(四)、中藥毒理實(shí)驗(yàn)室

1、（四）、中藥毒理實(shí)驗(yàn)室實(shí)驗(yàn)室名稱中藥毒理實(shí)驗(yàn)室管理人員江素瑛 助理教授電話分機(jī)1614實(shí)驗(yàn)室簡介進(jìn)行有關(guān)“有毒中藥與其有毒成分“的體內(nèi)外基礎(chǔ)研究、“常用妊娠中藥與保健食品“之毒理安全性評估、建立“含馬兜鈴酸中草藥“之早期細(xì)胞損傷指標(biāo)及其應(yīng)用於腎病患者之分子流行病學(xué)研究：另一方面也研究“有毒中藥”的配伍應(yīng)用機(jī)理、中草藥抑制血管新生作用、與中草藥之化學(xué)防護(hù)與輻射保護(hù)作用，用科學(xué)數(shù)據(jù)和現(xiàn)代理論來提供科學(xué)依據(jù)，以闡述中藥的安全性和有效性。研究重點(diǎn)1. 馬兜鈴酸與含馬兜鈴酸中草藥與臨床腎病變之相關(guān)研究2. 馬兜鈴酸與含馬兜鈴酸中草藥對人類腎細(xì)胞之毒理基因圖譜研究3. 中草藥配伍對馬兜鈴酸與含馬兜鈴酸中草

2、藥毒性作用之影響(體內(nèi)與體外研究)4. 中草藥對乳癌輻射增敏劑與保護(hù)劑的開發(fā)5. 中草藥之化學(xué)防護(hù)與輻射保護(hù)作用研究(體內(nèi)與體外研究)6. 中草藥對抑制血管新生之研究7. 中草藥與保健食品之毒理安全性評估(體內(nèi)與體外研究)8. 農(nóng)藥殘留之健康風(fēng)險(xiǎn)評估9. 農(nóng)藥與工業(yè)化學(xué)物之遺傳毒性研究(體內(nèi)與體外研究)儀器設(shè)備螢光照相系統(tǒng)(Fluorescence microscope), Laminar flow, CO2 incubator, PCR ( Hybaid ), Gel eletrophoresis systems（Vertical and horizontal）, DNA analysis

3、apparatus, RNA analysis apparatus, protein analysis apparatus, (operating microscopes), Table-top ultracentrifuge,分析天平, pH meter, 超音波洗淨(jìng)機(jī)重要研究成果1. Chao-Min Chen, Su-Yin Chiang, and Ning-Hsing Yeh. Increased stability of nucleolin in proliferating cells by inhibition of its self-cleaving activity. Jour

4、nal of Biological Chemistry, 1991, 266: 7754-7758. (SCI)2. 2.R. Rita Misra, Su-Yin Chiang, and James A. Swenberg. (1994) A Comparison of Two Ultrasensitive Methods for Measuring 1,N6-Etheno 2 Deoxyadenosine and 3,N4-Etheno 2 Deoxycytidine in Cellular DNA. Carcinogenesis, 15, 1647-1652. (SCI)3. Su-Yi

5、n Chiang, James A. Swenberg, Wade H. Weisman, and Thomas R. Skopek. (1997) Mutagenicity of Vinyl Chloride and Its Metabolites Chloroethylene oxide and Chloroacetaldehyde in Metabolically-competent Human Lymphoblastoid Line. Carcinogenesis, 18, 32-35. (SCI)4. Nakamura, J., Walker, V.E., Upton, P.B.,

6、Chiang, S-Y, Kow, Y.W., and Swenberg, J.A. (1998) Highly sensitive AP site assay can detect spontaneous and chemically-induced depurination under physiological conditions. Cancer Research, 58(2):222-225. (SCI)5. Su-Yin Chiang, James A. Swenberg, Wade H. Weisman and Thomas R. Skopek. Mutagenicity of

7、vinyl chloride and its reactive metabolites chloroethylene oxide and chloroacetaldehyde, in a metabolically competent human B-lymphoblastoid line. Carcinogenesis, 1997, 18: 31-36. (SCI)6. Nakamura, J., Walker, V.E., Upton, P.B., Chiang, S-Y, Kow, Y.W., and Swenberg, J.A. Highly sensitive Apurinic/Ap

8、yrimidinic site assay can detect spontaneous and chemically-induced depurination under physiological conditions. Cancer Research, 1998, 58: 222-225. (SCI)7. Natalia Yu. Tretyakova, Su-yin Chiang, Vernon E. Walker, and James A. Swenberg. Quantitative analysis of 1,3-butadiene-induced DNA adducts in v

9、ivo and in vitro using Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry. Journal of Mass Spectrometry, 1998, 33:363-376. (SCI)8. Ching-Liang Hsieh, Nou-Ying Tang, Su-Yin Chiang, Ching-Tou Hsieh and Jaung-Geng Lin, Anticonvulsive and free radical scavenging actions of two herbs,

10、 Uncaria Rhynchophylla (Miq) Jack and Gastrodia elata Bl., in kainic acid-treated rats. Life Sciences, 1999, 65(20): 2071-2082. SCI9. Hsieh C.L., Chiang S.Y., Tang N.Y., Hsieh C.T. Time course of beavior and generation of free radicals in kainic acid-induced epileptic seizure. Acta Neurologica Taiwa

11、nica, 1999, 8(1): 63-64. 10. Ching-Liang Hsieh, Chin-Hsien Chang, Su-Yin Chiang, Tsai-Chung Li, Nou-Ying Tang, Chu-Zong Pon, Ching-Tou Hsieh, Jaung-Gene Lin, Anticonvulsive and free radical scavenging activities of vanillyl alcohol in ferric chloride-induced epileptic seizures in Sprague-Dawley rats

12、. Life Sciences, 2000, 67(10): 1185-1195. (SCI)11. 謝慶良、李佳容、陳春榮、江素瑛、唐娜櫻、謝慶竇、林昭庚，Vanillyl alcohol對Kainic acid誘發(fā)大白鼠癲癇發(fā)作的抗癲癇作用和機(jī)轉(zhuǎn).Taiwan Journal of Chinese Medicine. 2001；1(1) 19-3412. Hsieh Ching-Liang, Liao En-Tzu, Chiang Su-Yin, Lao Chih-Jui, Tang Nou-Ying, Hsieh Ching-Tou, Lin Jaung-Geng: Effect of

13、Rhizoma Corydalis on focal cerebral infarct in ischemia-reperfusion injured rats Acta Pharmacol Sin. 2001; 22(12): 1143-1148. (SCI)13. Ching-Liang Hsieh, Su-Yin Chiang, Ken-Sheng Cheng, Nou-Ying Tang, Chia-Jung Lee, Chu-Zong Pon, Ching-Tou Hsieh, Jaung-Geng Lin. Anticonvulsive and free radical scave

14、nging activities of Gastrodia elata BL. In kainic acid-treated rats. Amer. J. Chin. Med. 2001, 29(2):331-341. (SCI)14. 14.C.L. Hsieh, E.T. Liao, S.Y. Chiang, C.J. Lao, J.G. Lin, J.S. Kuo. Effect of Rhizoma Corydalis on cerebral infarct rats. J. Cerebral Flow and Metabolism. 2001, 21(suppl. 1): S490,

15、 (SCI)15. 謝慶良，林慧怡，李佳容，唐娜櫻，江素瑛，謝慶竇，林昭庚，鉤藤五種萃取物對kainic acid誘發(fā)大鼠腦組織脂質(zhì)過氧化作用效用之比較Taiwan J. Chin. Med. 2002; 1(3): 11-24.16. Chih-Jui Lao, Ching-Liang Hsieh, Jon-Son Kuo, Su-Yin Chiang, Shu-Chao Chen, En-Tzu Liao, Juang-Geng Lin. Effect of Salvia miltiorrhiza Bunge on cerebral infarct in ischemia-reperfus

16、ion injured rats. Amer. J. Chin. Med. 2003, 31(2):191-200 (SCI)17. 謝慶良、江素瑛、唐娜櫻、謝慶竇、許昇峰、彭主榮、林昭庚，Catechin對Kainic acid誘發(fā)癲癇發(fā)作大鼠的自由基清除作用。Taiwan Journal of Chinese Medicine，2(1)：41-54，2003。18. 吳琇卿、江素瑛、陳忠川、黃蕙棻，豬苓多糖對化療藥物誘發(fā)之遺傳損傷的防護(hù)作用，Taiwan Journal of Chinese Medicine，2(2) ：70-79，200319. 謝慶良、劉德蕙、林昭庚、許昇峰、姥芝瑞、

17、江素瑛、謝慶竇、唐娜櫻，補(bǔ)陽還五湯改善缺血再灌流腦梗塞大鼠與一氧化氮的關(guān)係，臺灣中醫(yī)醫(yī)學(xué)雜誌，2004，3(2)，20 35。20. 唐娜櫻、錢添發(fā)、李采娟、江素瑛、李珮端、林昭庚、謝慶良*，槐花對ibotenic acid誘發(fā)大白鼠學(xué)習(xí)記憶障礙效用之研究，J Chin Med，2004，15(2)，99 108。21. Kuen-Yuh Wu, Su-Yin Chiang*, Tai-Hung Huang, Yu-Shan Tseng, Yi-Lung Chen, Hsien-Wen Kuo, Ching-Liang Hsieh. Influence of Lifestyle Factors

18、 upon the Formation of N-(2-Hydroxyethyl)Valine for Smokers and for Nonsmokers, Mutation Research, 559(1-2):73-82, 2004 (SCI)(*: corresponding author)22. Shung-Te Kao .Cheng-Hung Chang .Yueh-Sheng Chen . Su-Yin Chiang . Jaung-Geng Lin. Effects of Ding-Chuan-Tang on bronchoconstriction and airway leu

19、cocyte infiltration in sensitized guinea pigs, Immunopharmaology and Immunotoxicology, 26(1):113-24，2004 (SCI)23. Su-Yin Chiang, Tai-Hung Huang, Shi-Nian Uang, Hong-Dar Isaac Wu, Yi-Chiech Wei, Hui-Yi Lin, James A. Swenberg and Kuen-Yuh Wu. Analysis of 7-methylguanine using isotope dilution and gas chromatography/ electron-capture negative chemical ionization mass spectrometry, Rapid Communications In Mass Spectrometry. 2005; 19: 1624. Ching-Liang Hsieh, Chi-Long Chen, Nou-Ying Tang, Chin-Min Chuang, Ching-Tou Hsieh, Su-Yin Chiang, Jaung-Geng Lin and Sheng