人肌紅蛋白基因外顯子一的生物信息學(xué)研究

1、1 / 18人肌紅蛋白基因外顯子一的生物信息學(xué)分析一.Introduction: Myoglob in, the article is composed of a peptidecha ins and a binding prote in of heme prosthetic group, is withinthe muscle oxygen storage protein, its oxygen saturation of theelliptic curve.Myoglobin is the main protein of skeletal muscle andcardiac muscle,

2、 whe n muscle damage, leakage to the circulating inthe blood in muscle tissue, increase the serum concen tratio n ofmyoglob in, the in dex is used to determ ine whether muscledamage.二.Purpose:對(duì)人肌紅蛋白基因外顯子一基因序列使用“ Bioed 和 DNAMAN、 anthe5.0 及相關(guān)網(wǎng)站進(jìn)行 GC 含量統(tǒng)計(jì)、 電 子酶切、引物設(shè)計(jì)、基因在人類(lèi)基因組中的定位、電子克隆及蛋 白在細(xì)胞內(nèi)的定位、氨基酸組成統(tǒng)

3、計(jì)、分子量、等電點(diǎn)、親疏水 性、信號(hào)肽分析 跨膜區(qū)域）、二級(jí)結(jié)構(gòu) 含保守結(jié)構(gòu)域）及三維 結(jié)構(gòu)分析，miR 分析，啟動(dòng)子分析等。其中抗原表位分析、多序 列比對(duì)和進(jìn)化分析等。三.The n ature and fun cti on of myoglob in :Nature: myoglob in = a polypeptide cha in + a prosthetic grouppolypeptide cha in, composed of 153 amino acid residues Prostheticgroup: heme prosthetic groupMolecular weigh

4、t: 16, 700Fun cti on: tran sport of oxyge n and oxyge n storage fun cti on in2 / 18muscletertiary structure of myoglob in四.Key word:肌紅蛋白 myoglobin)五.Implements and methods采用Bioedit和DNAMA分析軟件工具和在線有關(guān)蛋白質(zhì)和核酸方面的分 析軟件，分別進(jìn)行核酸和蛋白質(zhì)兩個(gè)方面的分析。核酸分析首先從 pubmec 核酸數(shù)據(jù)庫(kù)中搜索到人的Myoglobin 核酸序列，分別進(jìn)行序列的基本性質(zhì)分析，限制性酶譜分析及其基因 在染色

5、體上的定位分析。具體操作方法將根據(jù)上述各個(gè)軟件上“ Help 文件進(jìn)行，基因在染色體上的定位分析根據(jù)網(wǎng)頁(yè)上的操作說(shuō)明進(jìn)行。從 pubmed 蛋白質(zhì)數(shù)據(jù)庫(kù)中搜索到人的 Myoglobin 和整個(gè)前體 Myoglobin 蛋白質(zhì)序列，分別進(jìn)行蛋白序列的基本性質(zhì)分 析 ORGANISM Homo sapie nsEukaryota。Metazoa。Chordata。Craniata。VertebrataEuteleostomi。Mammalia。Eutheria。Euarchontoglires。Primates。Haplorrhi ni。Catarrhini。Hominidae。Homo.REFE

6、RENCE 1 (bases 1 to 2552AUTHORS Akaboshi,E.TITLE Cloning of the huma n myoglob in geneJOURNAL Ge ne 33 (3, 241-249 (1985PUBMED 2989088COMMENT Origi nal source text: Huma n: fetal liver DNA (library ofLaw n etal., clone lambda-HMB151。delta-thalassemia leukocyteDNA (libraryof Kimura et al., clone lamb

7、da-HMB159.Draft entry and computer-readable copy of seque nee 1ki ndlyprovided by E.Akaboshi, 22-OCT-1985.A tan dem repeat seque nee is found at positi on 142-794.Positi on ofthe mRNA cap site was deduced from that of the sealmyoglob in gene(Bla nchetot et al. 1983.FEATURESLocatio n/Qualifierssource

8、 1.2552/orga ni sm=Homo sapie ns/mol_type=genomic DNA/db_xref=taxo n: 9606/map=22q11.2-qterrepeat_region 1100.1750/no te=Fok family repetitive eleme ntprim_tra nscript 1895.2552/no te=myoglob in mRNA and in tr onge nejoi n(1965.2552,M14602.1:1.2671,M14603.1:1.501/gen e=MBCDSjoi n(1965.2059,M14602.1:

9、1415.1637,M14603.1:355.501/gen e=MB/no te=myoglobi n/codo n_ start=1/protein_id=AAA59595.14 / 18/db_xref=GI:386872/tra nslati on=MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAEGAMNKALELFRKDMASNYKELGFQGexon2552/gen e=MB

10、/no te=myoglob in intron AORIGIN 2 bp upstream of RsaI site.1 gtactgtatt ttcattcctc ttagttatct ccctaaaaag actctgagttccttgaacac61 aggaaggtgt tttatttgat tttgttatcc tcagcatgta gcagtgtctgacacacagta121 ggtgctctat cactgtgaga gggatggatg gatgggtgga gttacagatg gatagaagga181 tagatggagg gatgggtgga tgatggatgg a

11、tagatggat ggagggggga tgatgaatgg241 agggataatg agtggatgaa tgagggaatg ggtggatgga tggatggagg gatggaggaa301 cagatagata gatggaggga tgggtgggtg atggatggat agatggatggagggagggat361 gatgaatgga gggataatga atggatgaat gaggggatgg gtggatggat gaatggaggg421 atgatgggtg gatgaatgaa ttgagggatg gatggatgaa cacatggatg gatg

12、gataga481 tggatagatg gaggaactgg tggattttgg atggatgggt ggatggatag atgaatgaat541 gcctggatag acaaagagat gatggataga tgaatagatg aattaagggatgtcggatag601 atggagggat tgatagatgt tggatggatg ggtggtggat ggatagatgagtgaatgcat661 ggatagacaa agagatgatg gatggatgaa ttaagggatg acagatggatggatggatga721 gtaactggat ggacaa

13、gtgg ataaatggat agatggttga atacctgaat ggattgaagg781 aggatgcatg gatgtaagat aaggctaatc atcctccact ctctttcttt gcaaaaccat841 ccacccattt actcaataaa catttattca gttcaaactt ggcacaaagc accatgtgag901 gcccaagaga tacgtgggtt aataaaacag agctcctgcc ctcctgaaaactgcaaagaa961 aggggcgtgg cttcctgagt tcaaatccca actctgcca

14、g cgactagctg tacatcagtg1021 atgtttccct actttctctc aattaaatag ggataatgtc agtacctatc acattgggag1081 gtcttgcggg gattaaatga gttaccaaat gccaagtgtt tgggacaggg cctggcaccc1141 agcaaagtct cttgtgagtg ctggctgcta ttatcctaat ggagaagatg gcatgaaaac1201 caggaaatag gatgcccttt gggaagcaat gcaacaggaa cttacacaaa gaaagga

15、aag1261 gaggaagcaa ttagtggtgt ctcaaaggag tatgtcaaga aaaacttttc agagggaaac1321 ctttgagcag ggtcatgaaa acaggagttc tctaagagat tgtggacttg cctgggacca5 / 181381 cctggctata agcacaaaac catccggttc ctttctgtca cttctggcgg gtgaggggtc1441 tctggcaaag gggcagaagg tgcgtgagag gttgcgaatg gccaggactg tcctggggcc1501 agccgg

16、ggca cctggtggcc aagcttagaa acatgacagg tcctcttggg agggctgacc1561 gcagggagcg ttgggtttca ggctgctggc gtcggcttct gtggtgccct ttctgtcggc1621 tatgagagtc cagacagtgc ccaacctcct ccccttcttt ccacacgcac aaccacccca1681 ccccctgtgg cctgagctgt cctgcctcgc cacaatggca cctgccctaa aatagcttcc1741 catgtgaggg ctagagaaag gaaa

17、agatta gaccctccct ggatgagaga gagaaagtga1801 aggagggcag gggaggggga cagcgagcca ttgagcgatc tttgtcaagc atcccagaag1861 gtataaaaac gcccttggga ccaggcagcc tcaaacccca gctgttgggg ccaggacacc1921 cagtgagccc atacttgctc tttttgtctt cttcagactg cgccatgggg ctcagcgacg1981 gggaatggca gttggtgctg aacgtctggg ggaaggtgga gg

18、ctgacatc ccaggccatg2041 ggcaggaagt cctcatcagg taaaaggaag agattccatt gcccctgcca cccacaccct2101 aagatcaagg gtgttcagct gcaaggtgga aagtttgcac gtggggtagg tcagttggct2161 gcattagtta agggtgttag aacggtcact tgctttttct ttgcttttaa gtgtcaggga2221 ttggactcag gagagggaaa ggagccattt caggctgatg tcagcagctg gaggaagcat2

19、281 gagaatcaaa cctaggatgc tcagagtcca ccaggaagaa ttttagaattatagacagtc2341 agagttaaca agggtcctga gagattttgt acagccacct ctcttacaggatgaggacaa2401 aaagcgactg agaaggggag gacatttcca gagtcacagc tcattaaatgctcttaaagt2461 gtcaaggtta agacatgctc ttcaagggga gacagatctg gttctagacttggctctgcc2521 actgagccac tgggtgacc

20、t ttgggaaggt acOfficial SymbolMBprovided by HGNCOfficial Full Namemyoglob in provided by HGNCPrimary source :HGNC:6915Locus tag :RP4-569D19.7Seerelated:Ensembl:ENSG00000198125。HPRD:01170。MIM:160000。Vega:OTTHUMG00000150606Gene type:prote in cod ingRefsep status: REVIEWEDOrganism : Homo sapiensLin eage : Eukaryota。Metazoa。Chordata。Crani ata。Vertebrata。Euteleostomi。Mammalia。 Eutheria。 Euarchontoglires。 Primate