簡(jiǎn)介Waters-UPLC故障排除課件

1、2022/10/1112022/10/111Waters UPLC Troubleshooting Training 2022/10/112Waters UPLC Troubleshooting TrAgendaACQUITY UPLC Family ：UPLC & H-ClassWaters UPLC SystemUPLC Solvent Management (BSM)UPLC Sample Management (SM)Waters ACQUITY H-Class SystemQuaternary Solvent Manager (QSM) Sample Manager-Flow Thr

2、ough Needle (SM-FTN)Waters UPLC Detector Troubleshooting TrainingWaters UPLC PDA and PDAeTUVFLRELSDOpen Discussion2022/10/113AgendaACQUITY UPLC Family ：UPLACQUITY UPLC Family2022/10/114ACQUITY UPLC Family2022/10/114ACQUITY UPLC System2022/10/115ACQUITY UPLC System2022/10/115ACQUITY UPLC SystemDetect

3、ors:Optical and/or Mass SpecTunable UV or Photodiode ArrayOptimized flow cell for UPLCHigh speed data samplingColumn Manager:Innovative pivot designPositions column to detectorE-Cord connectionSample Manager:Low dispersion XYZZ FormatFast cycle timesLow carryoverPlates and/or vialsOptional Sample Or

4、ganizerBinary Solvent Manager:High pressure blendingBinary gradientsFour solvent choicesOn-line degassingLow dispersion designUPLC pressure capabilitiesSample Organizer: (option)Expands capacity (22/15/8)Shuttles plate feedHeated/chilledSystem Considerations:Small FootprintRedesigned tubing and fitt

5、ingsConsolidated waste managementIntegrated system diagnosticsConnections Insight remote diagnostics2022/10/116ACQUITY UPLC SystemDetectors:CA1 A2B1B2WasteFluidics and ConnectionsTubing management Chromatographic solvents:A1/A2, B1/B2Two Sample Manager wash solventsSeal WashCommon fittings and tools

6、 for usersColumn inlets/outlets and fittings Finger tight, turn (use 1/4” wrench)Dedicated tubing3 piece compression screw and ferruleSmall footprint 13” wide24.25” with Sample Organizer2022/10/117A1 A2B1B2WasteFluidics and CoUPLCSolvent Manager2022/10/118UPLCSolvent Manager2022/10/11ACQUITY UPLC Sy

7、stem Solvent Delivery 15 K psi performanceAccurate and precise flow ratesAccurate and precise compositionLow delay volume for gradient applications5% Ethyl AcetateToluene Chlorinated solvents: (Trichlorobenzene) 2022/10/1114Recommended Solvents ListACQUIUPLCSample Manager2022/10/1115UPLCSample Manag

8、er2022/10/111Sample Manager Fluidics Tray Pressure Transducer Valve Manifold Drive Gears Wash SyringesWeak Wash Strong WashMetering Syringe Drive Assembly 2022/10/1116Sample Manager Fluidics Tray Metering Syringe Valve3 Way Rotary ValveMetering Syringe AssemblySample Syringe50 uL volume PN 410001348

9、100 L volume PN 700002570250 L volume PN 410001347for use with 50 L loop2022/10/1117Metering Syringe ValveMeteringColumn Heating Compartment2022/10/1118Column Heating Compartment2022ACQUITY In-Line Filter192022/10/1119ACQUITY In-Line Filter192022/1Column In-Line Filter Assembly2022/10/1120Column In-

10、Line Filter AssemblyColumn In-Line Filter Assembly2022/10/1121Column In-Line Filter AssemblyColumn Heating2022/10/1122Column Heating2022/10/1122Sample Manager Flow Path2022/10/1123Sample Manager Flow Path2022/1Available Loops DescriptionP/NStatus1 L Loop430001516Optional2 L Loop430001264Optional5 L

11、Loop430001233Optional10 L Loop430001326Standard20 L Loop430001320Optional 50 L Loop430001325Optional100 L Loop170001792(Kit) Optional250 L Loop176001780(Kit) Optional2022/10/1124Available Loops DescriptionP/Volume Configuration MenuBefore running the Characterize Needle and Loop Volume function, mak

12、e sure the proper volumes are configured in the Volumes Configuration menu. The above values are standard for currently shipping ACQUITY system.2022/10/1125Volume Configuration MenuBeforRecommended Injection Volumes,Loop Sizes and Injection ModesLoop Volume (uL)Partial Loop w/Needle Overfill Mode Re

13、commended sample injection volume range (uL)Partial Loop (Pressure Assist Mode) Recommended sample injection volume range (uL)Full Loop Mode Recommended sample injection volume (uL0Automatic overfill factor1uL0.2 to 0.75Not Recommended1.05.82uL0.4 to 1.5Not Recommended2.05.65uL1.0 to 3.8Not Recommen

14、ded5.0510uL2.0 to 7.52.0 to 5.010.0420uL4.0 to 15.04.0 to 10.020.0350uL10.0 to 37.510.0 to 25.050.02100uL20.0 to 75.020.0 to 50.0100.02250uL40.0 to 150.040.0 to 125.0250.01.5Recommended PLNO injection volume 20% to 75% of loop volumeRecommended Partial Loop Injection 20% to 50% of loop volume2022/10

15、/1126Recommended Injection Volumes,ACQUITY H-Class SystemQSMQuaternary Solvent Manager SM-FTNSample Manager-Flow Through Needle CH-AHigh Temperature Column HeaterActive Pre-Heating DetectorsTUV, PDA ePDA, FLR, ELSD2022/10/1127ACQUITY H-Class SystemQSMSM-FTThe ACQUITY H-Class System consists of the f

16、ollowing:Quaternary Solvent Manager (QSM)Four solvent low pressure gradient mixing Supports UPLC PressuresLow gradient delay volumeSM-FTN (Sample Manager-Flow Through Needle)One injection mode- direct inject typeMuch simpler- Just specify injection volumeExcellent recovery and precisionCH-AHigh temp

17、erature column heater with active pre-heatingACQUITY H-Class System2022/10/1128The ACQUITY H-Class System conQSM Fluidics-Front ViewPrimary HeadAccumulator HeadVent Valve100ul MixerGradient Proportioning Valve PairsLow PressureManifoldDegasser ChambersSeal Wash Pump2022/10/1129QSM Fluidics-Front Vie

18、wPrimaryQSM Console Top Level Menu2022/10/1130QSM Console Top Level Menu2022ACQUITY H-Class System2022/10/1131ACQUITY H-Class System2022/10/2022/10/11322022/10/1132SMDI Front Panel FluidicsMetering Syringe ValveCheck Valve and UnionInject ValveWash Solvent Inlet Line (not degassed)Leak Sensor Connec

19、tionsSample Metering Syringe2022/10/1133SMDI Front Panel FluidicsMeterSDMI Inject Valve ConnectionsTo Check ValveTo CH-A and ColumnStationary Side TubeFrom PumpTo Pressure TransducerTo Sample Needle2022/10/1134SDMI Inject Valve ConnectionsTSample CompartmentPower and Status LEDsPlate Selector Switch

20、Rotary Tray(Plate Holder)Magnetic Door SensorPower Switch2022/10/1135Sample CompartmentPower and StSM-FTN Interactive Display Menu2022/10/1136SM-FTN Interactive Display MenCH-AImproved Door GasketAdded Tubing Gasket (top and bottom)For 3rd party Mass Spec at left side ofACQUITY Stack.Allows use of e

21、xistingPassive Pre-heaterNo wires to connectActive Pre-heater2022/10/1137CH-AImproved Door GasketAdded Installing the Active Pre-Heater2022/10/1138Installing the Active Pre-HeatGold Plated Compression ScrewPEEK FerruleStainless Steel Cap NutStainless Steel Tubing (with brazed on collet)Installing the Active Pre-Heater2022/10/1139Gold Plated Compression ScrewPUPLCDetectors2022/10/1140UPLCDetectors2022/10/1140DetectorsSupports all current ACQUITY detectorsPDA and PDAeTUVFLRELSD2022/10/1141DetectorsSupports all current ACQUITY UPLC PDA2022/10/1142A