個(gè)體化醫(yī)療的現(xiàn)狀與未來課件

個(gè)體化醫(yī)療的現(xiàn)狀與未來四。蛋白質(zhì)微陣列技求個(gè)體化醫(yī)療的現(xiàn)狀與未來個(gè)體化醫(yī)療的現(xiàn)狀與未來四。蛋白質(zhì)微陣列技求ProteomicsTWo-dimensionalelectrophoresislEFstripseparationSDSgelseparationMassspectrometrProteinsequencingPeptidemappingOthersYeasttwohybridassayProteinmicroarray傳統(tǒng)蛋白質(zhì)組研究方法二維凝膠電、質(zhì)譜技求需要復(fù)雜的設(shè)備、樣品的需求量大,可重復(fù)性低,在低豐度蛋白檢測(cè)存在缺陷,且對(duì)蛋白水解為多肽不易控制,不利于可靠的數(shù)據(jù)分析-不能用來作為蛋白質(zhì)生物標(biāo)記物的驗(yàn)AndersonLetal.JPhsiol563(1):23-60,2005個(gè)體化醫(yī)療的現(xiàn)狀與未來個(gè)體化醫(yī)療的現(xiàn)狀與未來個(gè)體化醫(yī)療的現(xiàn)狀1ProteomicsTWo-dimensionalelectrophoresislEFstripseparationSDSgelseparationMassspectrometrProteinsequencingPeptidemappingOthersYeasttwohybridassayProteinmicroarrayProteomics2傳統(tǒng)蛋白質(zhì)組研究方法二維凝膠電、質(zhì)譜技求需要復(fù)雜的設(shè)備、樣品的需求量大,可重復(fù)性低,在低豐度蛋白檢測(cè)存在缺陷,且對(duì)蛋白水解為多肽不易控制,不利于可靠的數(shù)據(jù)分析-不能用來作為蛋白質(zhì)生物標(biāo)記物的驗(yàn)AndersonLetal.JPhsiol563(1):23-60,2005傳統(tǒng)蛋白質(zhì)組研究方法3ProteinMicroarraysProteinMicroarrays4Whatisanarray?areKilometreArraytelescopeCentral-siteArray=impressiveorderedcollectionWhatisanarray?5Theoreticalconsiderationsmacrospot-microspotREkins:ambientanalytetheoryconstantSignaldensitydecreaseSignaldensitlog[signal/areagTotalintensi0.001K0.01K0.1K1K10K100K1000KTotalamountofantibodyTheoreticalconsiderations6AdvantagesofMicroarraysHighsensitivityMuchhigherthroughputMoreflexibilitykLesssampleconsumptiongQuantitationkDirecttargetdetection自0aSmallerisbetterAdvantagesof7個(gè)芯片上可以容納*一種蛋白質(zhì)的所有變異體個(gè)蛋白質(zhì)家族的所有成員條信號(hào)通路中的所有蛋白質(zhì)種組織、器官或有機(jī)體的所有蛋白質(zhì)個(gè)芯片上可以容納8DifferentKindsofProteinArraysAntibodyArrayAntigenArrayLigandArrayDetectionby:SELDIMS,fluorescence,SPR,electrochemical,radioactivity,microcantileverDifferentKindsofProteinArr9抗體微陣列(antibodymicroarray)將抗體作為捕獲分子回定于載體表面,通過抗原抗體反應(yīng),實(shí)現(xiàn)對(duì)樣品中特定蛋白質(zhì)的平行性、高通量檢測(cè)基于既往對(duì)疾病的理解,對(duì)疾病相關(guān)蛋白質(zhì)進(jìn)行高無通量的靶向分析。HaabBBAACr97thAnnualMeetingApril1-5,2006抗體微陣列(antibodymicroarray)10個(gè)體化醫(yī)療的現(xiàn)狀與未來課件11個(gè)體化醫(yī)療的現(xiàn)狀與未來課件12個(gè)體化醫(yī)療的現(xiàn)狀與未來課件13個(gè)體化醫(yī)療的現(xiàn)狀與未來課件14個(gè)體化醫(yī)療的現(xiàn)狀與未來課件15個(gè)體化醫(yī)療的現(xiàn)狀與未來課件16個(gè)體化醫(yī)療的現(xiàn)狀與未來課件17