版權(quán)說明:本文檔由用戶提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請進行舉報或認領(lǐng)
文檔簡介
第第頁20222分子生物學試題A卷號:〔A〕〔2022年12月〕絕密
湖北師范學院一般本科期末考試試卷
分子生物學
考試范圍考試形式
閉卷
全部章節(jié)課程類別
命題人
必修學期
王友如20222
系部生命科學學院專業(yè)生物技術(shù)
一、名詞說明〔翻譯并簡約說明,每題2分,共16分〕
1.tRNAi2.RNAi3.intein4.intron5.codingregion;6.codingstrand;7.operon8.operator;
二、單項選擇題(選擇正確答案,每題1.5分,共24分)
9.WhichenzymedoesnotplayaroleinDNAreplication?()
(A)DNApolymerase(B)Helicase
(C)Single-strandedbindingprotein(D)Guanyltransferase10.Splicingistheprocessthatdoeswhichofthefollowing?()
(A)Removeintronsandconservee*ons(B)Removee*onsandconserveintrons
(C)RemovemutatedregionsofprimarytranscriptRNA
(D)AddmultipleadenosinebasestotheendofaprimaryRNAtranscript11.The5'cappingprocesscreateswhattypeoflinkage?()
(A)5'-3'(B)3'-5'(C)5'-5'(D)3'-3'
12.Codonsthatcodeforthesameaminoacidarecalled:()
(A)Synonyms(B)Similar(C)Degenerates(D)Complements
13.Whichvectorwouldyouchoosetoe*pressaforeigngeneinaplant?D
Aabaculovirusvector.Baretroviralvector.CaYepvector.DaT-DNAvector.14.Whatsignalstheendoftranscription?()
(A)Stopcodon(B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsout
15.Whatisthenameofthemoleculethatisaddedtothe5'endofeukaryoticRNAtranscripts?()
(A)ATP(B)SnRNP(C)GTP(D)Phosphate
16.Accordingtothewobblehypothesis,aUinthe5'positionoftheanitcodoncanpairwith:()
(A)UorC(B)AorG(C)A,C,orG(D)OnlyA
17.Whichtwoofthefollowingstatementsabouttranscriptionfactorsaretrue?D
Atheheli*-turn-heli*domainisatranscriptionalactivationdomain.Bdimerizationoftranscriptionfactorsoccursthroughthebasicdomain.CleucinezippersbindtoDNA.
DitisoftenpossibletogetfunctionaltranscriptionfactorswhenDNAbindingdomainsandactivationdomainsfromseparatetranscriptionfactorsarefusedtogether.
18.Whichofthefollowingisnotcharacteristicofprokaryoticterminators?()
(A)Rho-independentterminators(B)Rho-dependentterminators(C)TheTATAbo*(D)Hairpinloops
19.WhatistheenzymeresponsibleforstitchingtogetherOkazakifragments?()
(A)Helicase(B)Single-strandedbindingprotein(C)Primase(D)Ligase
20.DenaturationofdoublestrandedDNAinvolves.Apreakageintoshortdouble-strandedfragments.Bseparationintosinglestrands.ChydrolysisoftheDNAbackbone.
Dcleavageofthebasesfromthesugar-phosphatebackbone.
21.Inprokaryotes,thelaggingstrandprimersareremovedby...A3'to5'e*onuclease.BDNAligase.
CDNApolymeraseI.DDNApolymeraseIII.
22.Prokaryoticplasmidscanreplicateinyeastcellsiftheycontainaclonedyeast..
AORC.BCDK.CARS.DRNA.
23.Pernucleotideincorporated,thespontaneousmutationfrequencyinE.coliis...
A1in106.B1in108.
C1in109.D1in1010.
24.Whichtwoofthefollowingstatementsaboutattenuationatthetrpoperonaretrue?Aattenuationisrho-dependent.
Bdeletionoftheattenuatorsequenceresultsinanincreaseinbothbasalandactivatedlevelsoftranscriptionfromthetrppromoter.
Ctheattenuatorliesupstreamofthetrpoperatorsequence.
Dattenuationdoesnotrequiretightcouplingbetweentranscriptionandtranslation.
Epausingofaribosomeattwotryptophancodonsintheleaderpeptidewhentryptophanisinshortsupplycausesattenuation.
25.Whichoneofthefollowingstatementsaboutcatabolite-regulatedoperonsisfalse?AcAMPreceptorprotein(CRP)andcataboliteactivatorprotein(CAP)aredifferentnamesforthesameprotein.
BwhenglucoseispresentinthecellcAMPlevelsfall.CCRPbindstocAMPandasaresultactivatestranscription.DCRPbindstoDNAintheabsenceofcAMP.
ECRPcanbendDNA,resultinginactivationoftranscription.
三、填空題(每空1分,共10分)
26.Thesimplestcloningvectoratleastcontainsandorielements.27.ThediagrambelowshowstwoRNAmolecules.MoleculeIisprocessed
togivemoleculeII.Answerthefollowingquestionsaboutthesetwomolecules:Wheredoesthisconversionhappen?____________________________WhattypeofRNAmoleculeismoleculeI?_________________________WhattypeofRNAmoleculeismoleculeII?_________________________Nametheregions/structuresindicatedbythearrows:A=_________________B=_______________
C=_________________D=______________E=________
四、簡答題〔回答要點,每題4分,共五小題,共20分〕
28WhenyoustudytheDNAcomponentsofphageΦΧ174,yougettheresultsasfollows:A,25%;T,33%;G,24%;C,18%.Question(1):Doesyourresultsabidebybasepairingrules?Question(2):Howdoyoue*plainyourresults?
29.DNAligaseplaysimportantroleintheprocessofDNAreplication,butligasedoesnotfunctionintheprocessofRNAsynthesis.Couldyoue*plainit?30DoyouthinkthatrRNAandtRNAaremorestablethanmRNA?Why?31.HowdoesacodingregionDNAsequencecodedifferenttypesofprotein?32.Couldyoulist4kindsofRNAthathavetheabilitytocatalyticactivity?
五、問答題〔每題10分,共20分〕。
33.PleasetellusthetypesofRNAthatyouknow,itwouldbebetterthatyoue*plaintheirfunctioninlivingorganisms.(10points)
34.Takethecaseofarabinoseoperon,Couldyoue*plainwhatcis-actingelementandtrans-actingfactorare,howdotheyinteractwitheachothertoregulatethisoperon?(10points)
六、分析題〔每題10分,共10分〕35.AscientistgotagalpromotermutantE.coli.whichcannotusegalactose(半乳糖)inthemedium,ifhemutatedthegenecya(geneofcAMP環(huán)化酶)andcrp(cAMPAcceptorProteingene)weremutatedintocya-andcrp-respectively,thesecondmutatedE.coli.restoreditsabilitytousegalactose.Couldyoue*plaintheresultsbyusingyourmolecularbiologicalknowledge?
卷號:〔A〕〔2022年12月〕絕密
湖北師范學院一般本科期末考試試卷
分子生物學
考試范圍考試形式
閉卷
全部章節(jié)課程類別
命題人
必修學期
王友如20222
系部生命科學學院專業(yè)生物技術(shù)
一、名詞說明〔翻譯并簡約說明,每題2分,共16分〕
1.tRNAi2.RNAi3.intein4.intron5.codingregion;6.codingstrand;7.operon8.operator;
二、單項選擇題(選擇正確答案,每題1.5分,共24分)
9.WhichenzymedoesnotplayaroleinDNAreplication?()
(A)DNApolymerase(B)Helicase
(C)Single-strandedbindingprotein(D)Guanyltransferase10.Splicingistheprocessthatdoeswhichofthefollowing?()
(A)Removeintronsandconservee*ons(B)Removee*onsandconserveintrons
(C)RemovemutatedregionsofprimarytranscriptRNA
(D)AddmultipleadenosinebasestotheendofaprimaryRNAtranscript11.The5'cappingprocesscreateswhattypeoflinkage?()
(A)5'-3'(B)3'-5'(C)5'-5'(D)3'-3'
12.Codonsthatcodeforthesameaminoacidarecalled:()
(A)Synonyms(B)Similar(C)Degenerates(D)Complements
13.Whichvectorwouldyouchoosetoe*pressaforeigngeneinaplant?D
Aabaculovirusvector.Baretroviralvector.CaYepvector.DaT-DNAvector.14.Whatsignalstheendoftranscription?()
(A)Stopcodon(B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsout
15.Whatisthenameofthemoleculethatisaddedtothe5'endofeukaryoticRNAtranscripts?()
(A)ATP(B)SnRNP(C)GTP(D)Phosphate
16.Accordingtothewobblehypothesis,aUinthe5'positionoftheanitcodoncanpairwith:()
(A)UorC(B)AorG(C)A,C,orG(D)OnlyA
17.Whichtwoofthefollowingstatementsabouttranscriptionfactorsaretrue?D
Atheheli*-turn-heli*domainisatranscriptionalactivationdomain.Bdimerizationoftranscriptionfactorsoccursthroughthebasicdomain.CleucinezippersbindtoDNA.
DitisoftenpossibletogetfunctionaltranscriptionfactorswhenDNAbindingdomainsandactivationdomainsfromseparatetranscriptionfactorsarefusedtogether.
18.Whichofthefollowingisnotcharacteristicofprokaryoticterminators?()
(A)Rho-independentterminators(B)Rho-dependentterminators(C)TheTATAbo*(D)Hairpinloops
19.WhatistheenzymeresponsibleforstitchingtogetherOkazakifragments?()
(A)Helicase(B)Single-strandedbindingprotein(C)Primase(D)Ligase
20.DenaturationofdoublestrandedDNAinvolves.Apreakageintoshortdouble-strandedfragments.Bseparationintosinglestrands.ChydrolysisoftheDNAbackbone.
Dcleavageofthebasesfromthesugar-phosphatebackbone.
21.Inprokaryotes,thelaggingstrandprimersareremovedby...A3'to5'e*onuclease.BDNAligase.
CDNApolymeraseI.DDNApolymeraseIII.
22.Prokaryoticplasmidscanreplicateinyeastcellsiftheycontainaclonedyeast..
AORC.BCDK.CARS.DRNA.
23.Pernucleotideincorporated,thespontaneousmutationfrequencyinE.coliis...
A1in106.B1in108.
C1in109.D1in1010.
24.Whichtwoofthefollowingstatementsaboutattenuationatthetrpoperonaretrue?Aattenuationisrho-dependent.
Bdeletionoftheattenuatorsequenceresultsinanincreaseinbothbasalandactivatedlevelsoftranscriptionfromthetrppromoter.
Ctheattenuatorliesupstreamofthetrpoperatorsequence.
Dattenuationdoesnotrequiretightcouplingbetweentranscription
溫馨提示
- 1. 本站所有資源如無特殊說明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請下載最新的WinRAR軟件解壓。
- 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
- 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁內(nèi)容里面會有圖紙預(yù)覽,若沒有圖紙預(yù)覽就沒有圖紙。
- 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
- 5. 人人文庫網(wǎng)僅提供信息存儲空間,僅對用戶上傳內(nèi)容的表現(xiàn)方式做保護處理,對用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對任何下載內(nèi)容負責。
- 6. 下載文件中如有侵權(quán)或不適當內(nèi)容,請與我們聯(lián)系,我們立即糾正。
- 7. 本站不保證下載資源的準確性、安全性和完整性, 同時也不承擔用戶因使用這些下載資源對自己和他人造成任何形式的傷害或損失。
最新文檔
- 3-2《哦香雪》公開課一等獎創(chuàng)新教學設(shè)計 統(tǒng)編版高中語文必修上冊
- 統(tǒng)編版六年級語文上冊第三單元整體核心素養(yǎng)公開課一等獎創(chuàng)新教學設(shè)計
- 2024合同法中違約金的有關(guān)規(guī)定
- 2024商業(yè)貸款借款合同范本
- 2024后合同義務(wù)及其法律特征
- 2024單方勞動合同解除
- 2024LOGO廣告制作安裝合同
- 2024商務(wù)辦公樓租賃合同
- 二年級故事課件
- 基于學生發(fā)展指導(dǎo)的“語文+”學科融合課程模式建設(shè)
- 應(yīng)急第一響應(yīng)人理論考試試卷(含答案)
- MOOC 跨文化交際通識通論-揚州大學 中國大學慕課答案
- 六西格瑪-DMAIC-報告
- 部編九年級語文上冊第二單元大單元教學設(shè)計
- 檢驗科技術(shù)人員基本技能考核表
- 通用英語答題卡word模板
- 26個英文字母卡片
- 肩難產(chǎn)的處理ppt課件
- 廣播電視新聞評論第四講
- 合理化建議模板
- 英語科技論文PPT課件
評論
0/150
提交評論