黃瓜白粉病抗性基因挖掘結論及參考文獻,農藝學論文

黃瓜白粉病抗性基因挖掘結論及以下為參考文獻,農藝學論文本篇論文目錄導航：【題目】【第一章】【第二章】【第三章】【結論/以下為參考文獻】黃瓜白粉病抗性基因挖掘結論及以下為參考文獻第四章全文結論1．本研究利用高抗白粉病種質PI200815與感病親本931進行雜交自交，構建遺傳群體，利用苗期人工噴霧接種，對雙親和遺傳群體進行抗病性鑒定，利用病情指數(shù)對白粉病抗性進行遺傳分析，結果表示清楚，F(xiàn)2和F2:3后代的病情指數(shù)呈連續(xù)近正態(tài)分布，表示清楚PI200815的白粉病抗性是由多基因控制的數(shù)量性狀。2．利用F2遺傳群體構建了一張包含129個SSR分子標記的連鎖圖譜，覆蓋基因組長度857.9cM，標記間平均遺傳距離為6.65cM。3．利用構建的遺傳圖譜，結合三季度的抗病性鑒定結果，對PI200815中的抗病基因進行了QTL分析，2020年春，檢測到兩個QTL位點：pmQTL1.1和pmQTL6.1，LOD值分別為4.6和3.3，表型解釋率分別為10%和7%。2020年春，只檢測到一個QTL位點pmQTL1.1，LOD值到達了15.3，表型解釋率到達了37.9%。2020年秋，同樣只檢測到pmQTL1.1，LOD值為4.9，表型解釋率到達了21.6%。三個季度共同檢測到一個QTL位點pmQTL1.1，位于黃瓜1號染色體標記SSR03860-SSR14445之間，物理距離為689Kb。4．針對白粉病抗性主效QTL定位區(qū)域進行候選基因預測，發(fā)現(xiàn)目的區(qū)域有95個預測基因，功能與抗病相關的基因有10個。包含2個NBS類抗病蛋白，5個抵御素類似基因，2個逆境調控蛋白，和1個調控植物程序性死亡基因。5．利用已完成全基因組重測序的黃瓜核心種質，對白粉病抗性基因進行了三個季度的GWAS分析，檢測到6個信號位點〔pmG1.1，pmG2.1，pmG4.1，pmG5.1，pmG5.2和pmG5.3〕，華而不實pmG2.1、pmG5.2和pmG5.3在三季度中都被監(jiān)測到，同時將GWAS結果與前人研究比擬，發(fā)現(xiàn)華而不實有三個位點〔pmG1.1，pmG5.2，pmG5.3〕與前人QTL定位結果吻合。以下為參考文獻1.程嘉琪，沈鏑，李錫香，宋江萍，王海平，邱楊.黃瓜核心種質對白粉病的田間抗性評價.中國蔬菜，2018，20：15-192.董玉琛，曹永生，張學勇，劉三才，王蘭芬，游光霞，龐斌雙，李立會，賈繼增.中國普通小麥初選核心種質的產生.植物遺傳資源學報，2003，4(1)1-83.馮東昕，李寶棟.主要瓜類作物抗白粉病育種研究進展.中國蔬菜，1996(1)：55-59．4.劉龍洲，蔡潤，袁曉君，何歡樂，潘俊松.黃瓜抗白粉病QTL分子標記定位.中國科學,2008，38(9)：851-8565.劉龍洲，何歡樂，潘俊松，杜輝，蔡潤.120份黃瓜種質白粉病(Sphaerothecafuliginea)抗性鑒定.種子，2008，27(2)：60-626.劉苗苗.黃瓜霜霉病、白粉病抗病基因的QTL定位[碩士學位論文].哈爾濱：東北農業(yè)大學，20187.劉苗苗，劉宏宇，顧興芳，張圣平，苗晗.黃瓜白粉病抗性遺傳規(guī)律及分子標記研究進展.中國蔬菜，2018，24：7-128.劉秀波，崔琦，崔崇士.瓜類白粉病抗性育種研究進展.東北農業(yè)大學學報，2005，36(6)：794-7989.呂淑珍，霍振榮，陳正武等.黃瓜霜霉病、白粉病抗性遺傳研究初報.見：李樹德.中國主要蔬菜抗病育種進展.北京：科學出版社，1995，436-43810.毛愛軍，張峰，張海英，張麗蓉，王永健.兩個黃瓜品種對白粉病的抗性遺傳分析.中國農學通報，2005，21(6)：302-30511.聶京濤，潘俊松，何歡樂，司龍亭，蔡潤.中國蔬菜.2018，10：45-4912.齊建建.黃瓜遺傳多樣性和人工馴化的分子基礎[博士學位論文].北京：中國農業(yè)科學院，202013.曲麗，秦智偉.黃瓜白粉病病原菌及抗病性研究進展.東北農業(yè)大學學報，2007，38〔6〕：835-84114.沈麗平.黃瓜白粉病抗性遺傳分析及相關QTL初步定位[碩士論文].揚州：揚州大學，201815.涂雨辰，田云，盧向陽，全基因組關聯(lián)分析在植物中的應用?；瘜W與生物工程，2020，30〔6〕：7-1016.王明.玉米抗絲黑穗病的全基因組關聯(lián)分析[博士學位論文].武漢：華中農業(yè)大學，202017.王建設，宋曙輝，孟淑春，陳貴林.兩個甜瓜品種對白粉病菌的抗性遺傳分析.華北農學報，2003，18〔2〕：63-6518.汪維鵬，倪坤儀，周國華.單核苷酸多態(tài)性檢測方式方法的研究進展.遺傳，2006，28〔1〕：117-12619.王振國.黃瓜白粉病抗性基因遺傳規(guī)律和相關分子標記的研究[碩士學位論文].哈爾濱：東北農業(yè)大學，200720.許啟新,陸世鈞等.1994.黃瓜霜霉病苗期抗性與成株期抗性相關性的研究.上海農業(yè)科技,(1):25~2521.張海英，王振國，毛愛軍，張峰，王永健，許勇.與黃瓜白粉病抗病基因嚴密連鎖的SSR分子標記.華北農學報，2008，23(6)：77-8022.張海英.黃瓜重要抗病基因的分子標記研究及遺傳圖譜的構建[博士學位論文].北京：中國農業(yè)科學學院，200623.張桂華，杜勝利，王鳴，馬德華.與黃瓜抗白粉病相關基因連鎖的AFLP標記的獲得.園藝學報，2004，31(2)：189-19224.張圣平，顧興芳，王燁，苗晗.十一五我們國家黃瓜遺傳育種研究展.中國蔬菜，2018，22：1-1025.張圣平，劉苗苗，苗晗，張素勤，楊宇紅，謝丙炎，顧興芳.黃瓜白粉病抗性基因的QTL定位.中國農業(yè)科學，2018，44(17)：3584-359326.張素勤，顧興芳，張圣平，鄒志榮.黃瓜白粉病抗性遺傳機制的研究.園藝學報，2005，32(5)：899-90127.張素勤，顧興芳，張圣平，鄒志榮.黃瓜對霜霉病和白粉病抗性的相關性研究.中國蔬菜，2007，9：9-1128.張素勤.黃瓜霜霉病和白粉病抗性遺傳機制及其AFLP分子標記研究[學位論文].楊凌:西北農林科技大學，200529.張艷菊，左紅波，曲麗，秦智偉，周秀艷.黑龍江省黃瓜白粉病病原鑒定.東北農業(yè)大學學報，2018，41〔4〕：20-2330.周益林，段霞瑜，盛寶欽.植物白粉病的化學防治進展.農藥學學報，2001，3〔2〕：12-1831.AndersonP.A.,LawrenceG.J.,MorrishB.C.,AyliffeM.A.,FinneganE.J.,EllisJ.G.InactivationoftheflaxrustresistancegeneMassociatedwithlossofarepeatedunitwithintheleucine-richrepeatcodingregion.Plantcell1997,9:641-651.32.AranzanaM.J.,KimS.,ZhaoK.,BakkerE.,HortonM.,JakobK.,ListerC.,MolitorJ.,ShindoC.,TangC.,ToomajianC.,TrawB.,ZhengH.,BergelsonJ.,DeanC.,MarjoramP.,NordborgM.Genome-WideAssociationMappinginArabidopsisIdentifiesPreviouslyKnownFloweringTimeandPathogenResistanceGenes.Plosgenetics,2005,1(5):e6033.AriteT,UmeharaM,IshikawaS,HanadaA.,MaekawaM.,YamaguchiS.,KyozukaJ.d14,Astrigolactione-in-sensitivemutantofrice,showsanacceleratedoutgrowthoftillers.PlantCellPhysiol,2018,50(8):1416-142434.AxtellM.J.,StaskawiczB.J.InitiationofRPS2-specifieddiseaseresistanceinArabidopsisiscoupledtotheAvrRpt2-directedeliminationofRIN4.Cell,2003,112:369-377.35.BarnesW.C.,EppsW.M.PowderymildewresistanceinSouthCarolinacucumbers.PlantDiseaseReport1956,40:1093.36.BlockC.C.,ReitsmaK.R.PowderymildewresistanceintheU.S.Nationalplantgermplasmsystemcucumbercollection.HortScience2005,40(2):416-42037.BrueggemanR.,RostoksN.,KudrnaD.,etal.Thebarleystemrust-resistancegeneRpg1isanoveldisease-resistancegenewithhomologytoreceptorkinases.ProcNatlAcadSciUSA.2002,99(14):9328-9333.38.BryanG.T.,WuK.S.,FarrallL.,JiaY.,FaulkK.N.,DonaldsonG.K.,TarchiniR.,ValentB.AsingleaminoaciddifferencedistinguishesresistantandsusceptibleallelesofthericeblastresistancegenePi-ta.PlantCell2000,12:2033-2046.39.BrueggemanR.,RostoksN.,KudmaD.,KilianA.,HanF.,ChenJ.,DrukaA.,SteffensonB.,KleinhofsA.Barleystemrust-resistancegeneRpglisanoveldisease-resistancegenewithhomologytoreceptorkinases.ProcNatlAcadSci,USA2002,99:9328-9333.40.BueschgesR.,HollricherK.,PanstrugaR.,SimonsG.,WolterM.,FrijtersA.,VanDaelenR.,vanderLeeT.,DiergaardeP.,GroenendijkJ.,TopschS,VosP.,SalaminiF.,Schulze-lefertP.ThebarleyMlogene:Anovelcontrolelementofplantpathogenresistance.Cell1997,88:695-705.41.CaiD.,KleineM.,KifleS.,JoachimH.J.,SandalN.N.,MarckerK.A.,Klein-LaxikhorstR.M.,SalentijnE.M.J.,LangeW.,StiekemaW.J.,WyssU.,GmndlerF.M.W.,JungC.Positionalcloningofagenefornematoderesistanceinsugarbeet.Science1997,275:832-834.42.DanglJ.L.,JonesJ.D.Plantpathogensandintegrateddefenceresponses^toinfection.Nature2001,411: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