roche免疫產(chǎn)品原料簡介剖析.課件

1、免疫產(chǎn)品簡介picture placeholder第1頁，共16頁。抗體銷量最好的抗體：CKMB、DD、Ferr、Insulin、E2。Roche單抗為凍干品，建議用0.9%的生理鹽水溶解，溶解濃度為10mg/ml。溶解液的儲存條件： 1 周 (+2 to +8C), 12 周 (-25 to -15C). 請避免反復(fù)凍融。第2頁，共16頁?？贵w腫瘤：項目抗體批號備注CEAMABTU2 IgG MABM-TU3 IgG 11 353 713 103 10 777 498 103 鼠源單抗凍干AFPMABM-LJ738 IgG MABM-TU11 IgG 11 492 101 103 11 49

2、2 080 103 鼠源單抗凍干FerrMABM-3.170 IgG MABM-4.184 IgG 11 547 089 103 11 547 119 103 鼠源單抗凍干第3頁，共16頁。抗體內(nèi)分泌：項目抗體批號備注LHMABM-11412 IgG MABM-2.406 IgG 11 547 925103 11 547 038103 鼠源單抗凍干FSHMABM-1.303 IgG MABM-W3 IgG 11 493 540103 11 493 531103 鼠源單抗凍干HCGMABM-INN22 IgG MABM-INN2 IgG 11 812 564103 03 116 263103 鼠

3、源單抗凍干PRLMABM-C4E4 IgG MABM-H12G10 IgG 11 458 701103 11 027 689103 鼠源單抗凍干E2PAKK IgG 05 344 255 103兔源多抗液體第4頁，共16頁?？贵w甲功：項目抗體批號備注TSH MABM-1.20 IgG MABM-A8 IgG 10 767 7781 03 11 367 978 103 鼠源單抗凍干T3PABS IgG 10 907 332 103 羊源多抗凍干T4PABS IgG 10 767 794 103 羊源多抗凍干第5頁，共16頁?？贵w其他：項目抗體批號備注Insulin MABM-ST3 IgG MA

4、BM-BM1 IgG 12 208 750 103 12 208 725 103 鼠源單抗凍干CK-MB MABM-7.4.5 IgG MABM-6.12.47 IgG11 719 815 103 11 719 823 103 鼠源單抗凍干D-DimerMABM-1.2.57 IgG MABM-2.1.16 IgG12 156 903 103 12 045 206 103 鼠源單抗凍干IgEMABM-323 IgG MABM-7H8 IgG 11 543 393 103 11 988 204 103 鼠源單抗凍干CRPPABS IgG 11 888 714 103羊源多抗液體第6頁，共16頁。

5、酶和底物ALP底物項目抗體批號備注ALPAlkaline Phosphatase, EIA Grade10 556 602 103液體酶活3000u/mgALPAlkaline Phosphatase, recombinant, highly active03 137 031 103液體，酶活7000u/mg糖含量20-30%ALPAlkaline Phosphatase, recombinant, highlyactive, carbohydrate reduce03 535 452 103液體，酶活7000u/mg近天然，糖含量5-10%項目抗體批號備注pNPP4-Nitrophenyl

6、Phosphate10 004 847 103結(jié)晶粉末BCIP5-Bromo-4-chloro-3-indolyl-phosphate10 760 978 103結(jié)晶粉末第7頁，共16頁。酶和底物POD底物項目抗體批號備注PODPeroxidase (POD), EIA Grade10 815 462 103凍干酶活225u/mgPODPeroxidase (POD), Grade I10 121 606 103凍干酶活250u/mgPODPeroxidase (POD), Grade II11 378 783 103凍干酶活200u/mgPoly PODPoly POD, EIA Grade

7、11 578 545 103凍干酶活600u/mg項目抗體批號備注TMB3,3,5,5-Tetramethylbenzidine10 203 700 103結(jié)晶粉末第8頁，共16頁。放大系統(tǒng)名稱英文名稱批號備注鏈霉親和素 Streptavidin,recombinant11 520 679 103凍干生物素D-Biotin-N-hydroxysuccinimide ester10 734 250 103白色干粉，短鏈生物素D-Biotinoyl-aminocaproic acid-N-hydroxysuccinimide ester11 003 933 103白色干粉，長鏈鏈霉素包被磁珠Str

8、eptavidin Magnetic Particles11 636 502 103懸濁液磁珠直徑2um左右裸珠第9頁，共16頁。干擾消除蛋白非特異性干擾第10頁，共16頁。非特異性干擾消除蛋白Bovine IgG PABB-IgG (DE)Cat. No. 11293621 Interference reduction in assay system working with polyclonal bovine IgG through blocking of e.g. antibody solid phase interference Basic interference eliminati

9、on. Specials: IgG fraction from bovine plasma, purified by DE chromatography with 5 % aggregated IgGBSA VCat. No. 10738328 Saturation of unoccupied binding sites on the solid phase Basic interference elimination. Specials: Contains no detectable IgGsSheep IgG PABS-IgG (DE)Cat. No. 10717606 Interfere

10、nce reduction in assay system working with polyclonal sheep IgG through blocking of e.g. antibody solid phase interference Basic interference elimination.Rabbit IgG PABK-IgG (DE)Cat. No. 10912280 Interference reduction in assay system working with polyclonal rabbit IgG through blocking of e.g. antib

11、ody solid phase interference Basic interference elimination.第11頁，共16頁。非特異性干擾消除蛋白BPLA I + IVBovine Plasma AlbuminCat. Nos. 1172 6536 1172 6544 0353 5240 Avoidance of non-specific adsorption or antibody interactions for more complex applications with higher demands on sensitivity. Specials: High purit

12、y plus pH 4.5 treatmentType I: purity HPLC 95 %, low content of steroids, lipids and haptens Type IV: purity HPLC 95 %, Type IV NEW !: purity HPLC 95 %, Protease-free ! 2 more production steps compared to old version.Poly BSA I + IICat. Nos.11866737 + 11816438Due to their own polyvalence they show o

13、ptimal protection against polyvalent interference (e.g. in infectious disease assays). The negative charge also avoids hydrophobic interactions.Specials: Very efficient background signal reduction.Type I - acetylated; diameter 30 nmType II succinylated; diameter 40 nm It is recommended to combine a

14、mono- and a polyvalent reagent !The total amount of non specific IEP is recommended to be at 2-5%.第12頁，共16頁。特異性干擾-MAK33 家族單體 IEPsNote: The use of a framework-specific IEP is covered by Roche patent.MAK33 IgG (lyo) Cat. No 1200 941 - monoclonal IgG1 antibody with defined specificity - for test formul

15、ations working with intact IgG1 - mainly for elimination of monomeric and specific interferenceIEP-FrameworkCat. No. 03369846 - for elimination of monomeric and highly specific Interference against the framework regions - caused by highly specific and mostly Mab-therapy-induced HAMAS - developed by

16、comparing the amino acid-sequences of different assay antibodies - use identical immunoreactive amino acid sequences in the framework regions of the interference eliminating reagent as in the test antibodies. - recommended for tumor marker, thyroid and cardiac assays.第13頁，共16頁。特異性干擾-MAK33 家族多聚體IEPs

17、Note: The use of polymerized IEPs is covered by Roche patent.MAK33-IgG1/IgG1 Poly (liquid) Cat. No 1939 661 polymerized version of MAK33 (DSS linker chemistry) for test formulations working with intact IgG1 higher efficiency concerning HAMA interference type 1 (spontaneous HAMAs) mainly for eliminat

18、ion of polymeric and more unspecific interference.MAK33-IgG1/Fab1 Poly (lyo) Cat. No 1368 338 recommended for test systems working with Fab conjugates comparable characteristics as the a.m. MAK33-IgG/IgG1 poly in addition it covers Fab Neo-epitopes mainly for elimination of polymeric and more unspecific interference.Poly MAK IgG2b/Fab2a (lyo) Cat. No 1355 830 recommended for tes