常用凝膠參數(shù)

1、FpgFpg常用凝膠技術(shù)參數(shù)1葡聚糖凝膠某些技術(shù)數(shù)據(jù)種類干顆粒直徑（ ）分子量分級(jí)范圍床體積毫升 /克干分子篩得水值溶脹最少平衡時(shí)間（ h）柱頭壓力 （ kPa） （2.5cm 直徑柱）肽及球形 蛋白質(zhì)葡聚糖（線性分子）室 溫沸 水 浴Sephadex G-1040120 700 7002 31.0 0.131Sephadex G-1040120 150015002.53.51.5 3.531Sephadex G-25粗級(jí)100 300( 5 100 目 )中級(jí)50 150( 100 200目)1,0005,0001005,0004 61.5 0.262細(xì)級(jí)20800( 200400 目 )超

2、細(xì)10 40Sephadex G-50粗級(jí)100 200中級(jí)50 1501,500500細(xì)級(jí)20 8030,00010,0009 115.0 0.362超細(xì)10 40Sephadex G-7540 1203,0001,000超細(xì)10 4070,000950,00012157.5 0.52433.92 15.86SephadexG-10040 1204,0001,000超細(xì)10 401,500,000 150,000152010.0 1.04852.359.41SephadexG-15040 1205,0001,0002030超細(xì)10 40400,000150,000182215.0 1.572

3、50.883.53SephadexG-20040 12050001000304010 40800,000200,000202520.0 2.07250.391.572. 聚丙烯酰胺凝膠技術(shù)數(shù)據(jù)型號(hào)排阻下限（ 分子量）分級(jí)分離范圍（ 分子量）膨脹后床體積 （ml/g 干凝 膠）膨脹所需最少時(shí)間 （ 室溫 ,h）Bio-gel-P-21,600200 2,0003.82 4Bio-gel-P-43,600500 4,0005.82 4Bio-gel-P-64,6001,000 5,0008.82 4Bio-gel-P-1010,0005,000 17,00012.42 4Bio-gel-P-303

4、0,00020,000 50,00014.910 12Bio-gel-P-6060,00030,000 70,00019.010 12Bio-gel-P-100 100,00040,000100,00019.024Bio-gel-P-150 150,00050,000150,00024.024Bio-gel-P-200 200,00080,000200,00034.048Bio-gel-P-300 300,000100 400,00040.0483瓊脂糖凝膠技術(shù)數(shù)據(jù)型號(hào)瓊脂糖含量%（ W/W）排阻下限（分子量）分級(jí)分離范圍（分子量）生產(chǎn)廠家Sepharose 4B40.3 106 3 106P

5、harmaciaSepharose 2B22 106 25 106Sagavac 10102.5 1051 104 2.5 105SeravacSagavac 887 1052.5 104 7 105Sagavac 662 1065 104 2 106Sagavac 4415 1062 105 15 106Sagavac 22150 1065 105 15 107Bio-gel A-0.5M100.5 1061 104 0.5 106Bio-gel A-1.5M81.5 1061 104 1.5 106Bio-gel A-5M65 1061 104 5 106Bio-gel A-15M415

6、1064 104 15 106Bio-RadBio-gel A-50M250 1061 105 50 106Bio-gel A-150M1150 1061 106 150 1064各處凝膠所允許最大操作壓凝膠最大靜水壓（kPa）SephadexG-109.8G-159.8G-259.8G-509.8G-754.95瓊脂糖凝膠濃度與線性DNA分辨范圍凝膠濃度（%）線性DNA長(zhǎng)度（bp）0.51000 300000.7800 120001.0500 100001.2400 70001.5200 30002.050 2000在變性聚丙烯酰胺凝膠中遷移速度6 染料凝膠濃度（%）溴酚藍(lán)二甲苯青FF5 0

7、35bp140bp6 026bp106bp8 019bp75bp10 012bp55bp20 08bp28bp7染料在非變性聚丙烯酰胺凝膠中遷移速度凝膠濃度（%）溴酚藍(lán)二甲苯青FF3.5100bp460bp5.065bp260bp8.045bp160bp12.020bp70bp15.015bp50bp20.012bp45bp七、氨基酸特性八、遺傳密碼UCAG密碼子UUUPheUCUSerUAUT yrUGUG ysUUUCPheUCCSerUACT yrUGGG ysCUUUALeuUCASerUAA終止 （赭石）UGA終止（乳 白）A第三位（ 3UUGLeuUGGSerUA終止 （琥珀）UG

8、GT rpG端）CUULeuCCUProCAUH isCGAA rgUCCUCLeuCCCProCACH isCGCA rgC密碼子第二位5 密碼 子第一位 （端）CUALeuCCAProCAAG lnCGAA rgACUGLeuCCGProCAGG lnCGCA rgGAUUIleACUThrAAUA snAGUS erUAUCIleACCThrAACA snAGCS erCAAUAIleACAThrAAA LysAGAA rgAAUGMetACGThrAAGL ysAGCA rgGGUUValGCUAlaGAUA spGGUG lyUGUCValGCCAlaGACA spGGCG lyCG

9、GUAValGCAAlaGAAG luGGAG lyAGUGValGCGAlaGAGG luGGGG lyG九、常用酸堿技術(shù)參數(shù)1常見市售酸堿濃度溶質(zhì)分子式分子量mol/L gL重量（%）比重配制 mol/L 溶液 加入量 （ ml/L ）冰乙酸CH3COOH60.0517.40 1045 99.51.0507.5乙酸60.056.27376361.04559.5甲酸HCOOH46.0223.40 1080 901.2002.7鹽酸HCl36.5011.60 424361.1806.22.90105101.050 344.8硝酸HNO363.0215.99 1008 711.420 62.51

10、4.90 938671.400 67.113.3037611.370 75.2高氯酸HclO3100.50 11.65 1172 701.670 85.89.20923601.54008.7磷酸H2PO480.0018.10 1445 851.700 55.2硫酸H2P O498.1018.00 1776 961.840 55.6氫氧化銨NH4OH35.0014.8051280.8987.6氫氧化鉀KOH56.1013.5057501.5204.11.94109101.090 515.5氫氧化鈉NaOH40.0019.1063501.530 52.42.75111101.110 363.42各種濃度酸堿貯存液近似pH值溶質(zhì)1Na0.1Na0.01Na0.0