DGGE條帶多樣性指數(shù)等分析

1、 首先將JPG格式轉(zhuǎn)換為T(mén)IF格式（根據(jù)自己的情況選擇軟件）2、 將TIF格式圖片導(dǎo)入QuantityOne（用打開(kāi)命令即可）3、 點(diǎn)擊Lane命令，出現(xiàn)下拉菜單，點(diǎn)擊FrameLanes（如果命令鍵為灰色，請(qǐng)下載補(bǔ)丁安裝。）FileEditViewImageLaneBandMatchVolumeAnalysisReportsWindowHelp在出現(xiàn)的對(duì)框中填入泳道數(shù)，點(diǎn)擊OK,進(jìn)行調(diào)整，使紅色線(xiàn)條盡量在泳道的中間部位，整體拉動(dòng)lane框架，還可以進(jìn)行單獨(dú)的調(diào)整，在lane命令下的singlelane—adjustlane。4、調(diào)整好lane后，點(diǎn)擊band下的detectbandsFileEditViewImageLaneBandMatchVolumeAnaly&isReportsWindowHelp出現(xiàn)下圖在軟件右方位置，有如下對(duì)話(huà)框，可根據(jù)需要設(shè)置紅色框區(qū)域的屬性（包括密度、寬度等）。SensitivitvLaneWidthMin.DensityNoiseFiltershoulderSens.SizeScaleDetect參DetectBands-SensitivitvLaneWidthMin.DensityNoiseFiltershoulderSens.SizeScaleDetect參DetectBands-口展E圖譜(RawParameterSetName|LecIISmv—|Fl—t|LanesPAIIrOneDetect(**AutoCManualNormalizepYesrNoShadowsPAcceptCRejectBandsPAIIrLimit調(diào)整好了，再根據(jù)實(shí)際情況，手動(dòng)添加刪除調(diào)動(dòng)條帶，采用褐色框的命令。（需要耐心）5、調(diào)整好后，如下圖（僅僅是假設(shè)圖）點(diǎn)擊match下的match命令，會(huì)出現(xiàn)下圖。FileEditViewImageLaneEandMatchVolumeAnalysisReportsWindowHelpFileEditViewImageLaneEandMatchVolumeAnalysisReportsWindowHelp根據(jù)實(shí)際情況，采用match與unmatch調(diào)節(jié)自己的條帶的遷移率的匹配度，這一步最關(guān)鍵，最需要耐心，匹配有問(wèn)題，就用unmatch取消再進(jìn)行match命令，心靜就行。WindowHelp5、match完成后，假如上圖，即可導(dǎo)出一系列reports。WindowHelpFileEditViewImageLaneBandMatchVolumeAnalysisRepots很多，但計(jì)算多樣性指數(shù)的為lanereport，如下圖選擇一些指標(biāo)，點(diǎn)擊report。

)1ExportLan?Report?85818)1ExportLan?Report?85818J8FieldSeparatorsPCommas(1-2-3format)<**Tabs(Excelformat]ExportDestination口FilePClipboardXCancel(J]HelpJ依次點(diǎn)擊紅色框命令，保存txt到指定文件夾下，將txt文件里的數(shù)據(jù)復(fù)制粘貼到excel中，如下圖:

一an已BandBandRelativeAverage-A^—C^D'lumberNumberType#FrontInt sum1340.12498.1551674.5450.0586162.8367490.1662791410.029105.7481674.5450.063152.7622380.1744361560.19115.7181674.5450.0691042.6721410.1846561690.262124.521674.5450.074362.598830.1932517100.327117.3181674.5450.070062.6584090.186247117110.413124.5941674.5450.0744052.5982360.193321118130.484151.8051674.5450.0906542.40070.217634119140.511150.8091674.5450.090062.4072830.216799120210.693148.9221674.5450.0889332.4198740.215206121220.719146.3851674.5450.0874182.4370570.213042125280.835109.8141674.5450.0655782.7245090.178669126250.788140.5541674.5450.0839362.4777050.2079681_ 27260.806140.2031674.5450.0837262.4802050.207658A2440.12395.6481598.2270.0598462.8159750.1685262510.027103.0941598.2270.0645052.7410090.1768092660.182110.6991598.2270.0692642.6698350324113.91598.2270.0712662.6413290.188238217110.41120.7111598.2270.0755282.5832510.195108218130.486141.4851598.2270.0885262.4244560.214628219140.512142.3981598.2270.0890972.4180240.21544220180.625150.5481598.2270.0941972.3623680.222528221210.696130.5911598.2270.081712.5045S0.204649222220.719130.0491598.2270.0813712.5087390.204138223250.785128.0241598.2270.0801042.5244320.202217224280.836103.5511598.2270.0647912.7365860.177307225260.81127.5291598.2270.0797942.5283060.2017442.將第一條泳道的averageint進(jìn)行求和，上圖A列；用averageint值比上A列計(jì)算的求和，得出B列數(shù)值；對(duì)B列數(shù)值求自