單細胞DNA甲基化分析

單細胞DNA甲基化分析occursatthecarbon-5positionofcytosineresidueoftheCpGdinucleotideRelatedtoX-chromosomeinactivation,imprinting,andmaintenanceofchromosomalstabilityschizophrenia,bipolardisorder,Alzheimer’sdisease,diabetes,atherosclerosis,cancer,majorpsychosis,lupusandParkinson’sdisease分離單細胞單細胞DNA甲基化甲基化分析分離單細胞流式細胞分析酶解法微流體設(shè)備激光捕獲顯微切割技術(shù)用毛細管等器具手動獲取甲基化分析重亞硫酸鹽胞嘧啶尿嘧啶PCR富集測序分析那為何單細胞DNA甲基化分析直到近些年才成為研究熱點了呢？enzyme-basedsingle-cellmethylationassay(RSMA)論文的結(jié)構(gòu)和主要內(nèi)容全文總結(jié)默克密理博(MerckMillipore)開發(fā)的一種試劑盒：(CpGenome?TurboBisulfiteModificationKit)提高實驗速度保證檢測的靈敏度轉(zhuǎn)化效果好默克密理博的秘密就是他們的一款受專利保護的處理試劑，能在很短時間內(nèi)將未甲基化的胞嘧啶徹底轉(zhuǎn)化參考文獻MartinKantlehner,RolandKirchner.Ahigh-throughputDNAmethylationanalysisofasinglecell.NucleicAcidsResearch,2011,Vol.39,No.7e44;C.Lorthongpanich,L.F.Cheow,S.Balu,S.R.Quake,B.B.Knowles,W.F.Burkholder,D.Solter,D.M.Messerschmidt.Single-cellDNA-methylationanalysisrevealsepigeneticchimerisminpreimplantationembryos.Science,341(2013),pp.1110–1112;Eun-JoonLee,JunfengLuo.Analyzingthecancermethylomethroughtargetedbisulfitsequencing.CancerLett.2013November1;340(2);SébastienASmallwood,HeatherJLee.single-cellgenome-widebisulfitesequencingforassessingepigeneticheterogeneity.naturemethods,2014,VOL.11,NO.8,pp.817–820;MatthiasFarlik,NathanC.SheffieldSingle-CellDNAMethylomeSequencingandBioinformaticInferenceo