Vanillin-p-Vanillin-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEVanillinCat. No.: HY-N0098CAS No.: 121-33-5Synonyms: p-Vanillin; m-Methoxy-p-hydroxybenzaldehyde; p-Hydroxy-m-methoxybenzaldehyde分式: CHO分量: 152.15作靶點(diǎn): Endogenous Metabolite作通路: Metabolic Enzyme/Protease儲(chǔ)存式: 4C, stored under nitr

2、ogen* In solvent : -80C, 6 months; -20C, 1 month (stored undernitrogen)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (657.25 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 6.5725 mL 32.8623 mL 65.7246 mL5 mM 1.3145 mL 6.5725 mL 13.1449 mL10 mM 0.6572 mL 3.2862 mL 6.5725 m

3、L請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍福渲魄罢?qǐng)先配制澄清的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (16.43 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 9

4、0% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (16.43 mM); Clear solution1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE3. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (16.43 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Vanillin從草中提取的單個(gè)分，也 在，品和藥物中泛使的味。IC50 & Target Human Endogenous Metabolit

5、e體外研究 Vanillin recovers UVA-induced reduction of proliferation in a dose dependent manner. Vanillin has noapoptotic effects at the tested concentrations. In addition, the reduced expression levels of OCT4, NANOG,and SOX2 caused by UVA irradiation are allincreased by Vanillin treatment, suggesting th

6、at Vanillinattenuates the effects of UVA irradiation on hAMSCs. Based on a luciferase reporter assay, Vanillinincreases the reduced activity of HRE-luciferase reporter caused by UVA irradiation. In addition, treatmentwith Vanillin attenuates the reduced expression of HIF-1 caused by UVA irradiation.

7、 The results reveal thattreatment of hAMSCs with Vanillin results in significant decrease in the production of PGE2 and cAMP whencompare to UVA-irradiated controls 1.體內(nèi)研究 Following antidepressant treatment for 4 weeks, immobility time in the stress+Vanillin and stress+fluoxetinegroups is significant

8、ly decreased F(4,42)=34.73, P 2.PROTOCOLKinase Assay 1 hAMSCs are irradiated with the indicated doses of UVA and then incubated with the indicated concentrationsof Vanillin for three days under serum-free conditions. After the incubation, the concentrations of PGE2 orcAMP in the culture supernatant

9、are measured using ELISA kits. Culture supernatants are added into 96 wellplates. Alkaline phosphatase conjugated PGE2 or cAMP and antibodies to PGE2 or cAMP are added tosample wells and incubated at room temperature for 2 h. The sample wells are then washed with PBS and p-nitrophenyl phosphate (pNp

10、p) substrate solution is added. Finally, the samples are incubated at roomtemperature for 1 h and their absorbance values are read according to the manufacturers instructions 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay 1 hAMSCs are irra

11、diated with the indicated doses of UVA and then incubated with 1 to 100 M of Vanillin forthree days under serum-free conditions (in DMEM devoid of serum, at 37C with 5% CO2). After three days,cell proliferation is measured using BrdU incorporation assay 1.MCE has not independently confirmed the accu

12、racy of these methods. They are for reference only.Animal Male Sprague-Dawley rats (200 to 250 g) are used in this study. The animals are divided into three groupsAdministration 2 with 8 to 10 rats per group: the stress+fluoxetine group; the stress+Vanillin aromatherapy group and thestress (untreate

13、d) group. For the stress+fluoxetine group, the animals are administered a daily oral dose (10mg/kg/d, diluted in distilled water) of the SSRI fluoxetine each morning. For the stress+Vanillin group and thebulbectomy+Vanillin group, Vanillin is administrated in a Plexiglas cylinder 50 cm tall and 35 c

14、m diameter2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemEwith two layers separated by a porous Plexiglas board. The rat still in its cage is gently placed on the upperlayer, and 5 mL of 600 mg/L Vanillin (in distilled water) sprayed on to the floor of the lower layer. Rats in thestress and the con

15、trol groups receive similar handling to the stress+Vanillin group, but without any odoradministrated 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Lee SY, et al. Vanillin attenuates negative effects of ultraviolet A on the stemness of human adipose tissue-derived mesenchymal stemcells. Food Chem Toxicol. 2016 Oct;96:62-9.2. Xu J, et al. Vanillin-induced amelioration of depression-like behaviors in rats by modulating monoamine neurotransmitters in the brain.Psychi