白骨壤NAC家族成員鑒定和功能初探

[32]。RT-qRCR鑒定AmNAC基因在鹽脅迫處理?xiàng)l件下的表達(dá)情況發(fā)現(xiàn)，鹽脅迫下，白骨壤葉中有5個(gè)基因均在處理24h時(shí)呈現(xiàn)上調(diào)表達(dá)，并且基因AmNAC4被顯著誘導(dǎo)，表達(dá)量約為對(duì)照組的3倍。這表明它們可能參與白骨壤應(yīng)答鹽脅迫過程，其中AmNAC4可能起關(guān)鍵作用。RT-qRCR結(jié)果與轉(zhuǎn)錄組數(shù)據(jù)不完全一致，可能是由于樣品生長(zhǎng)環(huán)境不同所致。已有多項(xiàng)研究證實(shí)，NAC轉(zhuǎn)錄因子通過調(diào)控鹽脅迫相關(guān)基因的表達(dá)水平，引發(fā)一系列復(fù)雜的脅迫反應(yīng)，從而提高植物應(yīng)對(duì)高鹽環(huán)境的能力。在植物鹽脅迫調(diào)控中，這些NAC轉(zhuǎn)錄因子主要通過依賴ABA途徑、不依賴ABA途徑或兩者共同參與的方式發(fā)揮作用REF_Ref163408844\r\h[33]。例如依賴ABA途徑的水稻OsNAC3基因，通過調(diào)控ABA響應(yīng)和耐鹽相關(guān)基因的表達(dá)，提高植株對(duì)ABA的敏感性，從而增強(qiáng)植株的耐鹽性REF_Ref163408898\r\h[34]。AmNAC基因共表達(dá)網(wǎng)絡(luò)結(jié)果顯示，5個(gè)鹽脅迫響應(yīng)AmNACs分別與多個(gè)白骨壤基因呈現(xiàn)共表達(dá)趨勢(shì)。以上結(jié)果表明，作為轉(zhuǎn)錄因子，AmNACs可能通過調(diào)控下游基因的表達(dá)進(jìn)而調(diào)控白骨壤鹽脅迫響應(yīng)過程。但AmNAC轉(zhuǎn)錄因子參與鹽脅迫響應(yīng)的途徑及下游靶標(biāo)基因的篩選仍有待進(jìn)一步研究。本研究的成果為篩選受鹽脅迫誘導(dǎo)的靶標(biāo)基因、開展后續(xù)的遺傳轉(zhuǎn)化研究以及深入探索鹽脅迫響應(yīng)功能和分子調(diào)控機(jī)制奠定了理論基礎(chǔ)。

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