorbettertiters/protection~sameNDV,IBV&Reoprotection“New”LAHI-VinelandKilled4way“1823”BursalDerivedHowdoes“new”1823comparewithotherkilled4wayvaccinesforIBD?*ProVacACLisproducedinacontinuous,aviancellline–tumortransformedquailcellline.**OnlyMBLBTO2andMerialcontain100%BTOIBDantigen.IBDtitercomparisonof

“new”1823*vsother4ways*Prelicensingserial**Birdsprimedwithlivevaccination.Titersmeasured4weekspostvaccinationBursalDerived~bettertitersBursalDerived~BetterProtection…evenwithsmalldoseQuickQuestions…....orontoVi-BursaCEVi-BursaCEVi-BursaCEa“classic”IBDvirusintermediate<->intermediate+invasiveness“non”cloned~broadspectrumprobagatedinChickEmbryo~broadspectrumgoodsafetyingoodprotectioninbroilersandlayersgoodprimerforkilledvaccinationVi-BursaCEisa“Classic”IBDvirusViBursaGclassicIBDintermediate=D78tissuecultureViBursaCEclassicIBD

intermed.=BursineIIchickembryoViBursaLclassic/variantIBDisamildwon’theatupIBDvirusTC(TissueCulture)CEO(ChickEmbryoOrigin)BursineIIVi-BursaCED78Vi-BursaGVi-BursaCEisproducedinCEOVi-BursaCE….multipliedinlymphoidtissuex1Vi-BursaCEx2a2a178Clone“Non”-clonedclonedD78“Non”-clonedVi-BursaLVi-BursaCEis“non”cloned“non”clonedVi-BursaCE….doesn’tloseit’santigenicdiversity78787878a2a1a1a2x1x1x2x278a1x2Vi-BursaCE….issafeVi-BursaCE…goodprotectioninSPFbirdsVi-BursaCE…. GoodprotectioninbroilersVi-BursaCE…. GoodprimingofkilledVi-BursaCE….goodfieldresultsRecentDevelopments

Vi-BursaCEOriginalVi-BursaCEOriginalVi-BursaCE~lessinvasivethanintendedRecentworkonVi-BursaCERe-workedVi-BursaCE~moreinvasivethanoldNewVi-BursaCENewVi-BursaCE~intermediate<->intermediate+Summary;

Vi-BursaCEa“classic”IBDvirus“non”cloned~broadspectrumprobagatedinChickEmbryo~broadspectrumintermediate<->intermediate+invasivenessgoodsafetyingoodprotectioninbroilersandlayersgoodprimerforkilledvaccinationSummaryRecentdevelopments

Vi-BursaCE“originally”wasVineland’smostinvasiveIBDbutbecame“mild”“current”serialsaremoreinvasivethanoldserialsprobablyslightlylowerin“invasive”nessthanBursine+….analternativetoBursine/Bursine+?ViBursaGclassicIBDintermediate=D78tissuecultureViBursaCEclassicIBD

strongerintermed.

likeBursineIIchickembryoViBursaLMildcausesminimalimunosuppressionToBreakThruLAHI-VinelandLiveIBDVaccinesSummaryRecentdevelopments

Vi-BursaCE“originally”wasVineland’smostinvasiveIBDbutbecame“mild”“current”serialsaremoreinvasivethanoldserialsprobablyslightlylowerin“invasive”nessthanBursine+….analternativetoBursine/Bursine+?Vaccination/challengetrialinprogresstodetermineprotectionagainstvariantvsBursineIIconfirminvasivenessofnewserials“WordofCaution”Thinkcarefullybeforesharing“Recentdevelopments”Vi-BursaCEwithendusercanbeanadvantagewithnewcustomeracustomerwhodidnotseeanadvantageusingthe“old”serialsofVi-BursaCEcanbeadisadvantagewithacurrentuserofVi-BursaCE

…..decideaheadoftimeQuickQuestions…....orontoPD50LAHI-Vineland’sNDVPD50PD50PD50orViNuChickVac-KspecificallydevelopedforbabychickvaccinationcalledPD50becauseeachdosecontains50protectivedosebychallengetestisa“High”-potencykilledNDV

large#ofprotectioninsmallvolume/dosepotencyby

WeybridgeProtectiveDoseTestPD50“High”potency=0.1mldose50PD50“High”potency10PD500.5mldose“Average”potencyvs.

birdsvaccinatedwithdecreasingpartialdoseP(rotective)D(ose)50(%)isdosewhichprotects50%ofbirdschallengedwith“Hot”NDVvirusPD50WeybridgeProtectiveDoseTest=0.1and0.05ml100%protection0.025ml92%protection0.0125ml70%protection0.00612ml30%protecciónThisisthePD50pointExtrapolating,onePD50unitofthisvaccineis.0.009mlCalculationofPD50perdose;-Ifdoseis0.1ml~potencyofvaccineinPD50units=0.1ml/0.009=11-theabovevaccineundertesthasapotencyof11PD50perdose.ExampleofdeterminingPD50ofavaccinePD500.1mldose>50PD50PD5022-25PD500.5mldose“Average”potencyvs.

“Average”killedNDVusedinlayersis

=22-25xPD50in0.3-0.5ml

“High”potencyLAHI-Vineland’sPD50forchicksis>50xPD50in0.1mlPD50Purpose;preventhighlyvelogenicNDVmortalityMethod;Simultaneouslive&killedNDV@1day

liveNDVtostimulatesshortlived(3-5weeks)“l(fā)ocal”(IgA)respiratorymucosalliningimmunityhighpotencykilledtostimulateslonglasting“systemic”(IgG)immunity(@least7-8weeks)

note;it’sessentialthatkilledNDV=“High”PotencyPD50Goalof“Simultaneous”live&killedNDVisfor;MiddleEast;reducemortality40%to4%Mexico;simultaneouskilled/liveeffectiveB1live+LAHI-Vineland’sPD50@1dayLaSota@7-10days+/-LaSota@18dayinhighchallengePD50FieldExperiencePD50FieldExperience;SerologyNosignificantantibodytiterafterdayoldkilledOnlyhightitersafterchallengehightiter+mortality/clinicalsigns~poorprotectionhightiter+nomortality/clinicalsigns~goodprotectionlowtiters~nochallengePD50FieldExperience;SerologyPD50FieldExperience;SerologyThailand

@least0.1ml@1dayofage…challengedependent500mlbottle=5,000dosagesinjectIMinlegorSQinneckPD50Recommendations&Usagewait6hrsbetweenMarek’s&LAHI-Vineland’sPD50preferably

vaccinateindifferentsitefromMarek’sCautionLAHI-Vineland’sNDVQuestions?PD50Questions/AnswersQ.Whatisdifferencebetween“highpotency”PD50vsaveragekilledNDVvsStandardKilledNewcastlevaccines.A.TheantigenamountcontainedinPD50isconsiderablyhigherthanthatinakilledNDVusedinolderlayers.TheprotectiongiventothechickensisconsiderablysuperiorusingViNuChickVacKPD50.PD50Questions/AnswersQ.WhyaretheredifferentkilledNDVrecommendedforbroilerchickensandanotheronerecommendedforlayers?

A.1)Smallerdose,0.1vs0.5ml,ispreferredforonedayoldbroilersbecauseofsizeofbirds2)Higherpotencyispreferredfordayoldbroilersvslayersbecauseofhigherearlierchallenge….particularlywherevelogenicNDVispresent.PD50Questions/Ans