白術(shù)內(nèi)酯1抗炎

1、Li He2016.10.18 Atractylenolide I protects mice from LPS-induced acute lung injuryEuropean Journal of Pharmacology 765 (2015) 94-99Introductionlung inflammationedema neutrophil accumulationAcute respiratory distress syndromeALI(acute lung injury)sepsisburnssevere pulmonary infections Atractylenolide

2、 I (AO-I)Introductionanti-inflammatory effect LPS IL-6TNF-noet alRhizoma Atractylodes macrocephalaRAW 264.7IntroductionLPSTLR41. Reagents purchase 2. Animals Male BALB/c mice (2024 g)3. Mouse model of LPS-induced ALI Control LPS LPS + AO-I (5、10 and 20 mg/kg) LPS + VGX (0.5 mg/mouse) Materials and m

3、ethods72 mice5. Wet-to-dry lung weight ratio (W/D ratio) collecte lungs blotted dry wet weight 80 C for 48 h dry weight.4. Histopathologic studies of LUNG 10% formalin sliced into 5 m sections light microscopeMaterials and methods8. ELISA assay ELISA kit 9. Western blot analysis10. Statistical analy

4、sis6. MPO activity assay MPO kit7. Cells counts BALF 3000g for 7 min PBS cell count Materials and methodsFig. 1. Effects of AO-I on histopathological changes in lung tissues in LPS-induced ALI mice. Mice were given an intraperitoneal injection of AO-I (5, 10 and 20 mg/kg) 1 h after administration of

5、 LPS. Lungs tissues from each experimental group were processed for histological evaluation at 6 h after LPS challenge. Representative histological changes of lung obtained from mice of different groups. A: Control group, B: LPS group, C: LPS+AO-I (5 mg/kg) group, D: LPS+AO-I (10 mg/kg) group, E: LP

6、S+AO-I (20 mg/kg) group, F: LPS+VGX-1027 (0.5 mg/mouse) (Hematoxylin and eosin staining, magnification 200 X ).ResultsFig. 2. Effects of AO-I on MPO activity in lung tissues of LPS-induced ALI. MPO activity was determined at 6 h after LPS administration. The values presented are the mean S.D. (n=12

7、in each analysis) of three independent Experiments. P#0.01 vs. control group, P*0.05, P*o0.01 vs. LPS group.Fig. 3. Effects of AO-I on the lung W/D ratio of LPS-induced ALI mice. The lung wet/dry weight ratio was determined at 6 h after LPS challenge. The values presented are the means S.D. (n=12 in

8、 each analysis) of three independent experiments. P#0.01 vs. control group, P*0.05, P*0.01 vs. LPS group.ResultsFig. 4. Effects of AO-I on inflammatory inflammatory cells infiltration in the BALF. The values presented are mean S.D. (n=12 in each analysis) of three independent experiments. P#0.01 vs.

9、 control group, P*0.05, P*0.01 vs. LPS group. ResultsFig. 5. Effects of AO-I on TNF-, IL-6, IL-1, IL-10, IL-13, and MIF production in the BALF of LPS-induced ALI mice. BALF was collected at 6 h following LPS challenge to analyze the inflammatory cytokines TNF-, IL-6, IL-1, IL-10, IL-13, and MIF. The

10、 values presented are mean S.D. (n=12 in each analysis) of three independent experiments. P#0.01 vs. control group, P*0.05, P*0.01 vs. LPS group. ResultsResultsFig. 6. AO-I inhibits LPS-induced TLR4 expression, NF-B activation and IB degradation. The values presented are mean S.D. (n=12 in each analysis) of three independent experiments. P#0.01 vs. control group, P*0.05, P*0.01 vs. LPS group. Conclusion This study reported for the first time that AO-I inhibited LPS-induced ALI in mice. The anti-in