GB/T 45033-2024飼用微生物制劑中產(chǎn)朊假絲酵母的測定

ICS

65.120

CCS

B46

中華人民共和國國家標(biāo)準(zhǔn)

GB/T45033—2024

飼用微生物制劑中產(chǎn)朊假絲酵母的測定

DeterminationofCandidautilisinmicrobialfeedadditives

2024-11-28發(fā)布2025-06-01實(shí)施

國家市場監(jiān)督管理總局發(fā)布

國家標(biāo)準(zhǔn)化管理委員會(huì)

GB/T45033—2024

目次

前言

·····································································································

Ⅲ

1

范圍

··································································································

1

2

規(guī)范性引用文件

······················································································

1

3

術(shù)語和定義

···························································································

1

4

試劑或材料

···························································································

1

5

儀器和設(shè)備

···························································································

1

6

采樣

··································································································

1

7

試驗(yàn)步驟

······························································································

2

8

試驗(yàn)數(shù)據(jù)處理

·························································································

3

附錄A（資料性）

培養(yǎng)基

·············································································

5

附錄B（規(guī)范性）

生理生化試驗(yàn)

······································································

6

附錄C（規(guī)范性）

分子生物學(xué)鑒定

···································································

9

Ⅰ

GB/T45033—2024

前言

本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)

定起草。

請(qǐng)注意本文件的某些內(nèi)容可能涉及專利。本文件的發(fā)布機(jī)構(gòu)不承擔(dān)識(shí)別專利的責(zé)任。

本文件由全國飼料工業(yè)標(biāo)準(zhǔn)化技術(shù)委員會(huì)（SAC/TC76）提出并歸口。

本文件起草單位：山東寶來利來生物工程股份有限公司、安琪酵母股份有限公司、北京大北農(nóng)科技

集團(tuán)股份有限公司、浙江科峰生物技術(shù)有限公司、唐山拓普生物科技有限公司、益海嘉里（防城港）生

物科技有限公司。

本文件主要起草人：谷巍、單寶龍、徐海燕、謝全喜、辛國芹、汪祥燕、亓秀曄、覃先武、劉雪連、

章亭洲、周雪玲、馬立周。

Ⅲ

GB/T45033—2024

飼用微生物制劑中產(chǎn)朊假絲酵母的測定

1范圍

本文件描述了飼用微生物制劑中產(chǎn)朊假絲酵母（Candidautilis）菌數(shù)的測定方法。

本文件適用于含有產(chǎn)朊假絲酵母飼用微生物制劑的測定。

2規(guī)范性引用文件

下列文件中的內(nèi)容通過文中的規(guī)范性引用而構(gòu)成本文件必不可少的條款。其中，注日期的引用文

件，僅該日期對(duì)應(yīng)的版本適用于本文件；不注日期的引用文件，其最新版本（包括所有的修改單）適用

于本文件。

GB/T6682分析實(shí)驗(yàn)室用水規(guī)格和試驗(yàn)方法

GB/T42959飼料微生物檢驗(yàn)采樣

3術(shù)語和定義

本文件沒有需要界定的術(shù)語和定義。

4試劑或材料

除非另有規(guī)定，僅使用分析純?cè)噭?/p>

4.1水：GB/T6682，三級(jí)。

4.20.85%滅菌生理鹽水：準(zhǔn)確稱取8.5g氯化鈉加入1000mL水中，攪拌至完全溶解，分裝后，121℃

滅菌30min，備用。

4.3孟加拉紅瓊脂培養(yǎng)基：見附錄A中A.1。

4.4酵母膏胨葡萄糖（YPD）瓊脂培養(yǎng)基：見A.2。

5儀器和設(shè)備

除微生物實(shí)驗(yàn)室常規(guī)滅菌及培養(yǎng)設(shè)備外，其他儀器設(shè)備如下。

5.1培養(yǎng)箱：可控精度±1℃。

5.2天平：感量為0.1g。

5.3光學(xué)顯微鏡：400倍～1000倍。

5.4拍擊式均質(zhì)器及無菌均質(zhì)袋。

5.5旋渦混合器。

5.6無菌培養(yǎng)皿：直徑90mm。

5.