版權(quán)說明:本文檔由用戶提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請進行舉報或認(rèn)領(lǐng)
文檔簡介
ClinicalReproductiveEndocrinologyJackyChanClinicalReproductiveEndocrinClinicalReproductiveEndocrinologyReproductivehormonesAssaymethodsPhysiologyofreproductivehormonesinthefemaleClinicalaspectsofreproductiveendocrinologyGeneralcommentsClinicalReproductiveEndocrinReproductivehormonesDefinitionofhormonesChemicalclassesofreproductivehormonesHormonereceptorsInterconversionofsteroidsintargettissuesSynthesisandclearanceofhormonesReproductivehormonesDefinitioDefinitionofhormonessynthesizedandsecretedbyductlessendocrineglandsminutequantitiesdirectlyintothebloodvascularsystemClassicendocrineglands:pituitary,thyroid,parathyroid,adrenal,pancreas,ovary,testis,placenta,pinealglandDefinitionofhormonessynthesDefinitionofhormonesReproductionpituitary,pinealglands,gonads,placentaadrenalandthyroidglandsuterus,hypothalamusDefinitionofhormonesReproducReproductiveendocrineExtrahypothalamiccentresHypothalamiccentresAnteriorpituritaryGnRHOvaryUterusPinealMelatoninEstrogens/progesteroneFSH/LHEstrogens/progesteronePGF2αEstrogensInhibinGonadotropin-releasinghormoneGonadotropichormoneSexsteroidhormonesReproductiveendocrineExtrahyChemicalclassesofreproductivehormonesPeptideandproteinhormonesReleasinghormones,InhibitoryhormonesHypophysealhormonesNeurohypophysealhormonesPlacentalgonadotropinsSubunitsSteroidhormonesProstaglandinsChemicalclassesofreproductiThehormonessecretedfromtheanteriorpituitaryThehormonessecretedfromthePeptideandproteinhormonesReleasinghormones,(GnRH,TRH,CRH,PRH,GH-RH)PeptidehormonesproducedwithinthehypothalamusAdenohypophysisInhibitoryhormonesGrowthhormone(GIH)Prolactin(PIH)portalveinTSH
Insulin,glucagonPeptideandproteinhormonesReReleasinghormonesGonadotropin-releasinghormone(GnRH)pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2(MWofGnRH1,182)regulatethereleaseofFSHandLHReleasinghormonesGonadotropinhypothalamic-pituitary-ovarianaxishypothalamic-pituitary-ovarianLHsendsthesamemessageandresultsinincreasedtestosteroneproductionbythetestisduetoLHeffectontheLeydigcellsofthetestis.
LHandFSHarereleasedintothesystemiccirculation,travelingtothetargetreproductiveorgans(testesandovary)wheresexsteroidhormonesarecontrolled.LHsendsthesamemessageandReleasinghormonesThyrotropin-releasinghormone(TRH)pGlu-His-Pro-NH2(MWofTRH362)regulatethereleaseofTSH,GH,andprolactinPathwaysofthyroidhormonemetabolismReleasinghormonesThyrotropin-ReleasinghormonesCorticotropin-releasinghormone(CRH)CRHisa41-aminoacidpeptideregulatethereleaseofACTHReleasinghormonesCorticotropiPeptideandproteinhormonesHypophysealhormonesGlycoprotein,FSHandLH,prolactinMWofLH30,000(bovine,ovine,procine,equine)MWofFSH32,000(ovine,equine)MWofprolactin23,000(bovine,ovine,procine)FSH,LH,TSH,ACTH–producedinbasophiliccellsProlactin–localizedinacidophiliccellsPeptideandproteinhormonesHyProlactinLuteotrophichormone
(LTH):acorpusluteum-stimulatingeffectinmouse,rat,dogandcat198a.a,MW23,300anda15-minutehalf-lifeinsheepincreasinginlactation(mamotropin)stimulatingthepigeoncropglandtosecret“cropmilk”ProlactinPeptideandproteinhormonesNeurohypophysealhormones
Oxytocin,Vasopressin(synthesizedinhypothalamus)PosteriorpituitaryreleaseAxoplasmicfluidStimulationoftheNcellPeptideandproteinhormonesNePeptideandproteinhormonesPlacentalgonadotropinsPMSG(eCG,MW70,000),aproteinhormoneinserum,producedbyfetaltrophoblasticcellsofpregnantmaresThefunctionofendometrialcupsbeginsonday36until120daysofpregnancy.hCG(MW36,000),aproteinhormone,extractedfromtheurineofpregnantwomenfrom30-40daysofpregnancyglucocortocoid,estrogen,progestin,relaxin…PeptideandproteinhormonesPlEndometrialcupsEndometrialcupsdevelopfromcellsofthechorionicgirdle,circumferentiallyaroundtheconceptusatapointwherethemembranesoftheallantoisandyolksacmeet.
EndometrialcupsEndometrialcu270days1.TherelationshipofeCGandCLduringequinepregnancy2.eCGhasbothFSH-likeandLH-likeactivity,itprovidesthestimulusfortheformationofaccessoryCLsandmaintainaplateauofplasmaprogesteronefromabout50-140days.
270days1.TherelationshipofOneStephCGUrinePregnancyTest
hCGisalsousedtotreatwomenwithcertainovariandisorders.ClinicaluseofhCG
OneStephCGUrinePregnancyTAthletesusehCGtoincreasethebodynaturalproductionoftestosteronewhichisoftendepressedbylongtermsteroiduse.HCGtherapyhasbeenfoundtobeveryeffectiveinthepreventionoftesticularatrophyandtousethebodyownbiochemicalstimulatingmechanismstoincreaseplasmatestosteronelevelduringtraining.ClinicaluseofhCG
AthletesusehCGtoincreasetPeptideandproteinhormoneshCGLHhCG-subunitα:96a.asubunitβ:N-terminalspecificforhCGLH-subunitα:96a.asubunitβ:119a.aspecificforLHFSH-subunitα:96a.asubunitβ:specificforFSHTSH-subunitα:89a.ainhumansubunitβ:112a.ainhumanPeptideandproteinhormoneshCPeptideandproteinhormonesSubunits,
,
-subunit,uniqueforeachhormonewithinaspecies,determinesthebiologicalactivity.PeptideandproteinhormonesSuChemicalclassesofreproductivehormonesSteroidhormonesderivedfromtheprecursor,cholesterolMW<500Estrogens(estradiol)Androgens(testosterone)Progesterones(progesterone)ChemicalclassesofreproducticholesterolC27H46OC21H30O2
C19H28O2
C18H24O2
cholesterolC27H46OC21H30O2C1ChemicalclassesofreproductivehormonesProstaglandinsagroupof20-CunsaturatedfattyacidsMW:300-400localhormones,shortbiologicalhalf-lifewithalocalactionfoundinmammaliantissues,uterusandprostataChemicalclassesofreproductiBiosynthesisofPGfromarachidonicacidperoxidaseBiosynthesisofPGfromarachiSynthesisofprostaglandinaffectedbyestrogen
PhospholipidsArachidonicacidProstaglandinH2PGEPGFPGDInactivatedPhospholipaseA2Estrogen↑oxytocinreceptors↑Progesterone↓ProstaglandinH2synthetaseEstrogen↑Progesterone↓:1.proestrus→estrus2.priortoparturitionActivatedPhospholipaseA2SynthesisofprostaglandinaffPGE2stimulatesvasculardilation
bronchiodilation
gastrointestinalanduterinecontraction
inflammatoryresponsemimicshormonesthatactthroughcyclicAMPinhibitsplateletaggregationPGF2
stimulatesvascularconstriction
bronchioconstriction
smoothmusclecontraction,e.g.intestinaltract,uterus
PGI2inhibitsplateletaggregation(increasescAMP)
vascularconstriction
bronchioconstriction
gastrointestinalanduterinecontractionFunctionofprostaglandins
PGE2stimulatesvasculardilatiovascularuteroovarianpathwaysforPGF2αUterinebranchofOVOvarianbranchofOVvascularuteroovarianpathwaysvascularuteroovarianpathwaysforPGF2αSystemicuteroovarianpathway:Localuteroovarianpathway:Combinedsystemicandlocaluteroovarianpathway:horse,rabbitsheep,cattle,(guineapig,hamsters,swinerats)?vascularuteroovarianpathwaysClinicaluseofPGF2
endometritis/pyometraClinicaluseofPGF2endometriClinicaluseofPGF2
inductionofestrusClinicaluseofPGF2inductionOnsetofestrusOvulationprogesteroneCorpusluteum/NormalestruscycleOnsetofestrusOvulationShortenestruscyclewithPGF2
PGF2
injectionforinductionofestrusOnsetofestrusOvulationOnsetofestrusprogesteroneCorClinicaluseofPGF2
inductionofabortion/parturitionClinicaluseofPGF2inductionClinicaluseofPGF2
inductionofabortion/parturitionClinicaluseofPGF2inductionClinicaluseofPGF2
inductionofabortion/parturitionClinicaluseofPGF2inductionClinicaluseofPGF2
misallianceinductionofabortionendometritis/pyometraonlyinthecaseofopenpyometra!!ClinicaluseofPGF2misallianHormonereceptorsSteroidhormonesfatsoluble,abletoenterallcellslowconcentration(estrogen:10-150pmol/Linnon-pregnantfemale)Receptorsenablethetargetcells
-toconcentratethehormonewithinthecell-toelicitparticularcellularresponses-tocreateabiologicalresponseonspecificbindingHormonereceptorsSteroidhormoThesteroidhormonereceptorismadeupofthreedomains:amodulatingN-terminaldomain(transcription-activationdomain),aDNA-bindingdomainaC-terminalsteroid-bindingdomain.
ThesteroidhormonereceptoriHormonereceptorspassivediffusionTargetcellsreceptorsnon-protein-boundhormoneprotein-boundsteroidhormonesteroid-receptorcomplexRNAsynthesis↑proteinsynthesis↑TranslocationofcomplexintonucleusE2:mammaryglandP4:endometriumT:sertolicellHormonereceptorspassivediffuHormonereceptorsTargetcellsreceptorsactionsofFSHandLHingonadsProteinhormoneprotein-receptorcomplexcyclicAMPproteinkinaseactionsofcholesterolGproteinHormonereceptorsreceptorsacti牲畜生殖內(nèi)分泌學(xué)課件TissuesresponsivetoandrogensTissuesresponsivetoandrogenInterconversionofsteroidsintargettissuestestosterone5α-reductase5α-dihydrotesterone(5α-DHT)TargetcellsInterconversionofsteroidsinProgesteroneconversionintheplacentaCholesterolPregnenoloneProgesterone17α-HydroxyprogesteroneAndrostenedioneEstrone17α-HydroxylaseCortisolTestosteroneEstradiol17βPGF2α↑parturitionProgesteroneconversionintheSynthesisandclearanceofhormonesSynthesisandclearancearemainthefactors,whichinfluencethehormoneconcentrations.Theconcentrationofahormonereflectsitsrateofsynthesisorsecretion.metabolizedbyliverdetectionofthebodyfluids(plasma,urine,saliva,feces…)reproductivecondition,exwildanimalsSynthesisandclearanceofhorSynthesisandclearanceofhormonesunderthecontrolofgonadotropinsinpulsatilemode♂Thesecretionoftestosterone
changesinpulsatilerate.varyingfrom3.5–20nmol/Lwithinhours♀ThepulserateofE2andP4
remainsstable.SynthesisandclearanceofhorSynthesisandclearanceofhormonesPlacentagonadotropins(hCG,eCG)producedinhighconcentrationlongerhalf-lifethanthepituitarygonadotropinsFSH,LH≒10-30minuteshCG≒1.5dayseCG≒6daysequineLH>otherspeciesSynthesisandclearanceofhorSynthesisandclearanceofhormonesPGF2α15-keto-13,14-dihydro-PGF2αHalflifeprimaryPGF2α<20seconds15-keto-13,14-dihydro-PGF2α≒8minutes25mgPGF2α,IMincows>90%excretedintheurineandfeces(2:1)over48hoursSynthesisandclearanceofhorAssayMethodsQuantificationofspecifichormonesisnecessaryfortheendocrinologicalevents.Weight-gainchangechemicaldeterminationsforsteroidhormonesurineanalysisAssayMethodsQuantificationofImmunoassaytechniqueAgAbImmunoassaytechniqueAgAbAssayMethodsImmunoassayRadio-immunoassay(Bersonetal.,1959),Sen:10-9Enzymemultipledimmunoassaytechnique,Sen:10-6Fluoescineimmunoassay,Sen:
10-9EIA(Engvalletal.,1970s),Sen:10-12Chemiluminescenceimmunoassay,Sen10-15AssayMethodsImmunoassayRadioimmunoassayBecauseoftheeasewithwhichiodineatomscanbeintroducedintotyrosineresiduesinaprotein,theradioactiveisotopes125Ior131Iareoftenusedastheradioactiveantigen.RadioimmunoassaySandwichEIASandwichEIAcompetitiveEIAcompetitiveEIAsemiquantitativeELISAtestsemiquantitativeELISAtestThechemicalluminolisoxidisedbyH2O2,toproduceamoleculeinanexcitedstate.Whentheexcitedelectronsinthemoleculesfallbacktotheirgroundstates,lightisgivenout.LuminolExcitedState
GroundStateThechemicalluminolisoxidisAssayMethodsImmunoassayRadio-immunoassay(Bersonetal.,1959),Sen:10-9CompetitiveproteinbindingEIA(Engvalletal.,1970s),Sen:10-12Chemiluminescenceimmunoassay,Sen10-15DiagnosticaidsTherapy-monitoringAssayMethodsImmunoassayNorthernsemi-sphereSubtropicalTaiwanMonitoringtheseasonalestrusactivityofmareNorthernsemi-sphereSubtropicaPhysiologyofreproductivehormonesinthefemaleEstrouscycleControlofthecorpusluteumEarlypregnancyPregnancyandparturitionPhysiologyofreproductivehorEndocrinecontrolofcyclicalreproductiveactivityEndocrinecontrolofcyclicalThedurationofestrus,timeofovulationanddurationofCLfunctionindomesticanimalsThedurationofestrus,timeoInfluenceofthelengthofdaylightontheovaryfunctioninmaresPhotoreceptorsintheeyesPinealHypothalamusAnteriorpituitaryOvariesincreasingdaylightdecreasingdaylightneurotransmissionportalsystembloodcirculationMelatoninMelatoninGnRHGnRHGonadotropineGonadotropineInfluenceofthelengthofdayestrousbehaviorofmaresestrousbehaviorofmaresLargeamountsofequineLHarereleasedduringan8-to9-dayperiodwithovulationoccurringonthe3rdto5thday.
Controlofthecorpusluteum
8-9daysLargeamountsofequineLHare牲畜生殖內(nèi)分泌學(xué)課件thecainternahasLHreceptorandproducestestosterone.granulosacellhasFSHreceptorwhichcausescelltotaketestosteronefromthecaandconvertittoestradiol.dominantfolliclehasLHreceptorsongranulosawhichcanrespondtoLHtodriveprogesteroneproduction.
Eachgranulosacellhasapproximately1500FSHreceptors
P4+feedbackLH
thecainternahasLHreceptorOvariansteroidogenesisFSHcausesincreasedaromataseactivity,whichconvertstestosteronetoestradiolProductionRateofSexSteroidsinWomenatDifferentStagesoftheMenstrualCycle
DAILYPRODUCTIONRATESEXSTEROIDS*Early
Follicular
Preovulatory
MidlutealProgesterone(mg)142517Hydroxyprogesterone(mg)0.5
4
4
Dehydroepiandrosterone(mg)77
7
Androstenedione(mg)2.64.73.4Testosterone(mg)144
171
126
Estrone(mg)50
350
250
Estradiol(mg)36380250OvariansteroidogenesisFSHcaucumuluscellsproducehyaluronicacidandproteinstocausecumulusexpansionincreasedbloodflowtofollicle(vascularpermeability↑)ovulationcumuluscellsproducehyaluron牲畜生殖內(nèi)分泌學(xué)課件Triggersincreaseproteinandsteroidsynthesis,andcellulardifferentiationwithinfolliclePlasminogenisconvertedtoplasminbyplasminogenactivator(activecollagenase↑).Prostaglandinstimulatessmoothmuscletocontractandforcetherupturedfollicletoempty.
ovulationTriggersincreaseproteinand牲畜生殖內(nèi)分泌學(xué)課件ControlofthecorpusluteumCLisresponsiblefortheestrouscyclicity.UteruscontrolsthelifespanoftheCLinthecow,ewe,sowandmare.EstrogensinitiateuterustosynthesizesPGF2α.RegressionofCLisaccomplishedwithin48hrs.ControlofthecorpusluteumCLControlofthecorpusluteumEstrogensendometrialoxytocinreceptorsinitiatethereleaseofPGF2α,whichcausesthereactionoflutealregression.Inruminants,releasingofPGF2αisinducedbyoxytocinsecretedbytheCL.Indogandcat,PGF2α
CL?ControlofthecorpusluteumEsControlofthecorpusluteumLHstimulatesthesecretionofprogesteronefromsmalllutealcellswhilePGF2αinhibitssecretionofprogesteronefromlargelutealcellsandcausesdegenerationofthesecells.ControlofthecorpusluteumLHEarlypregnancyMaternalrecognitionmodificationofPGF2α
bytheluteotropicproductsfromtheconceptusanduterusTrophoblastproteins(cow,ewe,doe)Estrogens(sow,mare)intrauterinemobilityoftheembryo(mare)?(bitch,queen)antiluteolyticactionorluteotrophiceffecttoextendthelutealactivityEarlypregnancyMaternalrecognEarlypregnancyTrophoblastproteins(cow,ewe,doe)
oTP-1secretedbetween10and21-24daysbTp-1secretedbetween16-19dayscTP-1secretedonDay17tobinduterineoxytocinreceptorstoblocktheuterinePGF2αsecretionEarlypregnancyTrophoblastproTrophoblastproteinsOvinetrophoblastprotein(oTP-1,IFN-τ)MW18,000,producedonDay10inhibitingtheuterineoxytocinreceptorsinhibitingthesynthesisofPGF2αPGF2↓,PGE2↑TrophoblastproteinsOvinetropTrophoblastproteinoxytocinreceptorTrophoblastproteinoxytocinreEarlypregnancyConceptus-derivedestrogens(sow,mare)estroneandestradiol17-β
convertedfromprogesteroneonDay11,Day14-18rapidenlongationoftheblastocystreleasingofcalcium,specificproteinsalteringanendocrineofPGtoanexocrinePGmetabolizedtoinactivePGFMPGF2PGE2EarlypregnancyConceptus-derivThesecretionofestrogensbytheblastocyststimulatesthereleaseofcalcium,specificpolypeptidesandproteins.alteringthePGF2fromtheendocrinetoanexocrineintrauterinePGF2metabolisedtoinactivePGFMbyfetalmembranesThesecretionofestrogensbyThereisextensivemobilityoftheequineconceptuswithinthehornsanduterinebodybeforefixationoccursbetweendays16and18.??EndometrialProstaglandinSynthesisInhibitor,(EPSI)fromendometriumThereisextensivemobilityofTimeofmaternalrecognitionofpregnancy
DayofDayofdefiniterecognitionattachmentMare14-1636-38Cow16-1718-22Sow1218Ewe12-1316Goat17TimeofmaternalrecognitionoCorpusluteum&PregnancyP4fromCL:cow,sow,goat,dogandcatP4fromCL+fetoplacentalunit:mare,sheep
Corpusluteum&PregnancyP4frPregnancy1.Thesheepplacentaproducesenoughprogesteronebyday60-70.2.Lutealprogesteroneisrequiredthroughoutgestationincattleandgoatsbecausetheirplacentasecretemuchsmallerquantitiesofprogesterone.Pregnancy1.Thesheepplacenta牲畜生殖內(nèi)分泌學(xué)課件Fetal-PlacentalUnitDihydroepiandrosteroneFetal-PlacentalUnitDihydroepiParturitionwithin12hoursParturitionProgesterone
Parturitionwithin12hoursParParturitionParturitionFetalhypothalamusFetalpituitaryACTHFetaladrenalAdrenalcorticosteroidsFeto-placentalestrogensCotyledons/myometriumReleaseofPGF2
MyometrialcontractionsPosteriorpituitaryOxytocinFetalmaturationDecreaseinplacentalP4LuteolysisoftheCLofpregnancyDecreaseinserumP4IncreasedmyometrialexcitabilityAdrenalineRelaxationandsofteningofcervixpelvicligaments,perineumRelaxin?ExpulsionoffetusStimulatesvaginaandcervixAbdominalstrainingCortisol
ParturitionFetalhypothalamusFetalpituitParturitionProstaglandinsaresolubleinfatandwater,ProstaglandinspassfromcelltocellActions:smoothmusclecontractionluteolysissofteningofcervicalcollagendevelopinggapjunctionsmovementofCa2+betweenthemyofibrilsPGF2α,PGE↑,PGI2↓ParturitionProstaglandinsareSmoothmusclecellsSmoothmusclecellsmolecules(<1000daltons)thatcanpassincludecalciumions,cAMP,ATP,glutathione,andlargemoleculeslikeaminoacidsandsugarsetc.
molecules(<1000daltons)thaSmoothmusclecontractionMuscleprotein:myosinandactinCalmodulinactivatesMLCkinaseCalcium-CalmodulinMLCkinaseinactivemyosinlightchaincontractionsactomyosincomplex
(M*-ATP)+A<---->(M*-ADP-A)+Pi(M*-ADP-A)<----->(M-A)+ADP
SmoothmusclecontractionMusclSmoothmusclecontraction1.Calciumionscomefromoutsideofthecell.2.Calciumionsbindtocalmodulin-MLCK
complexonmyosin.3.TheenzymecomplexbreaksupATPintoADPandtransfersthePidirectlytomyosin.4.ThisPitransferactivatesmyosin.5.Myosinformscross-bridgeswithactin.6.Whencalciumispumpedoutofthecell,thePigetsremovedfrommyosinbyphosphatase.7.Themyosinbecomesinactive,andthemusclerelaxes.Smoothmusclecontraction1.CThetopviewshowsarelaxedsmoothmusclecell.
Notethefocaldensitiesandthenetworkofactinandmyosinfilaments.
Whencontracted,thefilamentsslidetogetherandpullthecelltoamoreroundedappearance.SmoothmusclecontractionThetopviewshowsarelaxedsCalmodulinisoneoftheprimaryreceptorsofintracellularcalcium.4Ca2+bindtocalmodulin.Ateachbindingsite,Cainteractswithoxygenatoms,mainlyglutamateandaspartaseside-chaincarboxylgroup.Calmodulinmediatesprocessessuchasinflammation,metabolism,apoptosis,musclecontraction,nervegrowth,immuneresponse….CalmodulinisoneoftheprimaParturitionActionsofestrogenscontractileproteinsynthesis↑receptorsofoxytocinandPGF2α
↑calmodulinsynthesis↑MLCkinaseactivity↑gapjunctions↑ParturitionActionsofestrogenParturitionActionsofprogesteronegapjunctions↓receptorsofoxytocinandPGF2α↓synthesisofoxytocinandPGF2α↓calmodulinbinding↑ParturitionActionsofprogesteParturitionActionsofoxytocindependentonprogesterone↓,estrogens↑
stimulationofreceptorsintheanteriorvaginaand
cervixoxytocinreleaseincreasingPGreleaseincreasingCa2+releaseParturitionActionsofoxytocinClinicalaspectsofreproductiveendocrinologySteroidhormoneconcentrationsarelowerinanimalsthaninhuman.(estrogens<1/10)Progesteroneanalysisgivethemostusefulinformationonthereproductivestatus.Blood,milk,urine,salivaandfecesaresourcesforendocrineinformation.ClinicalaspectsofreproductiCow:P4forearlydiagnosisofpregnancyNegativeforecast(98%)isbetterthanpositiveforecast(67-90%).P4forpostpartumovarianactivityEstronesulfatefordiagnosisofpregnancyfromDay120,96%accuracyrateBovinepregnancyspecificproteinBforearlydiagnosisofpregnancyonDay30ClinicalaspectsofreproductiveendocrinologyCow:ClinicalaspectsofreprodP4forpostpartumovarianactivityorearlydiagnosisofpregnancySamplingtimeP4forpostpartumovarianactiFollicularcystOvariancystP4forpostpartumovarianactivityFollicularcystOvariancystP4P4forpostpartumovarianactiv
溫馨提示
- 1. 本站所有資源如無特殊說明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請下載最新的WinRAR軟件解壓。
- 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
- 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁內(nèi)容里面會有圖紙預(yù)覽,若沒有圖紙預(yù)覽就沒有圖紙。
- 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
- 5. 人人文庫網(wǎng)僅提供信息存儲空間,僅對用戶上傳內(nèi)容的表現(xiàn)方式做保護處理,對用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對任何下載內(nèi)容負(fù)責(zé)。
- 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容,請與我們聯(lián)系,我們立即糾正。
- 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時也不承擔(dān)用戶因使用這些下載資源對自己和他人造成任何形式的傷害或損失。
最新文檔
- 2023年畢節(jié)市七星關(guān)區(qū)紀(jì)委區(qū)監(jiān)委所屬事業(yè)單位考調(diào)筆試真題
- 2024年福建客運資格證應(yīng)用能力試題
- 2024年江西客運從業(yè)資格證考題
- 2024年吉林客運從業(yè)資格證考試題庫模擬考試
- 2024年濰坊道路旅客運輸考卷
- 2024年邢臺道路旅客運輸資格證考試
- 2024年遼陽客運資格證考試題庫下載
- 2024年南京客運從業(yè)資格考試題
- 旅游路線規(guī)劃行業(yè)競爭格局與投資戰(zhàn)略研究咨詢報告
- 警衛(wèi)服務(wù)行業(yè)分析及未來五至十年行業(yè)發(fā)展報告
- 2024年河南省科協(xié)直屬事業(yè)單位河南省科技館招聘20人歷年(高頻重點提升專題訓(xùn)練)共500題附帶答案詳解
- HY/T 0401-2024走航式溫鹽深剖面測量儀海上試驗方法
- 2024-2030年中國家用空調(diào)行業(yè)營銷動態(tài)與競爭趨勢預(yù)測研究報告
- 班組激勵承包
- 信號工模擬試題+答案
- 2024軌道交通絕緣配合第1部分:基本要求電工電子設(shè)備的電氣間隙和爬電距離
- DL-T5161.10-2018電氣裝置安裝工程質(zhì)量檢驗及評定規(guī)程第10部分:66kV及以下架空電力線路施工質(zhì)量檢驗
- DB11-T 1764.21-2022 用水定額 第21部分:屠宰及肉制品加工
- 醫(yī)療廢物管理臺賬模板
- 幼兒園一等獎公開課:小班繪本閱讀《小藍和小黃》動畫版課件
- 代建工程作業(yè)指南
評論
0/150
提交評論