重組人腫瘤壞死因子-α_TNFSF2作用機(jī)制 - Medchemexpress - MCE中國

1、Product Data SheetRecombinant Human Tumor Necrosis Factor-alpha/TNFSF2Cat. No.: HY-P7058Synonyms: rHuTNF-/TNFSF2; TNF-alpha; Cachectin; DIF; TNFA; Differentiation-inducing factorSpecies: HumanSource: E. coliAccession: P01375Gene ID: 7124Molecular Weight: Approximately 17.3 kDaPROPERTIESAA SequenceV

2、R S S S R T P S D K P V A H V V A N P Q A E G Q L QW L N R R A N A L L A N GV E L R D N Q L V V P S E G L Y L I Y S Q V L F K G Q G C P S T H V L L T H T I S R I A V S Y Q T K V N L L S A I K S P C Q R E T P E G A E A K P W Y E P I Y LG G V F Q L E K G D R L S A E I N R P D Y L D F A E S G Q V Y F G

3、 I I A LBiological Activity The ED50 is 3.3 107 units/mg.Appearance Lyophilized powder.Formulation Lyophilized after extensive dialysis against PBS.Endotoxin Level 0.2 EU/g, determined by LAL method.Reconsititution Reconstitute the lyophilized recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (rH

4、uTNF-/TNFSF2) to 100g/mL using ddH2O or diluted with PBS.Storage & Stability Lyophilized recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (rHuTNF-/TNFSF2) is stored at -20C. Afterreconstitution, it is stable at 4C for 1 week or -20C for longer. It is recommended to freeze aliquots at -20C or - 8

5、0C for extended storage.Shipping Room temperature in continental US; may vary elsewhere.DESCRIPTIONBackground Tumor necrosis factor (TNF-, also known as cachectin) is a strong pro-inflammatory cytokine playing an importantrole in the immune system during inflammation, cell proliferation, differentia

6、tion and apoptosis. TNF- is type IItransmembrane protein that can be expressed in both membrane-bound and secreted forms. Receptors for TNF-aare part of TNF receptor (TNFR) superfamily, with characteristic intracellular segment, a transmembrane domain, andPage 1 of 2 www.MedChemEan extracellular lig

7、and-binding domain. They are type I transmembrane glycoproteins characterized by a cysteinerichmotif (up to 40 aminoacids) repeated 2-6 times in the extracellular N-terminal domain1. Tumor necrosis factor- (TNF-) is a pleiotropic factor exhibiting antitumor effects, alters endothelial barrier functi

8、on, reduces tumor interstitialpressure and mediates immune responses. TNF- is associated with a number of cell signalling events, associatedwith cell necrosis and apoptosis, and is important for resistance to infection and cancer2REFERENCES1. Zelov H, et al. TNF- signalling and inflammation: interac

9、tions between old acquaintances. Inflamm Res. 2013 Jul;62(7):641-51.2. Ren H, et al. Matrix metalloproteinase-mediation of tumor targeting human recombinant tumor necrosis factor- fusion protein. Mol Med Rep. 2015Aug;12(2):2035-42.McePdfHeightCaution: Product has not been fully validated for medical applications. For re