黃瓜白粉病抗性基因挖掘結(jié)論及參考文獻(xiàn),農(nóng)藝學(xué)論文

黃瓜白粉病抗性基因挖掘結(jié)論及以下為參考文獻(xiàn),農(nóng)藝學(xué)論文本篇論文目錄導(dǎo)航：【題目】【第一章】【第二章】【第三章】【結(jié)論/以下為參考文獻(xiàn)】黃瓜白粉病抗性基因挖掘結(jié)論及以下為參考文獻(xiàn)第四章全文結(jié)論1．本研究利用高抗白粉病種質(zhì)PI200815與感病親本931進(jìn)行雜交自交，構(gòu)建遺傳群體，利用苗期人工噴霧接種，對(duì)雙親和遺傳群體進(jìn)行抗病性鑒定，利用病情指數(shù)對(duì)白粉病抗性進(jìn)行遺傳分析，結(jié)果表示清楚，F(xiàn)2和F2:3后代的病情指數(shù)呈連續(xù)近正態(tài)分布，表示清楚PI200815的白粉病抗性是由多基因控制的數(shù)量性狀。2．利用F2遺傳群體構(gòu)建了一張包含129個(gè)SSR分子標(biāo)記的連鎖圖譜，覆蓋基因組長(zhǎng)度857.9cM，標(biāo)記間平均遺傳距離為6.65cM。3．利用構(gòu)建的遺傳圖譜，結(jié)合三季度的抗病性鑒定結(jié)果，對(duì)PI200815中的抗病基因進(jìn)行了QTL分析，2020年春，檢測(cè)到兩個(gè)QTL位點(diǎn)：pmQTL1.1和pmQTL6.1，LOD值分別為4.6和3.3，表型解釋率分別為10%和7%。2020年春，只檢測(cè)到一個(gè)QTL位點(diǎn)pmQTL1.1，LOD值到達(dá)了15.3，表型解釋率到達(dá)了37.9%。2020年秋，同樣只檢測(cè)到pmQTL1.1，LOD值為4.9，表型解釋率到達(dá)了21.6%。三個(gè)季度共同檢測(cè)到一個(gè)QTL位點(diǎn)pmQTL1.1，位于黃瓜1號(hào)染色體標(biāo)記SSR03860-SSR14445之間，物理距離為689Kb。4．針對(duì)白粉病抗性主效QTL定位區(qū)域進(jìn)行候選基因預(yù)測(cè)，發(fā)現(xiàn)目的區(qū)域有95個(gè)預(yù)測(cè)基因，功能與抗病相關(guān)的基因有10個(gè)。包含2個(gè)NBS類抗病蛋白，5個(gè)抵御素類似基因，2個(gè)逆境調(diào)控蛋白，和1個(gè)調(diào)控植物程序性死亡基因。5．利用已完成全基因組重測(cè)序的黃瓜核心種質(zhì)，對(duì)白粉病抗性基因進(jìn)行了三個(gè)季度的GWAS分析，檢測(cè)到6個(gè)信號(hào)位點(diǎn)〔pmG1.1，pmG2.1，pmG4.1，pmG5.1，pmG5.2和pmG5.3〕，華而不實(shí)pmG2.1、pmG5.2和pmG5.3在三季度中都被監(jiān)測(cè)到，同時(shí)將GWAS結(jié)果與前人研究比擬，發(fā)現(xiàn)華而不實(shí)有三個(gè)位點(diǎn)〔pmG1.1，pmG5.2，pmG5.3〕與前人QTL定位結(jié)果吻合。以下為參考文獻(xiàn)1.程嘉琪，沈鏑，李錫香，宋江萍，王海平，邱楊.黃瓜核心種質(zhì)對(duì)白粉病的田間抗性評(píng)價(jià).中國(guó)蔬菜，2018，20：15-192.董玉琛，曹永生，張學(xué)勇，劉三才，王蘭芬，游光霞，龐斌雙，李立會(huì)，賈繼增.中國(guó)普通小麥初選核心種質(zhì)的產(chǎn)生.植物遺傳資源學(xué)報(bào)，2003，4(1)1-83.馮東昕，李寶棟.主要瓜類作物抗白粉病育種研究進(jìn)展.中國(guó)蔬菜，1996(1)：55-59．4.劉龍洲，蔡潤(rùn)，袁曉君，何歡樂(lè)，潘俊松.黃瓜抗白粉病QTL分子標(biāo)記定位.中國(guó)科學(xué),2008，38(9)：851-8565.劉龍洲，何歡樂(lè)，潘俊松，杜輝，蔡潤(rùn).120份黃瓜種質(zhì)白粉病(Sphaerothecafuliginea)抗性鑒定.種子，2008，27(2)：60-626.劉苗苗.黃瓜霜霉病、白粉病抗病基因的QTL定位[碩士學(xué)位論文].哈爾濱：東北農(nóng)業(yè)大學(xué)，20187.劉苗苗，劉宏宇，顧興芳，張圣平，苗晗.黃瓜白粉病抗性遺傳規(guī)律及分子標(biāo)記研究進(jìn)展.中國(guó)蔬菜，2018，24：7-128.劉秀波，崔琦，崔崇士.瓜類白粉病抗性育種研究進(jìn)展.東北農(nóng)業(yè)大學(xué)學(xué)報(bào)，2005，36(6)：794-7989.呂淑珍，霍振榮，陳正武等.黃瓜霜霉病、白粉病抗性遺傳研究初報(bào).見(jiàn)：李樹(shù)德.中國(guó)主要蔬菜抗病育種進(jìn)展.北京：科學(xué)出版社，1995，436-43810.毛愛(ài)軍，張峰，張海英，張麗蓉，王永健.兩個(gè)黃瓜品種對(duì)白粉病的抗性遺傳分析.中國(guó)農(nóng)學(xué)通報(bào)，2005，21(6)：302-30511.聶京濤，潘俊松，何歡樂(lè)，司龍亭，蔡潤(rùn).中國(guó)蔬菜.2018，10：45-4912.齊建建.黃瓜遺傳多樣性和人工馴化的分子基礎(chǔ)[博士學(xué)位論文].北京：中國(guó)農(nóng)業(yè)科學(xué)院，202013.曲麗，秦智偉.黃瓜白粉病病原菌及抗病性研究進(jìn)展.東北農(nóng)業(yè)大學(xué)學(xué)報(bào)，2007，38〔6〕：835-84114.沈麗平.黃瓜白粉病抗性遺傳分析及相關(guān)QTL初步定位[碩士論文].揚(yáng)州：揚(yáng)州大學(xué)，201815.涂雨辰，田云，盧向陽(yáng)，全基因組關(guān)聯(lián)分析在植物中的應(yīng)用?；瘜W(xué)與生物工程，2020，30〔6〕：7-1016.王明.玉米抗絲黑穗病的全基因組關(guān)聯(lián)分析[博士學(xué)位論文].武漢：華中農(nóng)業(yè)大學(xué)，202017.王建設(shè)，宋曙輝，孟淑春，陳貴林.兩個(gè)甜瓜品種對(duì)白粉病菌的抗性遺傳分析.華北農(nóng)學(xué)報(bào)，2003，18〔2〕：63-6518.汪維鵬，倪坤儀，周國(guó)華.單核苷酸多態(tài)性檢測(cè)方式方法的研究進(jìn)展.遺傳，2006，28〔1〕：117-12619.王振國(guó).黃瓜白粉病抗性基因遺傳規(guī)律和相關(guān)分子標(biāo)記的研究[碩士學(xué)位論文].哈爾濱：東北農(nóng)業(yè)大學(xué)，200720.許啟新,陸世鈞等.1994.黃瓜霜霉病苗期抗性與成株期抗性相關(guān)性的研究.上海農(nóng)業(yè)科技,(1):25~2521.張海英，王振國(guó)，毛愛(ài)軍，張峰，王永健，許勇.與黃瓜白粉病抗病基因嚴(yán)密連鎖的SSR分子標(biāo)記.華北農(nóng)學(xué)報(bào)，2008，23(6)：77-8022.張海英.黃瓜重要抗病基因的分子標(biāo)記研究及遺傳圖譜的構(gòu)建[博士學(xué)位論文].北京：中國(guó)農(nóng)業(yè)科學(xué)學(xué)院，200623.張桂華，杜勝利，王鳴，馬德華.與黃瓜抗白粉病相關(guān)基因連鎖的AFLP標(biāo)記的獲得.園藝學(xué)報(bào)，2004，31(2)：189-19224.張圣平，顧興芳，王燁，苗晗.十一五我們國(guó)家黃瓜遺傳育種研究展.中國(guó)蔬菜，2018，22：1-1025.張圣平，劉苗苗，苗晗，張素勤，楊宇紅，謝丙炎，顧興芳.黃瓜白粉病抗性基因的QTL定位.中國(guó)農(nóng)業(yè)科學(xué)，2018，44(17)：3584-359326.張素勤，顧興芳，張圣平，鄒志榮.黃瓜白粉病抗性遺傳機(jī)制的研究.園藝學(xué)報(bào)，2005，32(5)：899-90127.張素勤，顧興芳，張圣平，鄒志榮.黃瓜對(duì)霜霉病和白粉病抗性的相關(guān)性研究.中國(guó)蔬菜，2007，9：9-1128.張素勤.黃瓜霜霉病和白粉病抗性遺傳機(jī)制及其AFLP分子標(biāo)記研究[學(xué)位論文].楊凌:西北農(nóng)林科技大學(xué)，200529.張艷菊，左紅波，曲麗，秦智偉，周秀艷.黑龍江省黃瓜白粉病病原鑒定.東北農(nóng)業(yè)大學(xué)學(xué)報(bào)，2018，41〔4〕：20-2330.周益林，段霞瑜，盛寶欽.植物白粉病的化學(xué)防治進(jìn)展.農(nóng)藥學(xué)學(xué)報(bào)，2001，3〔2〕：12-1831.AndersonP.A.,LawrenceG.J.,MorrishB.C.,AyliffeM.A.,FinneganE.J.,EllisJ.G.InactivationoftheflaxrustresistancegeneMassociatedwithlossofarepeatedunitwithintheleucine-richrepeatcodingregion.Plantcell1997,9:641-651.32.AranzanaM.J.,KimS.,ZhaoK.,BakkerE.,HortonM.,JakobK.,ListerC.,MolitorJ.,ShindoC.,TangC.,ToomajianC.,TrawB.,ZhengH.,BergelsonJ.,DeanC.,MarjoramP.,NordborgM.Genome-WideAssociationMappinginArabidopsisIdentifiesPreviouslyKnownFloweringTimeandPathogenResistanceGenes.Plosgenetics,2005,1(5):e6033.AriteT,UmeharaM,IshikawaS,HanadaA.,MaekawaM.,YamaguchiS.,KyozukaJ.d14,Astrigolactione-in-sensitivemutantofrice,showsanacceleratedoutgrowthoftillers.PlantCellPhysiol,2018,50(8):1416-142434.AxtellM.J.,StaskawiczB.J.InitiationofRPS2-specifieddiseaseresistanceinArabidopsisiscoupledtotheAvrRpt2-directedeliminationofRIN4.Cell,2003,112:369-377.35.BarnesW.C.,EppsW.M.PowderymildewresistanceinSouthCarolinacucumbers.PlantDiseaseReport1956,40:1093.36.BlockC.C.,ReitsmaK.R.PowderymildewresistanceintheU.S.Nationalplantgermplasmsystemcucumbercollection.HortScience2005,40(2):416-42037.BrueggemanR.,RostoksN.,KudrnaD.,etal.Thebarleystemrust-resistancegeneRpg1isanoveldisease-resistancegenew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