關(guān)于氨基酸和神經(jīng)肽與疼痛關(guān)系的研究

1、 std aa氨基酸分離標(biāo)準(zhǔn)圖氨基酸分離標(biāo)準(zhǔn)圖 csf sample (n = 30)(n = 38) labor pain group(n = 38)pi vs. arginine精氨酸精氨酸 correlation coefficient 相關(guān)系數(shù)相關(guān)系數(shù)p value p值值0.0350.706pi vs. asparagine天冬酰胺酸天冬酰胺酸 correlation coefficientp value-0.207 *0.026pi vs. aspartate天天(門門)冬氨酸鹽冬氨酸鹽 correlation coefficientp value0.285 *0.002pi vs

2、. citrulline瓜氨酸瓜氨酸 correlation coefficientp value0.501 *0.001pi vs. gaba伽馬氨基丁酸伽馬氨基丁酸 correlation coefficientp value0.0190.835pi vs. glutamate谷氨酸谷氨酸 correlation coefficientp value0.0600.518pi vs. glutaminecorrelation coefficientp value-0.601 *0.001pi vs. glycinecorrelation coefficientp value0.332 *0.

3、001pi vs. taurinecorrelation coefficientp value-0.307 *0.001pi pain intensity graded as mild輕度 (n = 11), moderate 中度(n = 18), severe嚴(yán)重 (n = 9) * (p 0.05) * (p 0.01) * (p 0.001) statistically significant correlation relative to the control group.amino acidsage (mean sd)control groupa (mol/l)(n = 30)3

4、3.4 5.0labor pain groupb(mol/l)(n = 38)28.6 4.5alanine丙胺酸丙胺酸 29.41 1.3333.41 1.53cystine胱氨酸胱氨酸 0.6 0.12 0.2 0.06*histidine組氨酸組氨酸 14.87 1.1317.39 0.84isoleucine異亮氨酸異亮氨酸 3.77 0.154.03 0.19leucine亮氨酸亮氨酸 8.56 0.379.09 0.35lysine賴氨酸賴氨酸 18.72 1.3819.34 1.31methionine蛋氨酸蛋氨酸 1.85 0.132.19 0.10*phenylalanine

5、苯基丙氨酸苯基丙氨酸 7.26 0.297.94 0.27proline脯氨酸脯氨酸 0.89 0.091.22 0.09*serine絲氨酸絲氨酸 32.05 1.2724.55 0.87*threonine蘇氨酸58.68 2.7359.94 3.16tryptophan色氨酸1.87 0.102.26 0.10*tyrosine酪氨酸3.94 0.434.95 0.44valine纈氨酸9.59 0.5010.02 0.38 腦脊液中生理氨基酸和疼痛的聯(lián)系 amino acids氨基酸氨基酸age (mean sd)年齡（平均數(shù)年齡（平均數(shù) 方差）方差）controla (mol/l)(

6、n = 35)37.6 12.2標(biāo)準(zhǔn)組chronic painb (mol/l)(n = 29)64.7 12慢性疼痛組acute painc (mol/l)(n = 46)36.8 19.1急性疼痛組*results chronic pain vs no pain group aspartate, glutamate, glycine and no involved in chronic pain perception 天門冬氨酸鹽，谷氨酸，甘氨酸和一氧化氮與疼痛的感知有關(guān) chronic pain vs acute pain differential roles of amino acid

7、s in chronic and acute pain states氨基酸在慢性和急性疼痛狀態(tài)的不同作用 aspartate - a key player in pathogenesis of chronic pain 天門冬氨酸鹽導(dǎo)致慢性疼痛的重要因素 identificationcharacterisationquantificationlocalisationcns effectsantagonistspain models mkilfcdllllslfssvssscqkdclvcreklrptldsfslemcileceekaftsplwtpbovine60mkvllcdllllslf

8、ssvfsscqrdcltcqeklhpaldsfdlevcileceekvfpsplwtphuman60mkilfcdvlllsllssvfsscprdcltcqeklhpapdsfnlktcilqceekvfprplwtvmouse60mkilfcdvlllsllssvfsscpedcltcqerlhpapgsfnlklcilqceekvfprplwtlrat60signal peptidectkvmargswqlspadpdhvaaaldqprasemqhibovine108ctkvmarsswqlspaapehvaaalyqprasemqhihuman108ctkvmasgsgqlsp

9、adpelvsaalyqpkasemqhimouse119ctkamasdseqlspadpeltsaalyqskasemqhirat113krmprvrslfqrq-bovine121rrmprvrslfqeq-human121krmprvrslvqvrdaepgadaepgmouse132krmprvrsvvqardaepea-rat127bpnp-2krtepgleevgeieqkqlqkrfggftgarksarklanqbovine159eepepgmeeagemeqkqlqkrfggftgarksarklanqhuman159adaepgaddaeeveqkqlqkrfggftga

10、rksarklanqmouse170-daepvadeadeveqkqlqkrfggftgarksarklanqrat164bpnp-3 (nocistatin)nociceptinkrfsefmrqylvlsmqssqrrrbovinekrfsefmrqylvlsmqssqrrrhumankrfsefmrqylvlsmqssqrrrmousekrfsefmrqylvlsmqssqrrrratbpnp-4tlhqngna176tlhqngnv176tlhqngnv187tlhqngnv181bpnp-5immunofluorescent confocal images of sections

11、of mouse brain incubated with anti-nocistatin antiserum. a transverse section of mouse brain was incubated with anti-mpnp-3 antibody followed by cy3-conjugated anti-rabbit igg. bar, 250 m (a, b, c, d); 100 m (c, d). human nocistatin and nociceptinrrmprvrslfqeq-eepepgmeeagemeqkqlqkrfggftgarksarklanqkrhuman148krmprvrslvqvrdaepgadaepgadaepgaddaeeveqkqlqkrfggftgarksarklanqkrmouse159krmprvrsvvqardaepea-daepvadeadeveqkqlqkrfggftgarksarklanqkrrat153krmprvrslfqrq-krtepgleevgeieqkqlqkrfggftgarksarklanqkrbovine148nociceptin96969696nocistatinnocistatin okuda-ashitaka e, tachibana s et al, noci