D-Luciferin-potassium-salt - Dye Reagent - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemED-Luciferin potassium saltCat. No.: HY-12591BCAS No.: 115144-35-9分式: CHKNOS分量: 318.41作靶點: Others作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 12.5 mg/mL (39.26 mM; Need ultrason

2、ic)DMSO : 2.63 mg/mL (8.26 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 3.1406 mL 15.7030 mL 31.4060 mL5 mM 0.6281 mL 3.1406 mL 6.2812 mL10 mM 0.3141 mL 1.5703 mL 3.1406 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 D-Luciferin potassium salt螢光素酶的底物，可

3、催化物發(fā)光昆產(chǎn)典型的黃綠光。體外研究 D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typicalyellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives oranalogues that are substrates or inhibitors of the luciferase fr

4、om the American firefly Photinus pyralis, theenzyme more frequently used in techniques of in vitro and optical imaging 1.1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemE體內(nèi)研究 Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as asubstrate is current

5、ly the most widely employed technique. The total signal intensity is plotted against thetime after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals atfixed time points (5, 10, 15, and 20min) after D-luciferin injection are determined as alternativ

6、es to the peaksignal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to representthe pattern of temporal changes after D-luciferin injection 2.PROTOCOLAnimal Mice 2Administration 2 In vivo BLI is performed using a cooled charge-coupled device camera system

7、(IVIS Imaging System 100) 3,5, 7, 10, 12, 14, 19, 21, 24, and 28 days after the inoculation of HCT116-Luc cells. Mice are injected with 75mg/kg D-luciferin in 100L of phosphate-buffered saline subcutaneously near the scapula and were placedin the light-tight chamber of the imaging system under isofl

8、urane anesthesia. Beginning 5min after injection,dorsal luminescent images with an exposure time of 1s are acquired sequentially at a rate of one image permin until 20min after D-luciferin injection. Data acquisition is continued until 40min postinjection on days 3 or5 and until 25min on day 7, beca

9、use of the prolonged time course of light emission. Binning is 4 and the fieldof view is 15cm.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻 J Leukoc Biol. 2019 Jun 18. Biomed Pharmacother. 2019 Sep;117:109126.See more customer validations

10、on HYPERLINK / www.MedChemEREFERENCES1. Giuseppe Meroni, et al. D-Luciferin, derivatives and analogues: synthesis and in vitro/in vivo luciferase-catalyzed bioluminescent activity.ARKIVOC 2009 (i) 265-288.2. Inoue Y, et al. Timing of imaging after d-luciferin injection affects the longitudinal assessment of tumor growthusing in vivobioluminescence imaging. Int J Biomed Imaging. 2010;2010:471408.McePdfHeightCaution: Product has not been fully