維藥洋甘菊中黃酮類成分

維藥洋甘菊中黃酮類成分

1.細(xì)胞系統(tǒng)的選擇馬爾卡西翁。（蒙馬西翁）的一個(gè)類別是一個(gè)計(jì)劃，它是一個(gè)自給自足的例子。它是西蒙布魯斯塔納爾的一個(gè)區(qū)域，也是霍納斯。2.母乳喂養(yǎng)2.1.n.au7.NMRspectrawererecordedonaBrukerAV-600NMRspectrometer(BrukerBioSpin,Switzerland)usingTMSasaninternalstandard.EI-MSdatawereobtainedonaQP2010UltralGC-MS(ShimadzuCorporation,Japan)viadirectinjection.ESI-MSdatawereobtainedonanAgilent6130SQ(AgilentTechnologies,America).Semi-preparativeHPLCwasperformedonanLC3000instrument(ChuangxinTonghengCompany,China)usingaC2.2.主要計(jì)劃設(shè)計(jì)參數(shù)Theair-driedwholeherbofM.chamomillawaspurchasedfromTachengCounty,XinjiangUygurAutonomousRegion,China.TheplantmaterialswereidentifiedasM.chamomillaL.byProf.ChunshengLiu,andthespecimenwasdepositedattheInstituteofChineseMateriaMedica,ChinaAcademyofChineseMedicalSciences(Beijing,China).2.3.fra植物基因組織fracevirdeaThecrudepowderedwholeherbofM.chamomilla(19kg)wasextractedwith95%EtOH.Afterremovalofthesolventbyevaporationinreducedvacuum,theresiduewassuspendedinHThePetfractionwassubjectedtopressurereducingsilicagelcolumnchromatographyandelutedwithPet–EA(9:1–0:1,v/v)toget12fractions(Fr.1–Fr.12).Fr.4(Pet–EA,8:2,v/v)wassubjectedtocolumnchromatographyonMCIgelandelutedwithMeOH–HTheEAfractionwassubjectedtopressurereducingsilicagelcolumnchromatographyandelutedwithPet–EA(9:1–0:1,v/v)andMeOH–H3.Identification3.1.ApigeninYellowpowder(acetone);EI-MSm/z270[M]3.2.加蘭戈Yellowpowder(methanol);EI-MSm/z270[M]3.3.檢察官辦公室Yellowpowder(methanol);EI-MSm/z286[M]3.4.cid-relactoryYellowpowder(methanol).Bythecomparisonofcompound4andthereferencestandardkaempferolthroughthemethodofTLC,therelativeshiftandthecolorwerebasicallythesameaftersprayingofvanillinof10%sulfuricacidsolution.Bythecomparisonofcompound4andthereferencestandardkaempferolthroughthemethodofHPLC,theretentiontimeandUVspectrawerebasicallythesame.Therefore,compound4wasidentifiedaskaempferol.3.5.QuercetinYellowpowder(methanol).EI-MSm/z302[M]3.6.HispidulinYellowpowder(methanol).EI-MSm/z310[M]3.7.6-metho公車Yellowpowder(methanol).EI-MSm/z316[M]3.8.EupafolinYellowpowder(acetone).EI-MSm/z316[M]3.9.3-methyl峽谷Yellowpowder(acetone).3.10.埃德曼Yellowneedlecrystal(acetone).EI-MSm/z314[M]3.11.5、7、4二棕櫚樹-3、6-迪莫頓Yellowpowder(methanol).EI-MSm/z330[M]3.12.聯(lián)合頁(yè)Yellowpowder(methanol).(+)ESI-MSm/z477[M+H]3.13.7-o--d-glu-加拉迪Yellowpowder(methanol).(+)ESI-MSm/z465[M+H]3.14.esi-ms-1.3.3index,關(guān)于“以負(fù)共聚”的pcr-pcr的國(guó)際分布Lightyellowpowder(methanol).(+)ESI-MSm/z515[M-H]ItwasidentifiedasisochlorogenicacidBbytheNMRdataaccordingtotheliteraturerecord3.15.esi-ms-z515[m-h]Lightyellowpowder(methanol).(+)ESI-MSm/z515[M-H]ItwasidentifiedasisochlorogenicacidCbytheNMRdataaccordingtotheliteraturerecord3.16.4-hydrolybenzonacidWhiteso