常用引物序列（分子生物學(xué)）

1、.#:第4頁常用引物序列分子生物學(xué)序列5-3 GGTTACCTTGTTACGACTT AGAGTTTGATCCTGGCTCA GACGCACAATCCCACTATCC AGATGGTGCACGATGCACAG GGCAAATGGCATTCTGACAT TAAGAGTCACTTTAAAATTTGTATAC TACCACTACAATGGATGATG GACTGGTTCCAATTGACAAGC TCATCGGAAGAGAGTAG CCCTCATAGTTAGCGTAACG TACTATTGCCAGCATTGCTGC AACCATCTCGCAAATAAATA ACGCACAGAATCTAGCGCTT T

2、AGAAGGCACAGTCGAGG TTAGGACAAGGCTGGTGG ATAACCCCGCCCCGTTG CGCAAATGGGCGGTAGGCGTGATGGGCGGTAGGCGT G TCGTTGGGCGGTCAGC GATTATGCGGCCGTGTACAA GATCTCGACGCTCTCCCT TTGTACACGGCCGCATAATC GTGGTTTGTCCAAACTCATC AGCACCCAGTCCGCCCTGAGC CGTCGCCGTCCAGCTC AACATTTTCGGTTTGTATTACTTC TGTATCTTATGGTACTGTAACTG CTTTATGTTTTTGGCGTCT

3、TCCAGLP4 H1-FH1 HSVtk-pArev IRES-R ITS4 ITS5 M13-47 M13FTrxReverseM13-20M13F-4 0 M13R malE MT-Profor mU6F2mouse NL1 NL4 NOSR-primer P1 p10-Profor P2 pBABE3 PBABE5 pBADfw pBADrvpTrcHis-rv PBV220F pCDNA3.1F pCMV5F pCMV5R PCMV-F PCMV-RGCCCTTCTTAATGTTTTTG TCGCTATGTGTTCTGGGAAA GTCTCCTTCCGTGTTTCAG CCTCACA

4、TTGCCAAAAGACG TCCTCCGCTTATTGATATGC GGAAGTAAAAGTCGTAACAAGG CGCCAGGGTTTTCCCAGTCACGAC TGTAAAACGACGGCCAGT CAGGAAACAGCTATGACC GGTCGTCAGACTGTCGATGAAGCC CATCTCAGTGCAACTAAA TAGTATCCGACGCCGCCATC GCATATCAATAAGCGGAGGAAAAG GGTCCGTGTTTCAAGACGG CGTCCAGCTCCATGTTGC CCAGGCTTTACACTTTATGC CGGACCTTTAATTCAACCC GCGATTAAG

5、TTGGGTAACGC ACCCTAACTGACACACATTCC CTTTATCCAGCCCTCAC CCATAGCATTTTTATCCATAAG ATCTGTATCAGGCTGAAAATC AAGAAGGGCAGCATTCAAAG CTAGAGAACCCACTGCTTAC TTCCAAAATGTCGTAATAAC ATTATAGAGGACACCTAGTC TCTAAAAGCTGCGGAATTGT TCCAAACTCATCAATGTATCpcoldI-RpCold-SUMO-R pCold-TF-F1 pDBLeu-F pDEST22-F pDEST22-R PDONR-F PDONR-R

6、pDsRED-ex-C1-F PEF-FEF-1aForward pEGFP-C-3 pEGFP-C-5 pEGFP-N-3EGFP-Nrev pEGFP-N-5 pFastBac-fw pFastBac-R pFastBac-rev pFlag-CMV-F pFlag-CMV-R PGEX-F PGEX-R PH-Profor pIRESrv PJET1.2-F PJET1.2-R pLNCX-F pLXSN-F pLXSN-R pMAL-C2X-F pMAL-C2X-RGGCAGGGATCTTAGATTCTG CCACTTTCAACGAGCTGATG GAATAAGTGCGACATCATC

7、ATC TCGATGATGAAGATACCCCACC CTCGACGTCTTACTTACTTAGC TCGCGTTAACGCTAGCATGGATCTC GTAACATCAGAGATTTTGAGACAC TCCCACAACGAGGACTACAC TCAAGCCTCAGACAGTGGTTC TATGGCTGATTATGATCAGT CATGGTCCTGCTGGAGTTCGTG CGTCGCCGTCCAGCTCGACCAG TGGGAGGTCTATATAAGCAGAG GTCCGAAACCATGTCGTAC TGGACAAACCACAACTAGAATG CCTCTACAAATGTGGTATGG GG

8、TAGGCGTGTACGGTGG GCACTGGAGTGGCAACTT GGCAAGCCACGTTTGGTG GAGCTGCATGTGTCAGAGG AAATGATAACCATCTCGC GCCCTAGATGCATGCTCG CGACTCACTATAGGGAGAGCGGC AAGAACATCGATTTTCCATGGCAG AGCTCGTTTAGTGAACCGTCAGATCG CTTGAACCTCCTCGTTCGAC GTTGCTGACTAATTGAGATG TGCGTACTGCGGTGATCAAC CTGCAAGGCGATTAAGTTGGpMAL-C5x-R PPC86-FpDEST22-F2

9、019 PPC86-RpDBLeu-R pQE30-F pQE30-R pSHUTTLE-CMV-R pSilencer4.1-F2.0rev PTRC99C-F PTRC99C-RPBV220R pYes2.RCYC1-Terminator RFP-Nrev RV-M RVP3 RVP4 S.tag SeqL-A SeqL-B SP6 SV40 SV40-pArevpSG5rv T3 T7 T717base T7ter U6-promoterhuman v1.5 V5rev WPRE-R XL39CMV-24TGTCCTACTCAGGAGAGCGTTCAC TATAACGCGTTTGGAAT

10、CACT GTAAATTTCTGGCAAGGTAGAC TGAGCGGATAACAATTTCAC GTTCTGAGGTCATTACTGG GTGGTATGGCTGATTATGATCAG AGGCGATTAAGTTGGGTA TTGCGCCGACATCATAAC CTGCGTTCTGATTTAATCTG GCGTGAATGTAAGCGTGAC GTTCACGGTGCCCTCC AGCGGATAACAATTTCACACAGGA CTAGCAAAATAGGCTGTCCC GACGATAGTCATGCCCCGCG GAACGCCAGCACATGGAC GCAGTTCCCTACTCTCGC CATCAGAGATTTTGAGACAC ATTTAGGTGACACTATAG GGAGGCTTTTTTGGAGGC GAAATTTGTGATGCTATTGC ATTAACCCTCACTAAAGGGA TAATACGACTCACTATAGG ACA