Liothyronine-Triiodothyronine-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemELiothyronineCat. No.: HY-A0070ACAS No.: 6893-02-3Synonyms: Triiodothyronine; 3,3,5-Triiodo-L-thyronine; T3分式: CHINO分量: 650.97作靶點: Thyroid Hormone Receptor作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C

2、1 month溶解性數(shù)據(jù)體外實驗 DMSO : 6.9 mg/mL (10.60 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.5362 mL 7.6808 mL 15.3617 mL5 mM 0.3072 mL 1.5362 mL 3.0723 mL10 mM 0.1536 mL 0.7681 mL 1.5362 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性

3、 Liothyronine種有效的甲狀腺激素，可以結(jié)合并激活 1 甲狀腺激素受體 (TR1)。IC50 & Target Human Endogenous Metabolite體外研究Liothyronine (T3, 100 nM) stimulates the proliferation of hepatocarcinema cells in which TR1 is1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEoverexpressed 1. Liothyronine binds to human 1 thyroid hormone r

4、eceptor (hTR1), and change itsconformation. Liothyronine promotes growth, induces differentiation and regualtes metabolic effects 2.PROTOCOLCell Assay 1 Thyroid hormone depleted (Td) serum is prepared. The growth of hepatocarcinoma cells in methylcellulose isperformed. To determine the effect of Lio

5、thyronine (T3) on the growth of cells, cells are plated at a density of3 l04 cells/60 mm dish on day 0, and incubated in medium containing 5% regular serum, 5% Td or 5% Tdand 100 nM T3. The colony formation in methylcellulose is scored 3 weeks after initial plating 1.MCE has not independently confir

6、med the accuracy of these methods. They are for reference only.REFERENCES1. Lin KH, et al. Stimulation of proliferation by 3,3,5-triiodo-L-thyronine in poorly differentiated human hepatocarcinoma cellsoverexpressing beta 1 thyroid hormone receptor. Cancer Lett. 1994 Oct 14;85(2):189-94.2. Bhat MK, et al. Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3,5-triiodo-L-thyronine.Biochem Biophys Res Commun. 1993 Aug 31;195(1):385-92.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use onl