Chitosan-oligosaccharide-COS-DataSheet-生命科學試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEChitosan oligosaccharideCat. No.: HY-112108CAS No.: 148411-57-8Synonyms: COS作靶點: AMPK作通路: Epigenetics; PI3K/Akt/mTOR儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 50 mg/mL (Need ultrasonic)

2、DMSO : 50 mg/mL (Need ultrasonic)請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內實驗 請根據(jù)您的實驗動物和給藥式選擇適當?shù)娜芙獍?，配制前請先配制澄清的儲備液，再依次添加助溶?為保證實驗結果的可靠性，體內實驗的作液，建議您現(xiàn)現(xiàn)配，當天使；澄清的儲備液可以根據(jù)儲存條件，適當保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (Infinity mM); Cl

3、ear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (Infinity mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (Infinity mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Chitosan oligosaccharide (COS)種 由 -(14) 糖苷鍵連接的 D-葡糖胺的寡聚物。Chitosanoligosaccharid

4、e (COS) 激活 AMPK 并抑制包括 NF-B 和 MAPK 途徑的炎癥信號途徑。IC50 & Target AMPK 11/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE體外研究 Chitosan oligosaccharide (COS) represents a class of natural polymers that holds therapeutic promise inseveral diseases due to not only its physiochemical properties including water-

5、solubility and low viscosity butalso its favorable pharmacological properties including good pharmacokinetics and safety profiles anddiverse beneficial biological activities. Activation of AMPK and inhibition of inflammatory signaling pathwaysincluding NF-B and MAPK pathways are recognized as major

6、mechanisms responsible for several effects ofChitosan oligosaccharide (COS) including anti-inflammation, anti-cancer, and anti-diabetes. COS caninterrupt cancer progression at multiple stages by modulating several signaling proteins/pathways includingNF-B, AMPK, mTOR, caspase-3, CD147, MMP-2, MMP-9,

7、 and VEGF. In vitro experiments havedemonstrated that Chitosan oligosaccharide (COS) induced the death of several cancer cell types includingascites, bladder cancer, prostate cancer, lung cancer, liver cancer, leukemia, cervical cancer and colorectalcancer. The values of IC50 of Chitosan oligosaccha

8、ride (COS) in inducing cytotoxicity are 25 g/mL-50 g/mL depending on types of cancer cells 1.體內研究 The oral administration of Chitosan oligosaccharide (16 mg/kg/day) suppresses the production of theproinflammatory cytokines involved in allergic reactions, i.e., IL-4, IL-13 and TNF-, in the lung tissu

9、es andbronchoalveolar lavage fluid of the mice. Last, an anti-inflammatory effect of Chitosan oligosaccharide (COS)on lymphocyte activation has been documented in a rat model of autoimmune anterior uveitis induced byimmunization with bovine melanin-associated antigen 1. Chitosan oligosaccharide (COS

10、) inhibits UV-induced macroscopic appearance in mice skin. Compared with healthy dorsal skin with smoothness andsome shallow wrinkles of hairless mice in normal control group, UV exposure for 10 weeks triggers skinerythema, dry, thickening, sagging and coarse wrinkles, and even leathery appearance a

11、nd slight flesh-colored lesion in the model mice, the visual score of which is markedly higher than that of the normal controlgroup (p 2.PROTOCOLAnimal Mice 2Administration 2 Seven-week-old female hairless BALB/c mice (n=12), weighing approximately 16 g, are used. After a week ofacclimation, the hai

12、rless mice are randomly divided into five groups of 6 mice per group: Normal controlgroup (without UV irradiation but with double-distilled water treatment); Model group (UV irradiation withdouble-distilled water treatment); COS-L group, COS-M group and COS-H group (UV irradiation with 50mg/mL, 100

13、mg/mL and 200 mg/mL COS treatment, respectively). For mice used for topical application, 100L are applied to each mouse dorsal skin every time after UV-radiation. The dorsal treated skin area of miceis carefully wiped with soft absorbent cotton soaked in distilled water, and then wiped with dry cott

14、on beforeeach UV-radiation to remove any remaining Chitosan oligosaccharide (COS) 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Muanprasat C, et al. Chitosan oligosaccharide: Biological activities and potential therapeutic applications. Pharmacol Ther. 2017Feb;170:80-972. Kong SZ, et al. Anti-photoaging effects of chitosan oligosaccharide in ultraviolet-irradiated hairless mouse skin. Exp Gerontol. 2018Mar;103:27-34.2/3 Master of Small Molecules