常用限制性?xún)?nèi)切酶酶切位點(diǎn)

1、Aatll 識(shí)別位點(diǎn)5；. G ACGT；h . .33CJGC AG, , .5JAcc65l 識(shí)別位點(diǎn)5g+mK AC 3，3. . CA KMJG. . 5JAccl識(shí)別位點(diǎn)煮GlK AC3，3J. . CA KMJG. . 5JAcil識(shí)別位點(diǎn)5 (ft GC 3計(jì)GG空莎Acll識(shí)別位點(diǎn)5 A A；GTT .33 . T T G CA A. 5Acul 識(shí)別位點(diǎn)5d. CTG A AG(N)< . . 3J3d. GACT TC(N)1<a. . . 5JAfel識(shí)別位點(diǎn)5. . AGCCT.,3P3. . TCGJCGA. 5JAflll 識(shí)別位點(diǎn)5cTtT A AG*

2、33：G A A T T&” 5"Afllll識(shí)別位點(diǎn)5： . £CR¥(31Y&TGYRqA.5*Agel識(shí)別位點(diǎn)5P. . /fCCGGT ,.3j3：TGGCqA .莎Ahdl 識(shí)別位點(diǎn)5P. G ACNNf/nNGTC . . 3P3- CTGNINNNCAG.5Alel識(shí)別位點(diǎn)5； . .CACNiTnnGTG 3'3. .GTGNINNCAC. . hAlul識(shí)別位點(diǎn)5P. . A(jfcT.3P3TQSAhAlwl 識(shí)別位點(diǎn)5 r,GGATC(N)73"3 CCTAG(N)y £AlwNl 識(shí)別位點(diǎn)5： .

3、 CAGNNNtCTG. . 3F3； . GTCNNNG AC. .5*Apal識(shí)別位點(diǎn)5 ., GGGCtft. .3P 3., CCCGGG .ApaLl 識(shí)別位點(diǎn)5. (TGCAC. ,3J3 . CACGTjGApeKl 識(shí)別位點(diǎn)5P. . . tCWGC., .3r3. CGWCjp., .5Apol 識(shí)別位點(diǎn)5； ,. FLATTY, ,3J3 , _ Y TTA AJl. T .5"Ascl識(shí)別位點(diǎn)5： ., GtfCGCGCC. .33 CCGCGCJjG, .5JAsel識(shí)別位點(diǎn)5 . B 4 A T't A A T t. , 3J3 . . T A A

4、TJ A . 5JAsiSl 識(shí)別位點(diǎn)5. .GCGAl1SGCr. 3r3. .CGCJAGCG . .5Aval 識(shí)別位點(diǎn)可cTVcgrg33：.GRGCYJ>.hAvall 識(shí)別位點(diǎn)5：dfewCC,.,3r3；., CCWG. 5JAvrll 識(shí)別位點(diǎn)5. . CtCT AGG .3GGATCf5Bael識(shí)別位點(diǎn)5：気N)AC(NLGTA¥C(N)3 3kli(N)TG(N),CATRG(N).5BamHl 識(shí)別位點(diǎn)呂," gEatCC33C C T A Gp -. 5"Banl識(shí)別位點(diǎn)呂GYRCC 33 CCRYQp .5JBanll 識(shí)別位點(diǎn)5：

5、. GRGC Yt 3", CaY C G R G . ,Bbsl識(shí)別位點(diǎn)5. .,GAAGAC (N)/ . .3r3J. . 0 T T C T G 減丄S'BbvCI 識(shí)別位點(diǎn)5 C(TtCAGC.”.3*3 GGAGTaCG . ,SJBbvl 識(shí)別位點(diǎn)5 GCAGCHN；3*計(jì)CQTCG(N)iar .5Bccl識(shí)別位點(diǎn)5P. . CCATC(N)/,. 33 , .GGTAG(N). -5rkBceAl 識(shí)別位點(diǎn)5. . ACGGCtNjJ. ,33： . TGCCG(N)f5*Bcgl識(shí)別位點(diǎn)5 . T4N)CGA(NK TGC(N)J . .3J3；.(N)G

6、CT(N)；ACGN).b5BciVl 識(shí)別位點(diǎn)5. r,GT ATCC (N)J .3F3. .C AT AGG (Nt .5PBcll識(shí)別位點(diǎn)5. . T'feATC A,- 3J3：ACT AGJ., 5JBfal識(shí)別位點(diǎn)5：. (?T AG . .3r苗GA.ChBfuAl 識(shí)別位點(diǎn)5；. . ACCTGC(N)J 33. .TGG ACG(N), .5Bgll識(shí)別位點(diǎn)5；. GCCNNNNJGGC., 3P3 . . CGGrhlNNNCCG. , 5*Bglll 識(shí)別位點(diǎn)莎A T G T33：”. TCTAtA. 5rBlpl識(shí)別位點(diǎn)5G(7TN AGO., -3J3；.,

7、 CGAN T；CG , .5Bme1580l識(shí)別位點(diǎn)5 . . GKGCWit 才3 . . QMCGKG ,5JBmgBl識(shí)別位點(diǎn)hC AC令TC孑3 . . .GTGJ： AG. . 5*Bmrl 識(shí)別位點(diǎn)5. . ACTGGG(N) T r.3r3. . TGACCC(N)4r.5-Bmtl 識(shí)別位點(diǎn)hGCTA(?b 33, C/3 ATCG 5Bpml 識(shí)別位點(diǎn)5 . CTGGAG(N)1pt 3r3. . GACCTC(N)14j.5rBpulOI 識(shí)別位點(diǎn)5CCTt N AGC *33., GGANCG . 5BpuEl 識(shí)別位點(diǎn)5；. CT TG AG(N)rT ,.3'

8、;3. GAACTC(N)a.S'BsaAl 識(shí)別位點(diǎn)5； . YAC'GTR . .3BsaBl 識(shí)別位點(diǎn)5： .GATNNNATC , .33：, ,CTANN/1NTAG,. . 5BsaHl 識(shí)別位點(diǎn)5P. . .GFitG YC . . ,33. .C YGCRG. .5JBsal識(shí)別位點(diǎn)5. . GGTCTC (N)/. .33. . C C A G A G (N). . . 5'BsaJl 識(shí)別位點(diǎn)5 c'b NNGG. 3J3； GGNNC/3,. 5BsaWl識(shí)別位點(diǎn)5 . ., w'bCGGW, 3J3WGGC即hBsaXl 識(shí)別位點(diǎn)

9、5 . NjACfNCTCCtN),； . 3-3r .(N)TG(N)r GAGG(N). .，& 丄pABseRl 識(shí)別位點(diǎn)5GAG GAG的3"3. . CTCCTCN)-a.5pBseYl 識(shí)別位點(diǎn)5, CV： CAGC.,. 3JCTGGGTQ3可Bsgl識(shí)別位點(diǎn)5. GTGCAGN), T . 33 . . CACGTCN)ltk . . 5'BsiEI識(shí)別位點(diǎn)5, CGRY'faG, 3P3 . .GCAyRGC. ,5BsiHKAI識(shí)別位點(diǎn)h - GWGCW七J3 _WCGWG. .5BsiWI 識(shí)別位點(diǎn)5 . ., C'fe T A

10、C G, . . 33.GC ATGJS. 5"Bsll識(shí)別位點(diǎn)5 . CCNNNN IINGG , 3*3；r. GGNNNNNNCC. 55；. TtC ATGA . 3F31A G T A CJ翼BspMI 識(shí)別位點(diǎn)5. . ACCTGC (N)/. T. 33. . TGG ACG (N)az.5BspQI 識(shí)別位點(diǎn)5 * GCTCTTC(I(t .33. .CGAGAAG(NU.5*BsrBI 識(shí)別位點(diǎn)5.CCdtTC,.3r3 GGCpAG. . .5BsrDI 識(shí)別位點(diǎn)5 . GC AATGNN, .3r3 . . . CGTTAqNN. , .5BsrFI 識(shí)別位點(diǎn)B

11、smAI 識(shí)別位點(diǎn)5r GTCTC(N)7 .3ri3. . C AG AG(N).5BsmBI 識(shí)別位點(diǎn)5. r,CGTCTC (N)J .3F3. . GC AGAG (N)s .5BsmFI 識(shí)別位點(diǎn)覚GGGAC(N)(DT . 3*3 CCCTG(N)11a. . .5JBsmI 識(shí)別位點(diǎn)5 . . . G A A T G C NT 丁3. . CT T ACSN .6JBsoBI 識(shí)別位點(diǎn)5P. .CVCG AG . 3P3 GRGC.G SBsp1286l識(shí)別位點(diǎn)5.GDGC 辰孑3qHCGDG 5BspCNI 識(shí)別位點(diǎn)5.iCTCAG(N)fT .33, .GAGTC (忡 h

12、. .5BspDI 識(shí)別位點(diǎn)5 . , A TTC G A T . f, 3*3 T AGCT A . .5BspEI 識(shí)別位點(diǎn)5“TCCGGA3 3 . . AGGCCJ , . 5BspHI識(shí)別位點(diǎn)5；. CGG Y . . 33；. YGGCQR 5“BsrGI 識(shí)別位點(diǎn)h Fb t a c a :r3：., AC AT GJ 5*BsrI識(shí)別位點(diǎn)5： . ACTGGNT . 3J3： , . T G A CC N . 5BssHII識(shí)別位點(diǎn)hG*CGCGC . .3P3：CGCOQp.護(hù)BssKI 識(shí)別位點(diǎn)5； . .'tCNGG .3:r 一. GGwcq 一 sBssSI

13、識(shí)別位點(diǎn)5(TaCGAG3j3".GTGCTf hBstAPI識(shí)別位點(diǎn)5； , GCANNNI7NTGC 3r 5：.CTCAG(N)¥ - CGTNNNNNACG.S 3 GAGTC (NL 5'梆圮stBI 識(shí)別位點(diǎn)5： k. T T'b G A A . . 33： T A A G CJ T r T 5JBstEII識(shí)別位點(diǎn)5 . G'feTN ACC . 3J3：,. CC AN TGJS. ,5JBstNI 識(shí)別位點(diǎn)5. cdVGG,. . 33：. GG咚C5BstUI識(shí)別位點(diǎn)5： . CG'faG,. .33 . . GCJ3C.

14、 .5BstXI 識(shí)別位點(diǎn)5 f .CCANNNNIsTnTGG . , 3j3 . f .GGTNaNNNNNACC. . . 5BstYI 識(shí)別位點(diǎn)5R先ATdS3, YCTAGR. .5JBstZ17l識(shí)別位點(diǎn)5GT fT AC卽3. CA TATG. .5-Bsu36l識(shí)別位點(diǎn)5.CC?TN AGG. . . 3r3-G G A N T& C.5"Btgl識(shí)別位點(diǎn)5；,. C?bHYGG,. .33；, GG YRCC , ,5JBtgZI 識(shí)別位點(diǎn)5GCGATG33；.CGCTAC(N),4j.5,BtsCI 識(shí)別位點(diǎn)5；. .GCAGTG N Nt 33： . C

15、 G T C A (N N . 5'BtsI識(shí)別位點(diǎn)5., ,GC AGTG N Nt. 3p3； . C G T C A <N N .Cac8I 識(shí)別位點(diǎn)GCNTNGC . 3J3：CGN/ICG密Clal識(shí)別位點(diǎn)ATfcGAT. . 3J3 T AGCJ At. 5CspCI識(shí)別位點(diǎn)5 :的 CAA (N>( GTGG (N).,33： +嚴(yán) JN) GTT (M), CACC(N1V，,5JCviAII 識(shí)別位點(diǎn)5Ct G:T3 r. G T AC . . SPCviKI-1識(shí)別位點(diǎn)5. .RGTC Y.3才¥C#GR5CviQI識(shí)別位點(diǎn)S'-. J

16、jTAC ,.3J3： 5棗5DdeI 識(shí)別位點(diǎn)5；-. CTNAG. .3J3； , G ANC.Dpnl 識(shí)別位點(diǎn)Ch,匚鳥(niǎo)；5G A T C33； . CT AG5Dpn II識(shí)別位點(diǎn)5； r tGATC. . 33：r, CT AG . 5JDral識(shí)別位點(diǎn)5；. T T TAA 33Rt A A A/TT . , 5JDralll 識(shí)別位點(diǎn)5； h CACNNN'bTG. . ,3“3GT砂Nhl SC5Drdl識(shí)別位點(diǎn)5 . , GACNNNNtNWGTC, ,33： . . CTGNNNNNNCAG., .5Eael識(shí)別位點(diǎn)5“gGCCR ;T3 . .RCCGG/ .

17、5JEael識(shí)別位點(diǎn)5, YfeGCCR, . 3J.RCCGG/ .Eagl識(shí)別位點(diǎn)5. . CSGCCG .3 . GCCGGf. .Earl識(shí)別位點(diǎn)5 CTCTTC (N)t .r3. . GAGA AG (N). .5PEcil識(shí)別位點(diǎn)5. . .GGCGGA(N)J . .33. .CCGCCT (N)Sa. ., 5JEcoNI 識(shí)別位點(diǎn)5P. . . CCTN(7nnnAGG 3"3；. . GG ANNNNNTCC . . , 5'EcoO109l識(shí)別位點(diǎn)5P. . R(jfeNCC Y :TS.YCCNG/SR . . .5EcoP15l識(shí)別位點(diǎn)5 r C

18、AGCAG(N)/ . P3r3 GTCGTC(Wy 5EcoRI識(shí)別位點(diǎn)5 . ., A T T C . 3 r 3；. . CTT AEcoRV 識(shí)別位點(diǎn)5.GATTC -,3*3： . CT AJ AG. .5JFatl識(shí)別位點(diǎn)5：先ATG33t GTAC . 5Faul識(shí)別位點(diǎn)5J CCCGC (NJ. .3'3；. GGGCG (N)#a. . ,5JFn u4HI識(shí)別位點(diǎn)昭GCNGC朝3. . CGN&G -SFokl識(shí)別位點(diǎn) 5l.GGATG(N)；1t 3j3. . CCTACtN), .5Fsel識(shí)別位點(diǎn)5 GGCCGG'tc, .33 . CC/3G

19、CCGG . . 5J Fspl識(shí)別位點(diǎn)5；,. TGCbC A . .3P3 ； ,. A C GJC G T . 5Haell識(shí)別位點(diǎn)5 RGCGCtY. . .33 LCGCGR 5，HaelllHgal識(shí)別位點(diǎn)5 . . .GACGCN/.3. . CTGCG<N1(W. . 5"Hhal識(shí)別位點(diǎn)5 GCdt 3" 宙中CG5，Hindi識(shí)別位點(diǎn)5： f GT YTR AC. .3r3 . CARAYTGfHin dlll識(shí)別位點(diǎn)5； . , A'A G C T T .3"3. . TTCGAA., .5JHi nfl 識(shí)別位點(diǎn)5 GklMT

20、C 33., CT NAJG., .5*HinPII 識(shí)別位點(diǎn) 亦dtGC:r3 . CGCp . . 5*Hpal識(shí)別位點(diǎn)5. . GTT> AC. .T3. , C A AJTG. _5JHpall識(shí)別位點(diǎn)5“cffeGG 33. GGCAC. 5JHphl 識(shí)別位點(diǎn)51 GGTGA (N)/.,.3, 3； . CC AC T (N)7i,., 5"Hpy188l識(shí)別位點(diǎn)5 T G “G A3 A0J CT £Hpy188lll識(shí)別位點(diǎn)5., TtfNNGA. .33., AGNNCT. , ,5jHpy99l 識(shí)別位點(diǎn)5； . CGWCGT3： F iGCWG

21、C. ,5HpyAV 識(shí)別位點(diǎn)5.hCCTTC(N)J,3-3：. GGAAG(Nk .5*%HpyCH4III識(shí)別位點(diǎn)hA C NG T 3"3 .,. T GN C A .5HpyCH4IVHpyCH4V 識(shí)別位點(diǎn)5；., Tdt A., .33,. A(3T, . .5JKasl識(shí)別位點(diǎn)5. , GjCGCC . , 3P 3r.CCGCG,.5JKpnl 識(shí)別位點(diǎn)5., , GGT AC't. 3 3QCATGG 5，Mbol 識(shí)別位點(diǎn)5P . .3ATC .33P . CT AG. .5Mboll 識(shí)別位點(diǎn)5； ., GA AGA (N3*3； , C T T C

22、T (N)Ja，. .5*Mfel 識(shí)別位點(diǎn)5；. C'X ATTG. .33；T . GTT A Ajp . . 5JMlul 識(shí)別位點(diǎn)5. . /Tcgcgt .,，3r3 ： . T G C G CA . 5JMlyl識(shí)別位點(diǎn)5 GAGTC(N, 一 33. .CTCA G (N) . 5-Mmel 識(shí)別位點(diǎn)5； . .TCC RAC(N)mt .3' 3： . ,AG G YTG(NJia>. .5*Mn II 識(shí)別位點(diǎn)5 .CCC (N/ . 33GGAG立,5-Mscl識(shí)別位點(diǎn)5 , F TG(?fc：C A t,3P3"ACCjGT. + j5jM

23、sel識(shí)別位點(diǎn)5. . f*T A A. 3P3； T. A A TJ . . 5Msll識(shí)別位點(diǎn)5 CAYNNVinRYG. .,3J3 GTRNNNNYAC 5MspAII識(shí)別位點(diǎn)5P. . . CMCSfcKG, . .33GKYMC擴(kuò)Mspl識(shí)別位點(diǎn)5；f. dfcGG 33； . . GGqp 5Mwol 識(shí)別位點(diǎn)5 . . G C N N N N NVj N G C. .3_ CGNNNNN NNCG.,Nael識(shí)別位點(diǎn)5 . GCC3GC,. .3r3：GGG&GG-.hNarl 識(shí)別位點(diǎn)5 GGt GCC. .33CCGQGG 5“Ncil識(shí)別位點(diǎn)5 .c(7sgg,.

24、 ,3r 肚GGS&ChNcol識(shí)別位點(diǎn)5. ,CtCATGG,. .3*3 ,. G G T A CJ3 . 5JNdel識(shí)別位點(diǎn)5； ., C /Tt A T G ,. 33. . GT AAChNgoMIV 識(shí)別位點(diǎn)5 . _ (itCGGC,. 33CGGCCG. . 5Nhel 識(shí)別位點(diǎn)5 . T AGO . .,3"3 GGATqp hNlalll 識(shí)別位點(diǎn)亍CATCf3AGhNlalV 識(shí)別位點(diǎn)5,. GGt/NCC-.3 . CCNNGG. .5JNmeAlll識(shí)別位點(diǎn)5：. GCCGAGNG3 .CGGCTC(N)ia.5rNotl 識(shí)別位點(diǎn)5 GdtGCC

25、GC ;r3 CGCCGtyCG 5Nrul 識(shí)別位點(diǎn)5 . BT TCGGA 3J3 MCFCT hNsil識(shí)別位點(diǎn)5 .ATGCTt . .33. . IACGTA . ,5JNspl 識(shí)別位點(diǎn)5 . RCATGTY. . 3J3；.，XhGTACR.,.5rPacl識(shí)別位點(diǎn)5. .TTAAfrTAA.3-3：+ , A ATJ A ATTtJ + 5jPaeR7l識(shí)別位點(diǎn)5；. CG AG . ,3P . + G AGCTJC , . 5JPcil識(shí)別位點(diǎn)5：A%島 TGT.3*3* . . TGT A CA. .S,APflFI識(shí)別位點(diǎn)5； , .GAChiVlNGTC .擴(kuò)3 . .

26、 . C T G N NAN C A G . 5* PflMI 識(shí)別位點(diǎn)5； . C C A N N N wTl T G G. . 33；GGTNaNNNNACC., .5Phol識(shí)別位點(diǎn)5：GGfcC33：. CCjjG. 5JPlel識(shí)別位點(diǎn)5. GAGTC.3*3：. CTCAG (N),. hMiPmel 識(shí)別位點(diǎn)5： . GTTTAAC. . .3J3； .T C AAAJ T TG.t 5JPmll 識(shí)別位點(diǎn)5； , C ACTG. .33P . GTGJ： AC- .5HPpuMI 識(shí)別位點(diǎn)5 , h RdTj W c C Y ”，3“3；_ YCC WGJ3R ., 5JPsh

27、AI 識(shí)別位點(diǎn)5； ACNl7NNGTC . + .3J3 , . .CTGNNJNNC AG . .5'Psil識(shí)別位點(diǎn)5；., TTT A AX . A A T A T T . SHJLPspGl 識(shí)別位點(diǎn)5 1tC WGG 3“3； r,GG WCC ,.5PspOMI識(shí)別位點(diǎn)5；,. (fGGCCC.,. 33：CCCGGJ35PspXl 識(shí)別位點(diǎn)5 V(7tCGAGB . b3J3. 8GAGCCV. .5*Pstl識(shí)別位點(diǎn)5 CTGC 屆.33；. . GACGTC-. S-Pvul 識(shí)別位點(diǎn)5 CG ATEG 3"3；. GCJ AGC. . . 5JPvull

28、 識(shí)別位點(diǎn)5；CAtSfcTG富. . G T CJ3 A C . 5Rsal識(shí)別位點(diǎn)5GTC33. . CAJG. .5*Rsrll 識(shí)別位點(diǎn)5；, ,C(?fewCCG. .3'3：GGC W棗ChSacl識(shí)別位點(diǎn)5 GAGCT ,3J3；CJCGAG, . 5JSacll 識(shí)別位點(diǎn)熱CCGC七G 23 . GGjpGCC . 5JSall識(shí)別位點(diǎn)5 GTTCGAC,. 3J3CAGCTS hSapl識(shí)別位點(diǎn)5； .-GCTCTTCtN) ,3J3 . r,CGAGAAG(N)(j.TJ5JSau3AI識(shí)別位點(diǎn)5 . 7g A T c .,. 33：. CT AG.5JSau96l

29、 識(shí)別位點(diǎn)5. . GSNCC . . 33 CCNGa hSbfl識(shí)別位點(diǎn)s.cctgcaTgg .,y3. , GG/CGTCC , 5Seal識(shí)別位點(diǎn)5： . A G T鼻 C T .甞3,. TC AJGA . _5JScrFI識(shí)別位點(diǎn)5 . CCTnGG . . 33. GGNJSC , . 5JSexAl 識(shí)別位點(diǎn)5 , .rfbCWGGT., ,3r3 TGGWCqA. - SSfaNI 識(shí)別位點(diǎn)5. , GC ATC (N)/ ,. 3*3J.,. CGT AG (N)9a，. 5*Sfcl識(shí)別位點(diǎn)5：(?TR¥AG ;T3： . .GAYRTC. . ,5Sfil識(shí)別位點(diǎn)5GGCCNNNhTNGGCC .,33. ,CCGGN/tNNNCCGG .5'Sfol識(shí)別位點(diǎn)5；., GGCCC . 3J3：CCGfGG 5，SgrAI 識(shí)別位點(diǎn)5 C RC CG G ¥ G 3"3. ,.G YGGCCjtAC,5HSmal識(shí)別位點(diǎn)5；., CCCGG. . 3r3 GEGfCC 一 5"Sm