NBD-14270-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemENBD-14270Cat.No.:HY-139989CASNo.:2411819-82-2分?式:C??H??F?N?O?S分?量:425.43作?靶點(diǎn):HIV作?通路:Anti-infection儲(chǔ)存?式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(235.06mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.3506mL11.7528mL23.5056mL5mM0.4701mL2.3506mL4.7011mL10mM0.2351mL1.1753mL2.3506mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(5.88mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(5.88mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(5.88mM);ClearsolutionBIOLOGICALACTIVITY?物活性NBD-14270，?種吡啶類(lèi)似物，?種有效的HIV-1進(jìn)?拮抗劑，對(duì)50株HIV-1假型病毒的IC50為89nM。NBD-14270與HIV-1gp120結(jié)合并顯?出強(qiáng)?的抗病毒活性(EC5050>100μM)。IC50&TargetHIV-1體外研究NBD-14270hasanti-HIV-1activity(IC50=0.16μM)andCytotoxicity(CC50=109.3μM)insingle-cycle(TZM-blCells)assay[1].NBD-14270doesnotinducetoxicityintheU87-CD4-CXCR4celllineatthedosesusedforthisassay[1].REFERENCES[1].FrancescaCurreli,etal.PreclinicalOptimizationofgp120EntryAntagonistsasanti-HIV-1AgentswithImprovedCytotoxicityandADMEPropertiesthroughRationalDesign,Synthesis,andAntiviralEvaluation.JMedChem.2020Feb27;63(4):1724-1749.[2].NatalieLosada,etal.HIV-1gp120AntagonistsAlsoInhibitHIV-1ReverseTranscriptasebyBridgingtheNNRTIandNRTISites.JMedChem.2021Nov25;64(22):16530-16540.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.