minus-Catechin-gallate-minus-Catechin-3-gallate-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE(-)-Catechin gallateCat. No.: HY-N0356CAS No.: 130405-40-2Synonyms: (-)-Catechin 3-gallate; (-)-Catechin 3-O-gallate分式: CHO分量: 442.37作靶點(diǎn): COX作通路: Immunology/Inflammation儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-

2、20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (226.06 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 2.2606 mL 11.3028 mL 22.6055 mL5 mM 0.4521 mL 2.2606 mL 4.5211 mL10 mM 0.2261 mL 1.1303 mL 2.2606 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn) 請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前?qǐng)先配制澄清的儲(chǔ)備

3、液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.65 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (5.65 mM); Clear solution1/2 Master o

4、f Small Molecules 您邊的抑制劑師www.MedChemEBIOLOGICAL ACTIVITY物活性 (-)-Catechin gallate綠茶茶素中的種微量組分。(-)-Catechin gallate 抑制 COX-1 和 COX-2 活性。IC50 & Target COX-1 COX-2體外研究 (-)-Catechin gallate (CG) directly interacts with DNA oligomers and inhibits the activity of COX-1 and COX-2enzymes, the gene expression

5、of matrix metalloproteinase-9 in macrophage-differentiated HL-60 myeloidleukemia cells, the adipocyte uptake of glucose by the transporter, GLUT4, and the activities of variousproteasomes, i.e., the multicatalytic proteases responsible for the degradation of most cellular proteins. Therelative cytot

6、oxicities of a 3-day exposure to (-)-Catechin gallate are determined for cancerous CAL27 andHSG cells, immortalized epithelioid S-G cells, and normal HGF-1 gingival fibroblasts. The concentration atwhich toxicity (P0.01) initially occur is 25 M (-)-Catechin gallate for S-G cells, 50 M (-)-Catechin g

7、allate forCAL27 cells, 62.5 M (-)-Catechin gallate for HSG cells and 75 M (-)-Catechin gallate for HGF-1 fibroblasts.The calculated neutral red (NR50) values for a 3-day exposure to (-)-Catechin gallate are 58 M for S-Gcells, 62 M for CAL27 cells, 90 M for HSG cells and 132 M for HGF-1 fibroblasts 1

8、.PROTOCOLCell Assay 1 Human tongue squamous carcinoma (CAL27) cells and human salivary gland carcinoma (HSG) cells areused. Individual wells of a 96-well microtiter tissue culture plate are inoculated with 0.2 mL of the growthmedium containing 2104 cells/well for a 1-day exposure, 1.5104 cells/ well

9、 for a 2-day exposure and1104 cells/well for a 3-day exposure to the test agents. After 1 day of incubation, the growth medium isremoved and replaced with exposure medium, with or without varied concentrations of the test agents. Insome studies the cells are coexposed to (-)-Catechin gallate (100, 2

10、00, 300, 400, and 500 M) and 100Units/mL catalase. After 1-3 days of exposure to the test agents, viability is assessed with the neutral red(NR) assay, which is based on the uptake and accumulation of the supravital dye, neutral red (NR) 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Babich H, et al. In vitro cytotoxicity of (-)-catechin gallate, a minor polyphenol in green tea. Toxicol Lett. 2007 Jul 10;171(3):171-80.McePdfHeightCaution: Product has not been f