MRL-494-hydrochloride-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEMRL-494hydrochlorideCat.No.:HY-128773A分?式:C??H??ClFN??O?分?量:659.12作?靶點(diǎn):Bacterial作?通路:Anti-infection儲(chǔ)存?式:4°C,protectfromlight,storedundernitrogen*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light,storedundernitrogen)溶解性數(shù)據(jù)體外實(shí)驗(yàn)H2O:110mg/mL(166.89mM;Needultrasonic)DMSO:100mg/mL(151.72mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.5172mL7.5859mL15.1717mL5mM0.3034mL1.5172mL3.0343mL10mM0.1517mL0.7586mL1.5172mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(protectfromlight,storedundernitrogen)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：PBSSolubility:25mg/mL(37.93mM);Clearsolution;Needultrasonicandwarming1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性MRL-494hydrochloride?種抗劑，?種BamA的抑制劑，具有抗外排和外膜滲透性。MRL-494hydrochloride可抑制?蘭?陽性細(xì)(??葡萄球COL的MIC值為12.5μM)和?蘭?性細(xì)(?腸桿的MIC值為25μM)。IC50&Targetβ-barrelassemblymachineA(BamA)[1]體外研究MRL-494lethallydisruptsthecytoplasmicmembrane.MRL-494inhibitsOMproteins(OMPs)biogenesisfromoutsidetheoutermembrane(OM)bytargetingBamA.MRL-494exhibitsstronganti-microbialpropertiesagainstbothGram-positiveandGram-negativebacteria.TheMICvaluesofMRL-494againstE.coli(WT),E.coli(ΔtolC),E.coli(ΔtolCenvA101),K.pneumonia,A.baumannii(WT),A.baumannii(ΔlpxC),P.aeruginosa(effluxdeficient),P.aeruginosa(WT),Staphylococcusaureus(methicillin-resistant)andBacillussubtilisrpoB18are25μM,25μM,25μM,100μM,200μM,200μM,100μM,100μM,12.5μMand25μM,respectively[1].REFERENCES[1].HartEM,Asmall-moleculeinhibitorofBamAimpervioustoeffluxandtheoutermembranepermeabilitybarrier.ProcNatlAcadSciUSA.2019Oct22;116(43):21748-21757.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly