天津醫(yī)科大學(xué)專業(yè)外語(yǔ).ppt

MedicalEnglish Lecture2 EnglishCommunicationinaLaboratory ZhangNingResearchCenterofBasicMedicalSciencesTianjinMedicalUniversitynzhangchina 俯實(shí)玉沼麓撩脊貝錢(qián)澳捻虱撈省雞疙郭蹤蝸糾夫蔣凈曾檸堡懂鈉烙锨輻鈣天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ImproveYourSpokenEnglish EverydayEnglishonlyusesabout2000words youdon tneedtorememberadictionarytospeakEnglishWatchnewsinEnglisheverydayWatchaTVseries suchasFriendsTrytotalktoyourmentorinEnglishTrytodiscussscienceinEnglishwithyourcolleagues 棕綽磺漏廂忿醚萎棋昧安瑟雷付塌倒?jié)B舵對(duì)施涅何燭怎蕪彝餾倦注浙返款天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ImproveYourEnglishWriting ThedifferencebetweenwritinginEnglishandinChineseWritinginEnglishisEasy makeitsimpleandclearMakeyourideaclear topicsentences onepointinoneparagraph Expandandsupportyourargument afewfrequentlyusedwords suggest indicate show demonstrate required sufficient essential contributeto SummaryandSpeculations takentogether insum Revise Revise andRevise 器霸劍注睬穴瞻對(duì)式隴催驗(yàn)頗萎鰓蛹粕眨夷歧孫潰奔翼移瀕溫汪撤做猴垃天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 RecommendedReadingsforEnglishinGeneral BarackObamaDreamsofMyFatherWinstonChurchillMyEarlyLifeBertrandRussellAutobiographyofBertrandRussell 撓判宮嘶豌競(jìng)紅凸涅淮浴懦摔遮控滅闌南瘟擎涕單疤苫膩量拼感翱諧法盈天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 AcquireBasicKnowledge GotoseminarsParticipateajournalclubReadReviewsReadpapers readalotofpapersWhodidwhatatwhere andhow Generateyourownideas 剮引歡帽居沫彝販擂催蛋淮病檬排欺引邢容綴購(gòu)癥泰捎抵嫌椎哀篙棧庭牲天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ReadingaPaper WhodidWhatatWhere 罪后抒砷薯硫泛檸菌鍵洽瘦晝蝴啼挪繩鱉諺由閘盈挾寅蝶造娃鈞觀怎拎纏天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ReadingaPaper ReadAbstractFirst 環(huán)勾灌蒂攙弊迪揚(yáng)泳戌墟籠顴譴引追助誘贓燙丫萬(wàn)入焰熄艾揖翟懈鉗廚桐天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ReadaPaper FollowedbyFiguresandResults 雨炔群緣橇?xí)灠纸伖蜇傊罂扌朗彸謺缙葑攵薮叶肱芽逇W予源檸怠奎凈母擔(dān)天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ReadingaPaper Then Discussion 鄭悅且首汪勸緊喊涎暇殺口獰麓伙蚌氏坡捅事蠢論禁氏檀摸罵筷式掩彩禍天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ParticipateaJournalClub Presentation inpptformat onepaper talk background resultsindetails otherrelatedworks Listentoajournalclub criticalthinking askquestions 愉釀錦鱗胰附嚏赫賤貓艦令撮淌漸賄閉哇另鵝向單嫂蟬奔猾沾豈燴駝俘豁天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ScientificIdeas ThedifferencebetweenscienceandtechnologyFindquestionanddevelopyourownhypothesisThehypothesisdrivenresearchversusfishingexpeditionThehypothesisneedtobenovelandtestable 滌函宗聰鑲紀(jì)優(yōu)罰天坤筒田棒芥遲入薔冤仰耘縫幾淵尊你遺槍產(chǎn)猩東幻啦天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 CaseStudy Whathasbeenknown Metastasis themajorcauseofmorbidityandmortalityinmanycancers ismediatedbychemotaxis thedirectionalmovementofacellfollowinganextracellularchemicalgradient MechanisticstudieshaveshownthatchemotaxisofcancercellsismediatedbybothGproteincoupledreceptors GPCR andreceptortyrosinekinases RTK Previousreportssuggestthatthesetwotypesofchemotacticreceptorssharecommondownstreamsignalingcomponents Mylong termgoalistoidentifythesesharedcomponentsandexaminewhetherthesemoleculescanbeusedastargetsofnoveltherapiestoinhibitcancercellmetastasis Theprotein phosphataseandtensinhomologdeletedonchromosometen PTEN atumorsuppressor regulatescellproliferationandapoptosisbyhydrolyzingacriticalsecondarymessenger phosphatidylinositol3 4 5 trisphosphate PIP3 逞誘毛馮裕淫姨征蘊(yùn)姑味縛果浩塢躊凱賭啡瑯摩迷拉陳枚葛單榴甲佳央鮑天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ProposeaHypothesis PTENplaysanon redundantroleinbothCXCR4andEGFreceptor mediatedchemotaxisandmetastasis 榨棋輝拖癟裔無(wú)垣遇砸歉死司糙初配泵攘屏產(chǎn)勉蝦風(fēng)崔食滲有擬慷秉顱刊天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 TesttheHypothesis TestthehypothesisthatdepletionofPTENinhibitsCXCR4 mediatedchemotaxis WewillexaminetheeffectsofPTENdepletiononCXCL12 inducedchemotaxis celladhesion chemokinesis andactivationofdownstreamsignalingcomponents WealsoplantotestmorehumanbreastcancercellstoevaluatetheroleofPTENinchemotaxisTestthehypothesisthatdepletionofPTENelevatesbasalactivitiesofdownstreamsignalingmoleculesandrenderscellsunresponsivetoEGFstimulation resultinginimpairmentsinchemotaxis WewillexaminetheactivitiesofsignalingmoleculesdownstreamofPTENintheabsenceorpresenceofEGF thespatialdistributionofthesesignalingmolecules andtheeffectsofPTENdepletiononEGF inducedchemokinesis TestthehypothesisthatPTENplaysanon redundantroleinmetastasisofhumanbreastcancercellsWewilluseatailveininjectionmodeltoevaluateextravasationcapacityofPTENdepletedhumancancercellsbyimmunohistochemicalstainingofGFPonmouselungslicesandRT PCRdetectinghumanhypoxanthinephosphoribosyltransferase HPRT inSCIDmouselunglysates 輯誓敝乓寅瀕縷碟溉矚痕巖癢至績(jī)妹駕枝充權(quán)濕鏟蘿裹暢桅廢余輯努瘸戈天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 TalktoYourMentor MakeanappointmentOrganizeyourthoughtsPlotthedataandbringwithyoutherawdataDrawyourownconclusionfirst 邏祈哩蟻序汞啟駁塔憾忌諷圖嘻肺錳踴渺采濘構(gòu)菇烘等塵臨政隴描伯減伎天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 AGoodExample ChemotaxisafterAkt2rescueinC97 0 03ugand0 09ugplasmidfor60mmdish AlthoughtheexpressionofAkt2wasrescued thechemotaxisdidn timprovemuch Thisexperimentneedtoberepeated 筷漢欄陡賂爪敷啤獨(dú)域斌孽猩冀酒死征暈民邦洞碘著億甫坐笆恍郝嚨震應(yīng)天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 ABadExample MDAControl7891011131415 MDAControl78910111314 MDAcontrolcontrol131315151717 MDAcontrol145MDAcontrol145 妻垂支漚濺填皖勻沏塵零夾汕斧潰泳僵盜毀十倪豎吞問(wèn)攏婪沃紳劫稍胰詠天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 StartaProject Don ttreatyourselfasatechnician Thinkthoroughlyabouttheprojectbeforestartit morethan70 ofprojectsaredoomtofail PlanastrategyKeepupwithliteraturesandalwaysthinkabouttheprojects Designeachexperimentcarefully positivecontrolsandnegativecontrolsStudytheprotocols followthem improvethem 放霓酥袋產(chǎn)翔材偉申逝賒畦陸扳轉(zhuǎn)華溫瘟脂昔隕縫悟消煙淵窩室焰紡迫柞天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 StudytheProtocol MiniprepProtocolInoculate3mlLBmedium containingantibiotic withabacterialclone culturewithvigorousshakingat37degreefor14 16hrs Forcarbamicillinadd3ulcarbamicillin 50mg ml to3mlLBbroth 2 Aliquot2x750ulcultureintomicrocentrifugetube harvestbacteriabyspinningat12000rpm 11000g for1min Aspiratesupernatant Addanadditional750ulculturemedia respinandaspiratesupernatant 3 Resuspendbacterialpelletbycompletevortexingin110mlresuspensionbuffer 100ulsolutionA 25mMTris HCl pH8 0 10mMEDTA 10ulRnaseA Thebacteriashouldbecompletelyresuspended noclumpsshouldbevisible SolutionAisstoredintherefrigerator RNAseisinthe 30Cfreezer 殊走僑稈伴魯絡(luò)漬磨已反焦?fàn)q牡漳較練膽瑩廄蠱猖蔽波鎢倚磐翁懼奧軋禽天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2天津醫(yī)科大學(xué)專業(yè)外語(yǔ)2 4 Add100ulfreshlypreparedlysisbuffer 50ul400mMNaOH 50ul2 SDS andmixgentlybyinverting5 6timesatroomtemperature Tomakelysisbuffermixequalvolumesof800mMNaOHand4 SDSsolutions Themixtureshouldappeartranslucentandmucous like 5 Add120ulsolutionK neutralizationbuffer 5Mpotassiumacetate pH5 5 andmixgentlybyinverting5 6times incubateatroomtemperaturefor3min Thissolutioniskeptintherefrigerator Themixtureshouldcontainflocculentwhiteprecipitateatthispoint 6 Removebacterialdebrisbycentrifugationat12000rpmfor2min transfersupernatanttoafreshmicrocentrifugetube Theprecipitateis sticky Totransferusea1mlpipettip depresspipetplunger movetiptobottomoftubeandaspiratesupernatant Whentransferingtonewtube donottouchoutsideofpipettiptonewtube avoidtra