pProEX-HTc大腸桿菌表達(dá)載體說(shuō)明

1、ProEX HTc編號(hào)名稱北京華越洋 VECT-370pProEX HTcpProEXHTc 載體基本信息別名:pProEX HTc, pProEX HTc質(zhì)粒類型:大腸桿菌蛋白表達(dá)表達(dá)水平:高克隆方法:多克隆位點(diǎn)，限制性內(nèi)切酶載體大小:4779bp5 測(cè)序引物序列:M13/pUCReverse: AGCGGATAACAATTTCACACAGG(Invitrogen)3 測(cè)序引物序列:pTrcHis Reverse: GATTTAATCTGTATCAGG (Invitrogen)載體標(biāo)簽:N-His, N-TEV 蛋白酶切位點(diǎn)載體抗性:氨芐備注:Hosts: E.coli. Related v

2、ectors: pBR322.穩(wěn)定性:瞬時(shí)表達(dá) Transient組成型/誘導(dǎo)型:誘導(dǎo)型病毒/非病毒:非病毒pProEXHTc 載體質(zhì)粒圖譜和多克隆位點(diǎn)信息pProEXHTc 載體簡(jiǎn)介pProEX HT 系列原核表達(dá)系統(tǒng)是用來(lái)在大腸桿菌中表達(dá)外源蛋白的載體。使用該載體表達(dá)的目的蛋白含有 6 個(gè) His 純化標(biāo)簽。目的基因可以克隆到載體的多克隆位點(diǎn)處，可以選擇的載體有 pProEX HTa, b, c.蛋白表達(dá)后，His 純化標(biāo)簽就位于目的蛋白的 N 端，同時(shí) His 純化標(biāo)簽的使用可以用于蛋白純化。載體上同時(shí)含有 rTEV 蛋白酶識(shí)別位點(diǎn)，可以使用 rTEV 蛋白酶進(jìn)行酶切，從而去除融合的 H

3、is 標(biāo)簽。pProEXHTc 載體序列ORIGIN1 GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC61 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC121GCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTC181TGAAATGAGCTGTTGACAATTAATCATCCGGTCCGTATAATCTGTGGAATTGTGAGCGGA241TAACAATTTC

4、ACACAGGAAACAGACCATGTCGTACTACCATCACCATCACCATCACGATT301ACGATATCCCAACGACCGAAAACCTGTATTTTCAGGGCGCCATGGGGATCCGGAATTCAA361AGGCCTACGTCGACGAGCTCACTAGTCGCGGCCGCTTTCGAATCTAGAGCCTGCAGTCTC421GAGGCATGCGGTACCAAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATA481CAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTA

5、GC541GCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGT601AGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGC661TCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAG721TAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCG781GGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAG

6、AAGGCCATCCTGACGGA841TGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATG901TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT961ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT1021GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA1081CGAGTGGGTTACATCGAACTGGATCT

7、CAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC1141GAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC1201CGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG1261GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA1321TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATC1381GGAGGA

8、CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT1441GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG1501CCTACAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT1561TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC1621TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGA

9、GCGTGGGTCT1681CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC1741ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC1801TCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGAT1861TTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATG1921ACCAAAATCCCTTAACGTGAGTTTTCGTTC

10、CACTGAGCGTCAGACCCCGTAGAAAAGATC1981AAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAA2041CCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAG2101GTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTA2161GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTA2221CCAGTGGCTG

11、CTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAG2281TTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTG2341GAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACG2401CTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAG2461CGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGG

12、TTTCGC2521CACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA2581AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG2641TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCT2701GATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAA2761GAGCGCCTGATGCGGTATTTTCTCCTTACGCATC

13、TGTGCGGTATTTCACACCGCATAATT2821TTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAA2881ATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCA2941GTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACC3001GTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCG3061AGGTGCCGTAAAGC

14、ACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGG3121GGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGG3181GCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCG3241CCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCTATGGTGCACTCTCAGTACAATC3301TGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCAATATTGCTACGTGACTGGTA

15、CA3361CTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTG3421ACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCT3481CCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGA3541TCAATTCGCGCGCGAAGGCGAAGCGGCATGCATTTACGTTGACACCATCGAATGGTGCAA3601AACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGA

16、GTCAATTCAGGGTGGTGAATGT3661GAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTC3721CCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGC3781GATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTC3841GTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGC3901GGCGATTAAATCTCGCGC

17、CGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACG3961AAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGG4021GCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCAC4081TAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTT4141CTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCA42

18、01AATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTG4261GCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAG4321 TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC4381 GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG4441 GCTGCGCGTT GGTGCGGATA TCTCGGT

19、AGT GGGATACGAC GATACCGAAG ACAGCTCATG4501 TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT4561 GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT4621 CTCACTGGTG AAAAGAAAAA CCACCCTGGC ACCCAATACG CAAACCGCCT CTCCCCGCGC4681 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACT

20、GGAAA GCGGGCAGTG4741 AGCGCAACGC AATTAATGTG AGTTAGCGCG AATTGATCT/其他大腸桿菌表達(dá)載體： pBV221pDsRed-Express2 pCold TFpCold IptdTomato pBV220pCold IVpE-SUMOpET-52b(+)pCold-GST pCold III pCold-ProS2pAmCyan pColdS-SUMO pCold IIpBAD102/D-TOPOpBAD202/D-TOPOpACYC184pBAD/Thio-TOPOpBad/Myc-His CpBad/Myc-His BpBad/Myc-

21、His ApBad/His CpBad/His BpBad/His ApBAD-TOPOpET-23b(+)pET-23a(+)pET-23c(+)pET-23(+)pET-12b(+)pET-12c(+)pET-12a(+)pET-11b(+)pET-11a(+)pET-11c(+)pBad24pQE-82LpQE-81LpQE-80LpQE-32pQE-9pQE-16pQE-31pQE-60pQE-70pQE-40pET-51b(+)pET-50b(+)pET-49b(+)pET-48b(+)pET-47b(+)pET-26b(+)pET-32a(+)pET-21b(+)pET-22b(+

22、)pET-14bpET-16bpET-15bpET-19bpET-20b(+)pET-21d(+)pET-21c(+)pET-21b(+)pET-21a(+)pET-24a(+)pET-24d(+)pET-25b(+)pET-27b(+)pET-28a(+)pET-30a(+)pET-42a(+)pET-43.1c(+)pET-43.1b(+)pET-43.1a(+)pET-44a(+)pET-44c(+)pET-46 EK/LICpET-37b(+)pTrcHis2 CpTrcHis2 BpTrcHis2 ApET303/CT-HispET302/NT-HispRSET-CFPpRSET-E

23、mGFPpRSET-BFPpGFPuvpET300/NT-DESTpET301/CT-DESTpGEM-TpBad43pGEX-4T-3pGEX-5X-2pBlueScript SK(+)pG-Tf2pG-KJE8pGro7pET-SUMOpSE380pET-17bpET102/D-TOPOpCDFDuet-1pMAL-p5xpTf16pET-28c(+)pBluescript II SK(+)pET-30b(+)pSUMOpProEX HTcpProEX HTbpProEX HTapKD3pKD13pKD46pTYB1pTYB2pTWIN2pBluescript II KS(-)pTYB12pMAL-p5epACYCDuet-1pEGM-11ZF(+)pEGM-7ZF(+)PinPoint Xa-3PinPoint Xa-2PinPoint Xa-1pSP73