簡介WatersUPLC故障排除專題培訓(xùn)課件

1、Waters UPLC Troubleshooting Training AgendaACQUITY UPLC Family ：UPLC & H-ClassWaters UPLC SystemUPLC Solvent Management (BSM)UPLC Sample Management (SM)Waters ACQUITY H-Class SystemQuaternary Solvent Manager (QSM) Sample Manager-Flow Through Needle (SM-FTN)Waters UPLC Detector Troubleshooting Traini

2、ngWaters UPLC PDA and PDAeTUVFLRELSDOpen DiscussionACQUITY UPLC FamilyACQUITY UPLC SystemACQUITY UPLC SystemDetectors:Optical and/or Mass SpecTunable UV or Photodiode ArrayOptimized flow cell for UPLCHigh speed data samplingColumn Manager:Innovative pivot designPositions column to detectorE-Cord con

3、nectionSample Manager:Low dispersion XYZZ FormatFast cycle timesLow carryoverPlates and/or vialsOptional Sample OrganizerBinary Solvent Manager:High pressure blendingBinary gradientsFour solvent choicesOn-line degassingLow dispersion designUPLC pressure capabilitiesSample Organizer: (option)Expands

4、capacity (22/15/8)Shuttles plate feedHeated/chilledSystem Considerations:Small FootprintRedesigned tubing and fittingsConsolidated waste managementIntegrated system diagnosticsConnections Insight remote diagnosticsA1 A2B1B2WasteFluidics and ConnectionsTubing management Chromatographic solvents:A1/A2

5、, B1/B2Two Sample Manager wash solventsSeal WashCommon fittings and tools for usersColumn inlets/outlets and fittings Finger tight, turn (use 1/4” wrench)Dedicated tubing3 piece compression screw and ferruleSmall footprint 13” wide24.25” with Sample OrganizerUPLCSolvent ManagerACQUITY UPLC System So

6、lvent Delivery 15 K psi performanceAccurate and precise flow ratesAccurate and precise compositionLow delay volume for gradient applications5% Ethyl AcetateToluene Chlorinated solvents: (Trichlorobenzene) UPLCSample ManagerSample Manager Fluidics Tray Pressure Transducer Valve Manifold Drive Gears W

7、ash SyringesWeak Wash Strong WashMetering Syringe Drive Assembly Metering Syringe Valve3 Way Rotary ValveMetering Syringe AssemblySample Syringe50 uL volume PN 410001348100 L volume PN 700002570250 L volume PN 410001347for use with 50 L loopColumn Heating CompartmentACQUITY In-Line Filter18Column In

8、-Line Filter AssemblyColumn In-Line Filter AssemblyColumn HeatingSample Manager Flow PathAvailable Loops DescriptionP/NStatus1 L Loop430001516Optional2 L Loop430001264Optional5 L Loop430001233Optional10 L Loop430001326Standard20 L Loop430001320Optional 50 L Loop430001325Optional100 L Loop170001792(K

9、it) Optional250 L Loop176001780(Kit) OptionalVolume Configuration MenuBefore running the Characterize Needle and Loop Volume function, make sure the proper volumes are configured in the Volumes Configuration menu. The above values are standard for currently shipping ACQUITY system.Recommended Inject

10、ion Volumes,Loop Sizes and Injection ModesLoop Volume (uL)Partial Loop w/Needle Overfill Mode Recommended sample injection volume range (uL)Partial Loop (Pressure Assist Mode) Recommended sample injection volume range (uL)Full Loop Mode Recommended sample injection volume (uL0Automatic overfill fact

11、or1uL0.2 to 0.75Not Recommended1.05.82uL0.4 to 1.5Not Recommended2.05.65uL1.0 to 3.8Not Recommended5.0510uL2.0 to 7.52.0 to 5.010.0420uL4.0 to 15.04.0 to 10.020.0350uL10.0 to 37.510.0 to 25.050.02100uL20.0 to 75.020.0 to 50.0100.02250uL40.0 to 150.040.0 to 125.0250.01.5Recommended PLNO injection vol

12、ume 20% to 75% of loop volumeRecommended Partial Loop Injection 20% to 50% of loop volumeACQUITY H-Class SystemQSMQuaternary Solvent Manager SM-FTNSample Manager-Flow Through Needle CH-AHigh Temperature Column HeaterActive Pre-Heating DetectorsTUV, PDA ePDA, FLR, ELSDThe ACQUITY H-Class System consi

13、sts of the following:Quaternary Solvent Manager (QSM)Four solvent low pressure gradient mixing Supports UPLC PressuresLow gradient delay volumeSM-FTN (Sample Manager-Flow Through Needle)One injection mode- direct inject typeMuch simpler- Just specify injection volumeExcellent recovery and precisionC

14、H-AHigh temperature column heater with active pre-heatingACQUITY H-Class SystemQSM Fluidics-Front ViewPrimary HeadAccumulator HeadVent Valve100ul MixerGradient Proportioning Valve PairsLow PressureManifoldDegasser ChambersSeal Wash PumpQSM Console Top Level MenuACQUITY H-Class SystemSMDI Front Panel

15、 FluidicsMetering Syringe ValveCheck Valve and UnionInject ValveWash Solvent Inlet Line (not degassed)Leak Sensor ConnectionsSample Metering SyringeSDMI Inject Valve ConnectionsTo Check ValveTo CH-A and ColumnStationary Side TubeFrom PumpTo Pressure TransducerTo Sample NeedleSample CompartmentPower

16、and Status LEDsPlate Selector SwitchRotary Tray(Plate Holder)Magnetic Door SensorPower SwitchSM-FTN Interactive Display MenuCH-AImproved Door GasketAdded Tubing Gasket (top and bottom)For 3rd party Mass Spec at left side ofACQUITY Stack.Allows use of existingPassive Pre-heaterNo wires to connectActive Pre-heaterInstalling the Active Pre-HeaterGold Plated Compression ScrewPEEK FerruleStainless Steel Cap NutStainless Steel Tubing (with brazed on collet)Installing the Active Pre-HeaterUPLCDetectorsDetectorsSupports all current ACQUITY detec