師兄開題報告材料

2014-03-否一、立題依據(jù)（包括研究目的、意義、國內(nèi)外研究現(xiàn)狀和發(fā)展趨勢，需結(jié)合科學(xué)研究發(fā)展趨用前景。附主要參考文獻(xiàn)）H9N2流感是世界康組織WorldHalthOrganizaton，WH）認(rèn)定高病毒一。自166年該首次火雞分鑒定以來野鳥和禽等鳥成為該的主要宿。在亞，該亞的甲型感起的以免疫抑，蛋雞蛋量下降等，并且中東非等其它亞洲國也有爆。近有該亞型甲型已從鳥類到狗、、豬等靈長類物。更要是，在和陸已出了人呼吸道H9N2的臨床例。在中測出H92抗體結(jié)果說該在人群中的可。最重的是，H92亞型提供其內(nèi)的或部分給其他亞型的重組生新的流。稱H9N2供了6內(nèi)部(PA,NS,PB2PB1,NP,)給H7型重組成可人類生命的7N9最近的致死性H1N8流也有H9N2的6個部。同樣有明H9N2感可將其PB1以及結(jié)構(gòu)（NS）供給H51型，從使得H5N1甲型禽流感對有高致性。正可與H7N9和H5N1共在與共一樣，H92可與種亞型的流感存與共。說明H9N2流有爆發(fā)的可，應(yīng)給高度的視。及控制H9N2、H7N9、H5N1、H10N8等人類健康及生命的有極為重要的公共衛(wèi)生學(xué)意HommePJ,EasterdayBC.AvianinfluenzainfectionsICharacteristicsofinfluenzaA-turkey-Wisconsin-1966.AvianDis,1970,14(1):66–74.KawaokaY,ChambersTM,SladenWL,WebsterRG.Isthegenepoolof esinshorebirdsandgullsdifferentfromthatinwildducks?Virology,1988,163:247–AlexanderDJ.Areviewofavianinfluenzaindifferentbirdspecies.VetMicrobio,2000,l74:3–13ArafaAS,HagagNM,YehiaN,ZanatyAM,NaguibMM,NasefSA.EffectofcocirculationofhighlypathogenicavianinfluenzaH5N1subtypewithlowpathogenicH9N2subtypeonthespreadofinfections.AvianDis,2012,56(4DavidsonI,ShkodaI,GolenderN,PerkS,LapinK,KhinichY,PanshinA.GeneticcharacterizationofHAgeneoflowpathogenicH9N2influenza isolatedinIsraelduring2006-2012periods. Genes,2013,46(2):255-263.GuoYJ,KraussS,SenneDA,MoIP,LoKS,XiongXP,NorwoodM,ShortridgeKF,WebsterRG,GuanY.CharacterizationofthepathogenicityofmembersofthenewlyestablishedH9N2influenza lineagesinAsia.Virology,2000,267(2):KimJA,ChoSH,KimHS,SeoSH.H9N2influenzaesisolatedfrompoultryinKoreanlivebirdmarketscontinuouslyevolveandcausethesevereclinicalsignsinlayers.VetMicrobiol,2006,118:169–176.TombariW,PaulM,BettaiebJ,LarbiI,NsiriJ,ElbehiI,GribaaL,A.RiskfactorsandcharacteristicsoflowpathogenicavianinfluenzaisolatedfromcommercialpoultryinTunisia.PLoSOne,2013,ParkerCD,ReidSM,BallA,CoxWJ,EssenSC,HannaA,MahmoodS,SlomkaMJ,IrvineRM,BrownIH.Firstreporteddetectionofalowpathogenicityavian subtypeH9infectionindomesticfowlinEngland.VetRec,2012,ZhangK,ZhangZ,YuZ,LiL,ChengK,WangT,HuangG,YangS,ZhaoY,FengN,FuJ,QinC,GaoY,XiaX.DomesticcatsanddogsaresusceptibletoH9N2avianinfluenza Res,2013,175(1):52-57.SunX,XuX,LiuQ,LiangD,LiC,HeQ,JiangJ,CuiY,LiJ,ZhengL,GuoJ,XiongY,YanJ.Evidenceofavian-likeH9N2influenzaA amongdogsinGuangxi, .InfectGenetEvol,2013,20:471-475.YuH,ZhouYJ,LiGX,MaJH,YanLP,etal.GeneticdiversityofH9N2 esfrompigsin :apotentialthreattohumanhealth?VetMicrobiol,2010,149:254-261.Rui-HuaZ,Hong-YuC,Ming-JuX,KaiL,Hua-LanC,Cun-LianW,DongW,Cun-XinL,TongX.MolecularcharacterizationandpathogenicityofswineinfluenzaH9N2subtype A/swine/HeBei/012/2008/(H9N2).ActaVirol,2011,ZhangK,XuW,ZhangZ,WangT,SangX,ChengK,YuZ,ZhengX,WangH,ZhaoY,HuangG,YangS,QinC,GaoY,XiaX.Experimentalinfectionofnon-humanprimateswithavianinfluenza (H9N2).ArchVirol,2013,158(10):2127-2134.ButtKM,SmithGJ,ChenH,ZhangLJ,LeungYH,XuKM,LimW,WebsterYuenKY,PeirisJS,GuanY.HumaninfectionwithanavianH9N2influenzaAinHongKongin2003.JClinMicrobiol,2005,43(11):5760–5767.ChengVC,ChanJF,WenX,WuWL,QueTL,ChenH,ChanKH,YuenKY.Infectionof promisedpatientsbyavianH9N2in?uenzaA .JInfect,2011,62(5):394–399ComanA,MafteiDN,KruegerWS,HeilGL,FriaryJA,CherechesRM,SirlincanE,BriaP,DragneaC,KaslerI,GrayGC.SerologicalevidenceforavianH9N2influenza infectionsamongRomanianagricultureworkers.JInfectPublicHealth,2013,6(6):438-447HuangR,WangAR,LiuZH,LiangW,LiXX,TangYJ,MiaoZM,ChaiTJ.SeroprevalenceofavianinfluenzaH9N2amongpoultryworkersinSh .EurJClinMicrobiolInfectDis,2013,32(10):1347-LamTT,WangJ,ShenY,ZhouB,DuanL,CheungCL,MaC,LycettSJ,LeungCY,ChenX,LiL,HongW,ChaiY,ZhouL,LiangH,OuZ,LiuY,FarooquiA,KelvinDJ,PoonLL,SmithDK,PybusOG,LeungGM,ShuY,WebsterRG,WebbyRJ,PeirisJS,RambautA,ZhuH,GuanY.ThegenesisandsourceoftheH7N9influenzaescausinghumaninfectionsin .Nature,2013,502(7470):241-244LiuD,ShiW,ShiY,WangD,XiaoH,LiW,BiY,WuY,LiX,YanJ,LiuW,ZhaoG,YangW,WangY,MaJ,ShuY,LeiF,GaoGF.OriginanddiversityofnovelavianinfluenzaAH7N9 escausinghumaninfection:phylogenetic,structural,andcoalescent yses.Lancet,2013,381(9881):1926-1932.ChenH,YuanH,GaoR,ZhangJ,WangD,XiongY,FanG,YangF,LiZhouJ,ZouS,YangL,ChenT,DongL,BoH,ZhaoX,ZhangY,LanY,BaiT,DongJ,LiQ,WangS,ZhangY,LiH,GongT,ShiY,NiX,LiJ,ZhouJ,FanJ,WuJ,ZhouX,HuM,WanJ,YangW,LiD,WuG,FengZ,GaoGF,WangY,JinQ,LiuM,ShuY.ClinicalandepidemiologicalcharacteristicsofafatalcaseofavianinfluenzaAH10N8 infection:adescriptivestudy.Lancet.2014Feb4.pii:MonneI,YamageM,DauphinG,ClaesF,AhmedG,GiasuddinM,SalviatoA,OrmelliS,BonfanteF,SchivoA,CattoliG.ReassortantavianinfluenzaA(H5N1)eswithH9N2-PB1geneinpoultry,Bangladesh.EmergInfectDis.2013,二、研究內(nèi)容和目標(biāo)（說明課題的具體研究內(nèi)容，研究目標(biāo)和效果，以及擬解決的關(guān)鍵科學(xué)問題。此部分為重點闡述內(nèi)容）H9N2流感 毒之一。自1966年該 H9N2的臨床病例。最重要的是，H9N2亞型的 到人類生命的H7N9 、H5N1甲型 等，因此，對于H9N2 迄今為止，H9N2流感 對小鼠的致病機(jī)制尚不十分清楚。我們先前的研究表明了PB2基因的627位點的單個氨基酸突變可以H9N2 機(jī)制。IFITM1-5平均在130個氨基酸，具有共同的保守的CD225區(qū)拓?fù)浣Y(jié)構(gòu)，包括兩段跨膜區(qū)域和一段保守的膜內(nèi)環(huán)狀結(jié)構(gòu)(CIL)。這些IFITM蛋白的共同表達(dá)可能構(gòu)成了機(jī)體抵抗 表明，IFITM蛋白對包括甲型流感 、SARS病 融合。IFITM3被證實具有極高的抗 活性，在小鼠模型中可抑制IAV的 時，機(jī)體的IFITM1和IFITM3的組織學(xué)表達(dá)分布尚不清楚。本研究采用H9N2influenzaA/chicken/Guangdong/V/2008(VK627)毒株，以及8質(zhì)粒反向遺傳系統(tǒng)拯救的，僅在PB2 的627位點單體變的毒株，突變株對小鼠的毒力顯著異于H9N2influenzaA/chicken/Guangdong/V/2008(VK627)毒株。流感 攻毒后，檢測小鼠IFITM1和IFITM3的組織表達(dá)分布，包括心、肝、脾、肺、腎、腦，從而闡釋IFITM1以及IFITM3在流感PB2的627IFITM1、IFITM3H9N2間 SPF雞胚購自 dong/TS/2004(TS)和H9N2influenzaA HI標(biāo)準(zhǔn)陽性 抗體：鼠S1PR1多克隆抗體購自PROTEINTECH( 載體：pMD18-Tvector，購自寶生物工程（大連） ⑵PrimeScriptTM1stStrandcDNASynthesisKit寶生物工程（大連） ) （EB， RNA 2、參照GenBank登錄的小鼠IFIT