脊神經(jīng)節(jié)微血管內(nèi)皮細(xì)胞陰離子微區(qū)的分布特點(diǎn)、生物學(xué)特性及損傷的影響

1、脊神經(jīng)節(jié)微血管內(nèi)皮細(xì)胞陰離子微區(qū)的分布特點(diǎn)、生物學(xué)特性及損傷的影響the distribution characteristics, biological properties and effects of the microvascular endothelial cells in microvascular endothelial cellsupdate date: 2010-07-03 zhou yue liu zhengzunhong xiao yuan【abstract 】 objective to study the spinal ganglion (drg) blood anio

2、n micro zone - ncrvc barrier microvascular cndothclial cells (amd) distribution, biological characteristics and rclationship with the vascular permeability increase after injury. methods the healthy home rabbit 42 was divided into surgical control group, mechanical comprcssion group, inflammatory in

3、jury group. the method was observed by using the cationic colloidal gold (ccg) probe and the scries of cnzymes. the result (1) drg vascular endothelial cells have a polarized region of anionic distribution; (2) the main constituent of the molecules of the drg vascular cndothclial cells conclusion dr

4、g of protein in plasma vascular cndothclial cells still have negative ion permeability barrier effect, anionic drg microvascular cndothclial cells damage will result in the barrier of the microvascular permeability increased significantly.keywords the spinal nerve section damages the cationic gold o

5、f the endothelial cells of the vascular endothelial cellsstructural and molecular character!sties of the effects of the company and injurics on anionic microdomains on endoncurialcapillaries in dorsal root gangliaziiou make, liu zheng - jin mei fang - rui, et al., dept of orthopedics, the second aff

6、iliated hospital, the third he medical university, chongqing, 400037abstract aim to explore the structural and molecular characteristics of anionic microdomains on the endoneurial capillaries, and the relation with the increased vascular permeability following dorsal root ganglia (drg) injury. metho

7、ds forty-two rabbits were randomly divided into the control group, the mechanical compression group and the inflammatory injury group cationic colloidal gold (ccg) probe and various enzymes digestion on ccg labelling were taken for the study. results (1) there were anionic microdomains and distinct

8、charge polarization in the endotheliocytes of drg between luminal and albuminal fronts(2) the sialoglycoconjugates were the major components of the negatively charged microdomains in dgr conclusion the endoneurial capillaries in drg may become a barrier, especially be able to limit the plasma protei

9、n extravasation. inflamniqtory injury can reduce largely the anionic charges on the luminal plasmalemma, destroy the distribution of anionic microdomains and enhance the vascular permeability significantly.key words dorsal root ga.nglia，s endotheliocyte microdomain cationic colloidal goldthe author

10、uses the series of cationic colloidal gold (ccg) probe and enzyme digestion after embedding methods of tag, explore spinal ganglion microvascular endothelial cells (drg) blood 一 nerve barrier anion micro zone (amd) distribution, biological characteristics and relationship with the vascular permeabil

11、ity increase after injury.materials and methodsfirst, animal and group: healthy home rabbit 42, male and female. weigh 2. 2 to 3 1 kg. randomly divided into three groups: group a (control group, numbet of rabbits 二 7); group b (acute mechanical compression group, rabbit number 二 7); group c (inflamm

12、atory injury group,rabbit 二 28).model replication: 3% pentobarbital sodium (0.5 1.0 ml/kg) ears intravenous anesthesia, regular disinfection with sterile towels, midline incision after lumbar di ministry, remove the l5 7 spines and lamina, surgical microscope revealed bilateral drg5 and drg6 careful

13、ly. the left side is the side of the injury, and the right side is the non-damaged side the group b used two microsurgical blood vessels (2mm in width, 3mm in width, 45g in pressure), and the left drg5 and drg6 group c by 4-0 chromic catgut, cut into 0. 5 cm long, take 4 6, along the left drg5 and d

14、rg6 arrange placed around the vertical axis, fixed and sterile surgical bone wax, 1 ' 2 layers of gelatin sponge cover on the surface of the catheter, suture wounds, breeding housed separately. the group a showed and treated the same group b and group c, and group a was treated as a control grou

15、p, while the group b and group c had the drg5 and drg6 on the right side of the group as the control group.preparation of ccg probe and electron microscopy the preparation of ccg: reference to the 1 methods such as skutelsky. first of all, the colloidal gold with 0. 1 mol/l k2c03 (cg, 8 12 nm, sigma

16、 company) to ph7 0, determined by polymer flocculation precipitation 2 - l - lysine (pl, mw > 300000, sigma company) used to stabilize the quantity of different concentrations of cg, dissolved the cg and in pairs of steaming water pl quickly blend crude pl - g compounds, 4 °c overspeed centr

17、ifugal 10000 20000 g / 45 min. will go to clear liquid absorption, 0. 01 mol/l pbs (ph7 2) add to the precipitation of plg, 4 °c.specimen and microscopy: 2. frozen biopsy in 0. 1 mol/l ammonium chloride effects after 3 hours, add have finished preparation of ccg (using 0. 1 mol/l pbs, ph2. 0, c

18、ontaining 0. 1% bsa diluted into 1:40) incubation 3 6 hours at room temperature, and then use 0. 01 mol/l pbs (ph7. 2) rinsed three times, 2% 0s04 fixed 3 hours, the conventional electron microscopy embedding, transmission electron microscope the ccg is high electron density particle in the electron

19、 microscope the result analysis uses the image analyzer to calculate the number of ccg number (ccg number/mu m2) per square micron in different partsthe biological characteristics of the molecules in the ionic microregions: follow the process of the digestion and embedding of the bimonase by lawrens

20、on. tissue samples, fixation, dehydration, and resin embedding thin nickel net slice, slice was placed in a box of wet, each section respectively, add 1 drops enzyme digestive juices (enzyme digestion group) or buffer (not digest group), 37 °c incubation 1 2 hours this is the pancreatic enzyme

21、2mg/ml pbs, ph7.3. papaya protease 2mg/ml acetate buffer, pl 16 1. ileparin enzyme, 160u/ml pbs, ph7.3. neuraminidase lou/ml buffer, ph5o. after an hour of digestion, drip plus pbs (0. 01 molar, ph 7. 4) rinse, and then add ccg to each slice and incubate at room temperature for 1 2 hours finally, wi

22、th 0.01 mol/l, ph7.4 pbs rinsing, the dioxy of acetic acid and the lead of the citric acid, the electron microscope observed ccg with high electron density particlesstatistics processingthe data of the experimental results are in 486 / dx266 type microcomputer using microsoft excel statistical proce

23、dure of 5. 0 line sample single factor and multiple factors analysis of variance, significant t test, the results to mean + / - standard deviation (+ / - s) said.the results ofthe distribution characteristics and damage of ccg in drgdrg cavity on the surface of the membrane microvascular endothelial

24、 cells within the cytoplasm membrane, scattered and small ccg high electron density distribution in the particles, the cavity has ccg distribution at the membrane surface, but the cavity on the surface of the membrane ccg particles more obvious than the cavity surface in addition, there are more ccg distributions in the endothelial cells, and the surface of the small vesicles and vesicles of the small vesicles only a very small number of ccg p