醫(yī)學(xué)教材 丙型肝炎病毒(基礎(chǔ))

丙型肝炎病毒邢文革

2014-12-17石家莊丙型肝炎病毒的發(fā)現(xiàn)1970’s:HepatitisAandBdiagnosticsMosttransfusionassociatedhepatitisnotcausedbyHAVandHBVorotherknownviralagents(NEJM292:767;Gastro.69:1278;JID139:511;VoxSang.44:231)non-A,non-Bhepatitis(NANBH)Mightbeasmall,envelopedvirustransmissibletochimpanzees(Gastro88:773;JID156:636)StandardimmunologicalmethodsfailedtoidentifyviralantibodiesorantigensChirongroupThoughtthattherewasnotenoughviralantigeninNANBHSotheydecidedtotryandconcentratethevirus丙型肝炎病毒的發(fā)現(xiàn)1.傳染性肝炎(HA)血清型肝炎(HB)2.動(dòng)物模型（黑猩猩）3.基因文庫(kù)4.放射免疫標(biāo)記5.全基因測(cè)序HightiterNANBHchimpanzeeplasmaUltracentrifugationat104,000gfor5hoursExtractnucleiacid,randomprimedcDNAintogt11phageImmunoscreenedwithNANBHpatientserumClone5-1-1LargeroverlappingClone81丙型肝炎病毒的發(fā)現(xiàn)PhagewithportionsofHCVgenomeInfectE.coliPhagekillsE.coliandleavesplaquesScreenwithHCVPatientserumLibraryScreening丙型肝炎病毒的發(fā)現(xiàn)WesternblotClone5-1-1expressedasSODfusionproteincalledPS5PS5SODNANBHpatientserumExperimentallyinfectedchimpserumProteinfromclone5-1-1closelyassociatedwithNANBHinfectionsHCVwasthefirstvirustobeisolatedandcharacterizedsolelybymolecularmethods,iewithoutculturemethods黑猩猩易感HCV病原學(xué)黃病毒科（Flaviviridae）丙型肝炎病毒屬包膜糖蛋白包膜+ssRNA核衣殼HepatitisCGenome

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