TBK1-IKKε-IN-2-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETBK1/IKK-IN-2Cat. No.: HY-12453CAS No.: 1292310-49-6分式: CHNO分量: 457.52作靶點: IKK作通路: NF-B儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 21.5 mg/mL (46.99 mM; Need ultrasonic and warming)Mass

2、 Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.1857 mL 10.9285 mL 21.8570 mL5 mM 0.4371 mL 2.1857 mL 4.3714 mL10 mM 0.2186 mL 1.0928 mL 2.1857 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 TBK1/IKK-IN-2種雙重 TBK1 和 IKK 抑制劑。IC50 & Target TBK1 IKK0.6 nM (IC50, at 5 M ATP) 3.9 nM

3、 (IC50, at 10 M ATP)體外研究TBK1/IKK-IN-2 (Cmpound #1) inhibits TBK1 biochemical function in Ulight kinase assay at 5 and 250 MATP concentration with IC50s of 0.6 and 2.6 nM, rsespectively. TBK1/IKK-IN-2 inhibits IKK biochemical1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEfunction in Ulight kinase a

4、ssay at 10 M ATP with an IC50 of 3.9 nM. The IC50 of TBK1/IKK-IN-2 in thePanc 02.13 proliferation assay is 5 M 1.PROTOCOLCell Assay 1 Cell proliferation experiments are carried out in a 96-well format (6 replicates), Panc 02.13 cells are plated ata density of 2,000 to 5,000 cells per well. At 24 hou

5、rs following cell seeding, the cells are treated with the toolinhibitor titrations (e.g., TBK1/IKK-IN-2, 1 nM, 10 nM, 100 nM, 1 M and 10 M) for 4 days at 37C and thenassayed by using the ATP CellTiter-Glo luminescent cell viability assay 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Muvaffak A, et al. Evaluating TBK1 as a therapeutic target in cancers with activated IRF3. Mol Cancer Res. 2014 Jul;12(7):1055-66.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 400-820-