分析化學(xué)紫外可見分光光度法zwkjC

1、123續(xù)前4續(xù)前min%11maxmax，shcmE5續(xù)前45. 315. 388. 170. 155036127836155036127812AAAAVB，三處最大吸收，定性鑒別：藥典規(guī)定6續(xù)前7續(xù)前%06. 01002101 . 1%/101 . 1100/101 . 1143505. 0334%11腎上腺酮mlmgmlglEACcm8lECA定量依據(jù)：9續(xù)前要求單色光前提：iECAmLgCigW 求含樣過程：已知稀釋)/(/)(lEAmLgCi100/lALmolCi/或%100%樣CCii10練習(xí)mLgmLglEAC/0 .20100/00200. 01207414. 011練習(xí)mLg

2、mLgCi/1045. 2100/1045. 21207507. 053mLgC/1050. 2100101001050. 252樣%0 .98%1001050. 21045. 2%100%5512樣CCBi12練習(xí)%)0 .764(591. 01001025000.20367255iEAmLmLmLmgmlmLHCL求已知測移取稀至樣品稀至吡啶 mLgmLglEACi/1092. 7100/1092. 7764591. 064%0 .99%10025010100100 . 21092. 7%100%26樣CCii13條件前提：固定儀器和測定CACKA樣樣同上條件固定條件查得測定樣品曲線分別測

3、定配制標準系列過程：CAACA標樣標樣標樣標樣AACCAACC14示例mLmgmLmLmLmg250 . 32550/200. 0樣品標樣分別移取15續(xù)前標樣與樣品濃度相近；截距為固定儀器和測定條件；前提：0標樣和分別測定一定過程：AASiSiAACCsCiCCSiSiAAmm%100%mmii16練習(xí)mLgCCii/1 .98518. 0508. 01000100000.25105 . 2%1 .98%100%12標示量標示量iCB17練習(xí)1207508. 01050. 2iiClEACmLgCi/1 .98%1 .98%100%12標示量標示量iCB18續(xù)前cbaAAAA總定量依據(jù)：aaa

4、aaaEACCEA1111由bbbbbbEACCEA2222由bbaaAEAE222111；測定；測定過程：19續(xù)前babaaaAEEAE2222111；和測定；測定過程：aaaaaaEACCEA1111由bbaababaCECEAAA22222由baababECEAC22220續(xù)前21babababaAEEAEE22221111；和測定；和測定bbaababaCECEAAA11111bbaababaCECEAAA22222bababbabbaaEEEEEAEAC12211221babaabaababEEEEEAEAC1221211222babababaAAAAAA222111babbaaba

5、babaAAAAAAAAA)()(212121aaAAa2121和的等吸收點選bbbbbbbbaCECEEAAA)(2121bbabbbabEAEEAC2123續(xù)前bbAAb 2 1 2 1和的等吸收點選aaaaaaabaCECEEAAA)(21 2 1abaaabaaEAEEAC 2 12425續(xù)前 倍率因子令KAAbb21)()()()(1212112212aabbababbababaAKAAKAAAAAKAKAAbA1bA2aaaaabaCEKEAKAA)(1212的干擾消除了bCAaba26練習(xí)9 .927%11cmEmLgmLgCi/10900. 4100/10900. 419 .927463. 064mLgC/10000. 55052001000.1063樣%0 .98%10010000. 510900. 4%100%66樣咖啡酸CCi5052001稀釋因子注：27練習(xí)%11cmE12000 .100120001010%11MEcmmLgmLglETlEACi/10165. 3100/10166.