常用緩沖溶液的配制和PH計(jì)校正溶液配置方法

1、常用緩沖溶液的配制方法1 甘氨酸-鹽酸緩沖液0.05mol/LX毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀釋至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07, 0.2mol/L甘氨酸溶液含15.01克/升2.鄰苯二甲酸-鹽酸緩沖液0.05mol/LX毫升0.2mol/L鄰苯二甲酸氫鉀+0.2mol/LHCl，再加水稀釋到20毫升pH(20 C)XYpH(20C)XY | -2.254.0703.251.4702.453.9603.

2、450.990 /I j2.653.2953.650.5972.852.6423.850.2633.052.022鄰苯二甲酸氫鉀分子量=204.23, 0.2mol/L鄰苯二甲酸氫溶液含40.85克/升3.磷酸氫二鈉-檸檬酸緩沖液pH0.2mol/L Na2HPO4(毫升)0.1mol/L檸檬酸毫升pH0.2mol/L Na2HPO4(毫升)0.1mol/L檸檬酸毫升2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.18 117.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012

3、.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55N&HPO4分子量=14.98, 0.2mol/L 溶液為 28.40 克/升。 Na2HPO4 2H2O

4、 分子量=178.05, 0.2mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量=210.14, 0.1mol/L 溶液為 21.01 克/升。4.檸檬酸-氫氧化鈉-鹽酸緩沖液pH鈉離子濃度檸檬酸克氫氧化鈉鹽酸毫升最終體積mol/LC6H8O7 H2O克NaOH97%HCl 濃升2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610?使用時(shí)可以每升中參加1克克酚，假設(shè)最后pH值有變化，再用少

5、量50%?氫氧化鈉溶液或濃 鹽酸調(diào)節(jié)，冰箱保存。5 檸檬酸-檸檬酸鈉緩沖液 0.1mol/LpH0.1?mol/L 檸檬酸 毫升0.1?mol/L檸檬酸鈉 毫升pH0.1?mol/L檸檬酸 毫升0.1?mol/L檸檬酸鈉 毫升3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.4 ,13.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.

6、210.8檸檬酸C6H8O7 出0:分子量210.14, 0.1mol/L溶液為21.01克/升檸檬酸鈉Na3C6H5O7 2H2O:分子量294.12, 0.1mol/L溶液為29.41克/毫升6.乙酸-乙酸鈉緩沖液0.2mol/LpH18 C0.2?mol/LNaAc毫升0.3?mol/LHAc 毫升pH18C0.2?mol/LNaAc 毫升0.3?mol/LHAc毫升2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.

7、64.905.105.89.400.60Na2Ac 3H2O 分子量=136.09, 0.2mol/L 溶液為 27.22克/升。7.磷酸鹽緩沖液1磷酸氫二鈉-磷酸二氫鈉緩沖液0.2pH0.2?mol/LNa2HPO4 毫升0.2?mol/LNaH2PO4 毫升pH0.2?mol/LNa2HPO4 毫升0.2?mol/LNaH2PO4 毫升5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016

8、.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0N82HP04 2H2O 分子量=178.05, 0.2mol/L 溶液為 85.61 克/升。Na2HPO4 12H2O 分子量=358.22, 0.2mol/L 溶液為 71.64克/升。Na2HPO4 2H2O 分子量=156.03, 0.2mol/L 溶液為 31.21 克/升。2磷酸氫二鈉-磷酸二氫鉀緩沖液1/15mol/LpHmol/L/15Na

9、2HPO4毫升 mol/L/15KH2PO4毫升pHmol/L/15Na2HPO4毫升mol/L/15KH 2PO4毫升4.920.109.907.1.7.003.005.290.509.5078.002.005.911.009.007.39.00一 1.006.242.008.0089.500.506.473.007.007.719.750.256.644.006.0039.900.106.815.005.008.010.0006.986.004.0048.348.678.18Na2HPO4 2H2O 分子量=178.05，1/15M 溶液為 11.876克/升。 KH2PO4分子量=136

10、.09, 1/15M 溶液為 9.078克/升。8.磷酸二氫鉀-氫氧化鈉緩沖液0.05MX毫升0.2MK2PO4+Y毫升0.2NNaOH加水稀釋至29毫升pH20 CX毫升Y 毫升pH20CX毫升Y 毫升5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809 .巴比妥鈉-鹽酸緩沖液18CpH0.04M巴比0.2V鹽酸pH0.04M巴比0.2N鹽酸妥鈉溶液妥鈉溶液毫升毫升6.810018.48.41005.217.010017.88.

11、61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥鈉鹽分子量=206.18;0.04M溶液為8.25克/升10. Tris -鹽酸緩沖液0.05M , 25C50毫升0.1M三羥甲基氨基甲烷Tris溶液與X毫升0.1N鹽酸混勻后，加水稀釋至100毫升pHX毫升pHX毫升7.1045.78.1026.27.2044.78.2022.97.3043.48.30:1997.4042.08.4017.27

12、.5040.38.50:.14.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.908.008.907.0三羥甲基氨基甲烷TrisHOCH2CH2OHCHOCH2NH2 分子量=121.14;0.1M溶液為12.114克/升。Tris溶液可從空氣中吸收二氧化碳，使用時(shí)注意將瓶蓋嚴(yán)11.硼酸-硼砂緩沖液0.2M硼酸根pH0.05M硼砂毫升0.2M硼砂毫升pH0.05M硼砂毫升0.2M硼酸毫升7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B

13、4O7 H2O,分子量=381.43;0.05M溶液=0.2M硼酸根含19.07克/升。 硼酸H2BO3分子量=61.84,0.2M溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。11.硼酸-硼砂緩沖液0.2M硼酸根pH0.05M硼砂毫升0.2M硼酸毫升pH0.05M硼砂毫升0.2M硼酸毫升7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.08.03.07.09.08.0硼砂N&B4O7 10H2O,分子量=381.43;0.05M溶液=0.2M硼酸根含19.07克/升。 硼酸H2BO3,分子量=61.84,0.2M溶液為12.

14、37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。12.甘氨酸-氫氧化鈉緩沖液0.05MX毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀釋至200毫升pHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5才氨酸分子量=75.07;0.2M溶液含15.01克/升。13 .硼砂-氫氧化鈉緩沖液0.05M硼酸根X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀釋至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043

15、.09.65023.010.15046.0硼砂 Na2B4O7 10H2O,分子量=381.43;0.05M 溶液為 19.07克/升。14.碳酸鈉-碳酸氫鈉緩沖液0.1MCa2+、Mg2+存在時(shí)不得使用pH0.1MNa2CO3毫升0.1MN2HCO3毫 升2003709.168.771-99.409.12289.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791Na2CO2 10H2O 分子量=286.2;0.1M 溶液為 28.62 克/升。N2HCO3分子量=84.0;0.1M溶液為8.40克/升。15.“PBS緩沖液PH7.67.47.27.0H2O100010001000100mlNaCl8.58.58.58.5gNa2HPO42.22.22.22.2gNaH2PO40.10.20.30.4gPH計(jì)校正溶液配置的標(biāo)準(zhǔn)方法1 pH4,鄰苯二甲酸氫鉀標(biāo)準(zhǔn)緩沖液：精密稱取在11550枯燥23小時(shí)的鄰苯二甲酸氫鉀KHC8H4O410.12g，加水使溶解并稀釋至 1000ml。2) p