“細胞生物學(xué)”教學(xué)大綱

1、 10“細胞生物學(xué)”教學(xué)大綱四川大學(xué)生命科學(xué)學(xué)院AGeneralOutlineoftheCellBiologyCollege of Life Sciences, Sichuan University一、課程根本信息課程名稱中、英文：細胞生物學(xué) Cell Biology課程類別：專業(yè)根底課 版社，2022.6Cell and Molecular Biology: Concepts and Experiments, 6th n Karp, 2022Molecular Biology of the Cell, 5thEdition, Alberts et al., 2022學(xué)時：48學(xué)分：3課程地位

2、：國家精品課程主講教師：鄒方東、王喜忠、李娟、李沁桐、張詠梅、楊軍 等二、教學(xué)目的及要求生命科學(xué)綜合素養(yǎng)教育及試驗技能綜合培育等方面都具有重要作用。練，同時使學(xué)生學(xué)會學(xué)習(xí)，具有自我開拓可獲得學(xué)問和利用信息的力量。TeachingObjectives:Cell biology is one of the most important courses of current biology, and hasbeen listed as a key curriculum of sciences by the Ministry of Education of Chinaand excellent cur

3、riculum of Sichuan University and Sichuan province . As the basic unit of structure and function for all organisms, cell is both the start and convergepoints of life science.The teaching target of cell biology is to acquaint the students with the basic knowledge,conceptsandfundamentaltheories,aswell

4、asthebriefhistoryandmostadvanced domain of the subject being taught. Furthermore, the students are to come under trainings on their thinking to master the skills of learning, so as to be capable ofexploiting their talents on getting knowledge available and utilizing information.教學(xué)根本特色：課堂教學(xué)效果顯著，課程在國內(nèi)

5、具有很好影響力和輻射作用；生命科學(xué)進展前沿和熱點領(lǐng)域；按著學(xué)科內(nèi)容體系、學(xué)科內(nèi)在聯(lián)系和主從關(guān)系，依次確定學(xué)問塊學(xué)問單元的關(guān)系，處理好傳授學(xué)問和力量培育的關(guān)系。21Basic teaching characteristicsCell biology is one of the countrys first-class name brand courses, which is performed bilingually and employing the multi-media. The teaching effect in class is rather remarkable and the c

6、onstruction of the course is on the top internal.Frequent updating. Not only an embodiment of basic knowledge, but also areflection of preceding fields and hotspots of life sciences.Taking the police of “education should face the modernization, face the world andface the future” as the guideline. Ac

7、cording to the content system, internalconnections as well as the principal and subordinate relationships of the subject, we established the route: learning block unit point. And this has beenproved exercisable, which follows the teaching rules and scientific developmentallaws and disposes well of t

8、he relations between traditional and modern contents, and the relation between teaching knowledge and training ability as well.Leave some room for innovation, which in favor of reforms on textbook series for21stcentury and teaching substances.三、教學(xué)內(nèi)容Learning Block 1 Review of Cell Biology and Its Res

9、earchingMethods學(xué)問單元一 第一章 緒論(2學(xué)時)第一節(jié) 細胞生物學(xué)爭辯的內(nèi)容與現(xiàn)狀學(xué)問點二、了解細胞生物學(xué)的主要爭辯內(nèi)容學(xué)問點一、了解細胞的覺察學(xué)問點三、了解細胞學(xué)的經(jīng)典時期學(xué)問點五、了解細胞生物學(xué)學(xué)科的形成與進展學(xué)問點六、了解細胞生物學(xué)的主要學(xué)術(shù)組織、學(xué)術(shù)刊物與教科書Unit 1Chapter 1 IntroductionSection 1Contents and Actuality of Researches on Cell BiologyPoint 1. Know that cell biology is a fundamental subject in modern l

10、ife science Point 2 Know the important researching contents on cell biologySection 2A Brief History of Cytology and Cell BiologyPoint 1 Know the discovery of cellPoint 2 Know the establishment of cytology and its significancePoint 3 Know the classical period of cytologyPoint 4 Know the embranchment

11、and development of experimental cytology and cytologyPoint 5 Know the subjects establishment and development of cell biologyPoint 6 Know the main academic organizations, issues and textbooks學(xué)問單元二 其次章 細胞的統(tǒng)一性與多樣性(2學(xué)時)第一節(jié) 細胞的根本特征學(xué)問點二、把握細胞的根本共性其次節(jié) 原核細胞與古核細胞學(xué)問點一、了解最小最簡潔的細胞支原體學(xué)問點三、了解古核細胞古細菌學(xué)問點二、理解細胞的大小及其影

12、響因素學(xué)問點一、了解病毒的根本學(xué)問學(xué)問點二、了解病毒在細胞內(nèi)的增殖學(xué)問點三、理解病毒與細胞在起源與進化中的關(guān)系Unit2Chapter 2 Diversity and Commonality of CellsSection1 Basic Concepts of CellsPoint 1 Master and apprehend that cells are the basic units of lifePoint 2 Master the commonness of cellSection2Prokaryotic and Eukaryotic CellPoint 1 Know the mini

13、mal and simplest cellmycoplastPoint 2 Know two representation of prokaryotic cell bacteria and cyanobacteriaPoint 3 Know archaeobacteriaSection3EukaryoticCellPoint 1 Apprehend and master the basic structural system of eukaryotic cellPoint 2 Apprehend the size of cells and its regulatorsPoint 3 Appre

14、hend and master the comparison of prokaryotic and eukaryotic cellsPoint 4 Apprehend and master the comparison between plant cell and animal cellSection 4 Noncellular living OrganismVirusPoint 1 Know the basic knowledge of virusPoint 2 Know that virus proliferate (replicate) in cellsPoint 3 Apprehend

15、 the relation between virus and cell in the origin and evolution of life學(xué)問單元三 第三章 細胞生物學(xué)爭辯方法(3學(xué)時)第一節(jié) 細胞形態(tài)構(gòu)造的觀看方法學(xué)問點三、了解掃描隧道顯微鏡學(xué)問點二、了解細胞成分的化學(xué)顯示方法第三節(jié) 細胞培育與細胞工程學(xué)問點一、理解并把握熒光漂白恢復(fù)技術(shù)學(xué)問點三、理解酵母雙雜交技術(shù)學(xué)問點五、理解放射自顯影技術(shù)學(xué)問點二、了解突變體制備技術(shù)學(xué)問點三、了解蛋白質(zhì)組學(xué)技術(shù)Unit3Chapter 3 Methodology of Cell BiologySection 1 Methods on Cell St

16、ructure ObservationPoint 1 Apprehend and master of light microscopy Point 2 Apprehend and master of electron microscopePoint 3 Know scanning tunneling microscopeSection 2 Analytical Methods of Cell ComponentsPoint 1 Apprehend using super-centrifugation to separate cellular componentsPoint 2 Know the

17、 marking methods of components in cell.Point 3 Apprehend and master localization and qualitative analyses using specialprotein antigenPoint 4 Know the localization and qualitative analyses of special intracellular nucleicacidsPoint 5 Know the quantitative analyses of cytochemistry, apprehend and mas

18、ter flowcytometrySection 3 Cell Culture and Cell EngineeringPoint 1 Master types and methods of cell incubation Point2MastermainachievementsofcellengineeringSection 4 Dynamics of Cell and BiomacromoleculesPoint 1 Apprehend and master fluorescence photobleaching recoveryPoint 2 know the Single Molecu

19、le DetectionPoint 3 Apprehend yeast two-hybrid systemPoint 4 Apprehend fluorescence resonance energy transferPoint 5 Apprehend the technique to trace intracellular dynamic synthesis ofmacromolecules using radioautographySection 5 Model Organisms and Functional Genome ResearchPoint 1 Know model organ

20、isms used in cell biologyPoint 2 Know how to prepare mutantsPoint 3 Know proteome methods學(xué)問塊二 生物膜系統(tǒng)Learning Block 2Biomembrane System學(xué)問單元一 第四章 細胞質(zhì)膜 (2學(xué)時)學(xué)問點二、把握膜脂的種類及運動方式 學(xué)問點一、理解并把握膜的流淌性 學(xué)問點四、了解細胞膜的功能Unit 1Chapter 4 Cell MembraneSection 1 Structure Model of Cell Membrane and constituentsPoint 1 Mast

21、er and apprehend membrane structurePoint 2 Master types of membrane lipids and motilities Point3MastertypesofmembraneproteinsanddetergentsSection2BasicPropertiesandFunctionsofBiomembranePoint 1 Apprehend and master fluidity of membranePoint 2 Apprehend and master the asymmetry of membranePoint 3 Kno

22、w membrane associated cytoskeleton and specialized structures on cell surfacePoint 4 Know functions of membrane學(xué)問單元二 第五章 物質(zhì)的跨膜運輸 (2學(xué)時)學(xué)問點一、把握載體蛋白和通道蛋白轉(zhuǎn)運特點 其次節(jié) ATP泵、Ca2+泵工作原理與功能V F 型質(zhì)子泵的功能學(xué)問點三、把握 ABC 超家族的功能第三節(jié) 胞吞作用與胞吐作用學(xué)問點一、理解并把握吞噬作用過程與功能學(xué)問點三、了解其他類型的胞飲作用學(xué)問點四、理解并把握胞吞作用對信號轉(zhuǎn)導(dǎo)的下調(diào)或激活Unit2Chapter 5 The Mov

23、ement of Substances Across Cell Membrane Section 1 Membrane Transport Proteins and Transport Across CellMembrane of small moleculesPoint 1 Master the characters of carrier proteins and channel proteinsPoint 2 Master the types and features of small molecules across cell membraneSection 2 ATP-driven P

24、umps and Active TransportPoint 1 Apprehend and master the mechanisms and functions of the Na +-K+ pump andCa2+ pumpPoint 2 Master the functions fo V-type pump and F-type pumpPoint 3 Master the functions of ABC superfamilyPoint 4 Know the relations between ions transport and membrane potentialSection

25、3EndocytosisandExocytosisPoint 1 Apprehend and master the process and functions of phagocytosisPoint2 Apprehend andmaster theprocess and features ofclathrin dependentendocytosisPoint 3 Know other types of pinocytosisPoint 4 Apprehend and master the regulations of endocytosis to signaling學(xué)問單元三 第六章 線粒

26、體和葉綠體(2學(xué)時)第一節(jié) 線粒體與氧化磷酸化學(xué)問點一、理解并把握線粒體的形態(tài)與動態(tài)變化學(xué)問點三、理解并把握線粒體能量轉(zhuǎn)換學(xué)問點四、了解線粒體與疾病自學(xué)學(xué)問點二、了解葉綠體的構(gòu)造和化學(xué)組成第三節(jié) 線粒體和葉綠體的半自主性及其起源學(xué)問點一、了解線粒體和葉綠體的 DNA學(xué)問點二、理解線粒體和葉綠體的蛋白質(zhì)合成學(xué)問點四、了解線粒體和葉綠體的增殖與起源Unit3Chapter 6 Mitochondria and ChloroplastSection1MitochondrionandOxidativePhosphorylationPoint 1 Apprehend and master the mor

27、phology and dynamics of mitochondrionPoint 2 Know the chemical composition and localization of enzymes in mitochondrionPoint 3 Apprehend and master functions of mitochondrion for energy conversion Point 4 Know mitochondrion and diseasesSection2ChloroplastandPhotosynthesis(Self-study)Point 1 Know the

28、 modalities, sizes and number of chloroplast Point2KnowstructureandchemicalcompositionofchloroplastPoint3ApprehendandmastermainfunctionofchloroplastphotosynthesisSection 3 Mitochondrion and Chloroplast are Semiautonomous Organelles ，and OriginPoint 1 Know mitochondrion and chloroplast DNAPoint 2 App

29、rehend the protein synthesis of mitochondrion and chloroplastPoint 3 Apprehend the relation between mt/chDNA and nuclear genome Point 4 Know proliferation and origin of mitochondrion and chloroplast學(xué)問單元四 第七章 細胞質(zhì)基質(zhì)與內(nèi)膜系統(tǒng)(3學(xué)時)學(xué)問點一、理解把握內(nèi)質(zhì)網(wǎng)蛋白質(zhì)合成、修飾加工、應(yīng)激反響學(xué)問點三、理解并把握溶酶體類型、功能、發(fā)生以及疾病學(xué)問點四、了解過氧化物酶體與溶酶體的區(qū)分、功能與發(fā)

30、生5 第八章 蛋白質(zhì)分選與膜泡運輸(3 學(xué)時)學(xué)問點二、理解并把握蛋白質(zhì)分選途徑與類型其次節(jié) 細胞內(nèi)膜泡運輸學(xué)問點二、了解包被蛋白種類學(xué)問點四、了解膜泡的轉(zhuǎn)運、錨定與融合學(xué)問點五、了解細胞構(gòu)造體系的組裝Unit4Chapter 7 Cytosol and Endomembrane SystemSection 1 Cytoplasmic Matrix (Cytosol) and its functionsPoint 1 Apprehend the meaning of cytoplasmic matrixPoint 2 Master functions of cytoplasmic matrix

31、Section 2 Endomembrane System and Its FunctionPoint 1 Apprehend and master protein synthesis, modification and stress response in ER Point2ApprehendandmasterthepolarityofGolgicomplexanditsrolesinproteinsecretion,modification and proteolysisPoint 3 Apprehend and master lysosoms types, functions, biog

32、enesis, and its role in diseases Point4Knowthedifferenceoflysosomeandperoxisome,andthefunctionsandbiogenesisofperoxisomeUnit5Chapter 8 Protein Sorting and Vesicular TransportingSection 1 Protein SortingPoint 1 Apprehend and master signal hypothesis and sorting signal Point2Apprehendandmasterpathways

33、andtypesofproteinsortingPoint 3 Know protein sorting into mitochondrion, chloroplast, and peroxisomeSection 2 Vesicular Transporting in the CellPoint 1 Apprehend and master relations of structure and function for endomembrane organellesPoint 2 Know the types of coating proteinsPoint 3 Apprehend the

34、roles of 3 types of coating proteins in vesicular assembly andtransportingPoint 4 Know the process of vesicular transporting, docking and fusionPoint 5 Know the assembly of subcellular structures6 第九章 細胞信號轉(zhuǎn)導(dǎo)3第一節(jié) 細胞信號轉(zhuǎn)導(dǎo)概述學(xué)問點一、了解細胞通信類型其次節(jié) 細胞內(nèi)受體介導(dǎo)的信號轉(zhuǎn)遞學(xué)問第三節(jié)、G 蛋白偶聯(lián)受體介導(dǎo)的信號傳遞G 蛋白偶聯(lián)受體介導(dǎo)的信號通路信號通路PI3K-PKB 信號

35、通路學(xué)問點三、了解其他類型信號通路第五節(jié) 其他細胞外表受體介導(dǎo)的信號通路信號通路NF-kB 信號通路Notch信號通路第六節(jié) 細胞信號轉(zhuǎn)導(dǎo)的整合與把握學(xué)問點二、理解蛋白激酶的網(wǎng)絡(luò)整合信息學(xué)問點三、理解并把握信號的把握：受體脫敏與下調(diào)Unit6Chapter 9 Cell Signal Transduction Section 1 Overview of Cell SignalingPoint 1 Know types of cell signalingPoint 2 Master types of signal molecules and receptorsPoint 3 Apprehend

36、and master characteristics of signal transductionSection2SignalTransductionbyIntracellularReceptorsPoint 1 Know nuclear receptors and functionsPoint 2 Master signal transduction by NOSection 3 Signal Transduction by G Protein-Coupled ReceptorsPoint 1 Apprehend and master the domain and activation of

37、 G protein-coupledreceptorsPoint 2 Apprehend signaling pathways mediated by G protein-coupled receptorsSection 4 Signal Transduction by Enzyme-coupled ReceptorsPoint 1 Apprehend the receptor tyrosine kinase and RTK-Ras signal pathwayPoint 2 Apprehend signal pathway mediated by PI3K-PKB(Akt)Point 3 K

38、now other types of signal pathwaysSection 5 Signal Pathways by Other Receptors on Cell SurfacePoint 1 Know the Wnt-beta-catenin signaling pathwayPoint 2 Know the Hedghog signaling pathwayPoint 3 Know the NF-kB signaling pathwayPoint 4 Know the Notch signaling pathwayPoint 5 Know signaling pathway me

39、diated by integrinsSection 6 The Convergence and Regulation of Signal TransductionPoint 1 Apprehend and master the features of signal responses Point2ApprehendtheConvergenceofthecross-talkofsignalingPoint 3 Apprehend the desensitization and down-regulation of receptors學(xué)問塊三 細胞骨架系統(tǒng)Learning Block 3The

40、Cytoskeleton System學(xué)問單元一 第十章 細胞骨架4學(xué)問點一、理解并把握微絲裝配動態(tài)性學(xué)問點三、把握微絲的分子馬達種類學(xué)問點四、理解并把握肌肉收縮的分子機制學(xué)問點二、把握微觀組織者中心類型學(xué)問點五、把握纖毛和鞭毛的構(gòu)造、功能及運動機制學(xué)問點一、了解中間絲的類型學(xué)問點三、把握中間絲功能Unit1Chapter 10 The CytoskeletonSection 1 Microfilaments and Cell MotilityPoint 1 Apprehend and master the dynamic assembly of microfilamentsPoint 2 A

41、pprehend and master the types and functions of microfilament-associatedproteins and cell motilityPoint 3 Master the types of microfilament associated motor proteinsPoint 4 Apprehend and master molecular mechanism of muscle contractionSection 2 MicrotubulesPoint 1 Apprehend and master the polarity an

42、d dynamic properties of microtubulesPoint 2 Master types of microtubule-organizing centersPoint 3 Apprehend and master the regulation of microtubules by microtubule-associatedproteinsPoint 4 Apprehend and master motor proteins and intracellular transportPoint 5 Master the structure, function and mot

43、ility mechanism of cilia and flagellaSection 3 Intermediate FilamentsPoint 1 Master types of intermediate filamentsPoint 2 Master the assembly and disassembly and tissue distribution of intermediate filamentsPoint 3 Master the function of intermediate filamentsLearning Block 4 Genetic Information Ca

44、rrier and Expression System學(xué)問單元一 第十一章 細胞核與染色質(zhì)(5學(xué)時)第一節(jié) 核被膜學(xué)問點三、把握核纖層功能學(xué)問點一、把握染色質(zhì)的概念及化學(xué)組成學(xué)問點三、理解染色質(zhì)包裝的構(gòu)造模型第三節(jié) 染色質(zhì)的復(fù)制與表達學(xué)問點一、了解染色質(zhì)復(fù)制與修復(fù) 學(xué)問點四、了解染色質(zhì)與表觀遺傳學(xué)問點一、了解中期染色體的形態(tài)構(gòu)造學(xué)問點三、了解核型與染色體顯帶第五節(jié) 核仁與核體學(xué)問點一、把握核仁的超微構(gòu)造 學(xué)問點三、了解核仁的周期學(xué)問點四、了解核體Unit1Chapter 11 Nucleus and ChromatinsSection1NuclearEnvelopePoint 1 Master

45、 structure and dynamics of nuclear envelopePoint 2 Apprehend and master functions of nuclear pore complexPoint 3 Master the function of nuclear laminaSection2ChromatinPoint 1 Master concept and chemical composition of chromosome Point 2 Master the basic structure unit of chromosomenucleosomePoint 3

46、Apprehend structural model of chromosome packagePoint 4 Master of euchromatin and heterochromatinSection 3 Replication and Expression of ChromatinsPoint 1 Know the replication and repairmen of chromatins Point 2 Know the activation and inactivation of chromatins Point3Knowtheregulationofgeneexpressi

47、onbychromatinsSection4ChromosomesPoint 1 Know modal structure of metaphase chromosomePoint 2 Apprehend and master 3 functional elements of chromosome DNAPoint 3 Know karyotype and bandingPoint 4 Apprehend cause and function of giant chromosomeSection 5 The Nucleolus and The Nuclear BodyPoint 1 Maste

48、r ultrastructure of nucleolusPoint 2 Apprehend and master functions of nucleolusPoint 3 Know nucleolar cyclePoint 4 Know the nuclear bodies學(xué)問單元二 第十二章 核糖體1第一節(jié) 核糖體的類型與構(gòu)造學(xué)問點二、把握核糖體的構(gòu)造其次節(jié) 多聚核糖體與蛋白質(zhì)的合成學(xué)問點一、了解多聚核糖體 學(xué)問點三、了解 RNA 在生命起源中的地位Unit2Chapter11Ribosome(Self-study)Section 1 Types and Structure of Rib

49、osome Point 1 Master basic types and composition of ribosomePoint 2 Master structure of ribosomePoint 3 Master functions ribosomal proteins and rRNASection2PolyribosomeandProteinSynthesisPoint 1 Know polyribosome Point2MasterproteinsynthesisPoint 3 Know position of RNA in life originLearning Block 5

50、 Cell Proliferation, Differentiation and CellSenescence and Apoptosis學(xué)問單元一 第十三章 細胞周期與細胞分裂 (3 學(xué)時)學(xué)問點二、把握細胞周期同步化的方法原理 學(xué)問點三、把握早期胚胎細胞的細胞周期學(xué)問點二、理解并把握動物細胞胞質(zhì)分裂分子調(diào)控機制學(xué)問點四、把握減數(shù)分裂的特別構(gòu)造及其變化Unit1Chapter 13 Cell Cycle and Cell DivisionSection 1 Cell CyclePoint 1 Master cell cycle concepts and cycling phrase even

51、ts Point 2 Master principle of cell cycle synchronization methodPoint 3 Master the cell cycle of the early frog embryoSection 2 Cell DivisionPoint 1 Apprehend and master events and apparatuses in cell cycle phasesPoint 2 Apprehend and master the mechanism of animal cytokinesis Point 3 Apprehend the

52、process and significances of meiosisPoint 4 Master the special apparatus and its dynamic of meiosis學(xué)問單元二 第十四章 細胞增殖調(diào)控與癌細胞 (3 學(xué)時)學(xué)問點一、理解 MPF 的覺察及其作用MPF學(xué)問點三、把握周期蛋白的作用學(xué)問點四、把握 CDK 激酶和 CDK學(xué)問點五、理解并把握細胞周期如何正常有序的運轉(zhuǎn)及調(diào)控 學(xué)問點一、把握癌細胞的根本特征學(xué)問點三、理解腫瘤發(fā)生是基因突變積存的結(jié)果學(xué)問點四、理解并把握腫瘤干細胞Unit2Chapter 14 Cell Cycle Control and C

53、ancer CellsSection 1 Cell Cycle ControlPoint 1 Apprehend the discovery and functions of MPFof P 34cdc2 kinase and its relation with MPFPoint 3 Master functions of cyclic proteinPoint 4 Master CDK kinase and CDK inhibitorsPoint 5 Master and apprehend how cell cycle function properly and its regulatio

54、nPoint 6 Know other inner and outer factors in cell cycle regulationSection2CancerCellPoint 1 Master basic features of cancer cellsPoint 2 Master relations between cancers and oncogenes and tumor-suppressor genesPoint 3 Apprehend cancers results from accumulation of gene mutationPoint 4 Apprehend an

55、d master cancer stem cells學(xué)問單元三 第十五章 細胞分化與胚胎發(fā)育 (3 學(xué)時)學(xué)問點一、把握細胞分化的根本概念以及去分化和再生學(xué)問點三、了解影響細胞分化的因素學(xué)問點一、了解生殖細胞的分化學(xué)問點二、了解早期胚胎發(fā)育過程中的細胞分化Unit3Chapter15CellDifferentiationandEmbryonicDevelopmentSection 1 Cell DifferentiationPoint 1 Master basic concepts of cell differentiation, dedifferentiation and re-differ

56、entiationPoint 2 Know factors influencing cell differentiationPoint 3 Apprehend and master totipotency and stem cell Point4KnowcelldifferentiationandembryodevelopmentSection2CellDifferentiationinEmbryonicDevelopmentPoint 1 Know the differentiation of germ cellsPoint 2 Know the cell differentiation d

57、uring early embryonic development學(xué)問單元四 第十六章 細胞死亡與細胞年輕(3學(xué)時)第一節(jié) 細胞死亡學(xué)問點一、把握細胞死亡類型學(xué)問點二、理解并把握細胞凋亡的概念學(xué)問點三、把握細胞凋亡的形態(tài)學(xué)、生物化學(xué)特征及檢測方法學(xué)問點四、理解細胞凋亡的生理學(xué)及醫(yī)學(xué)意義學(xué)問點五、理解并把握細胞凋亡的分子機制及主要凋亡通路 學(xué)問點七、理解自噬性細胞死亡的分子機制其次節(jié) 細胞年輕Hayflick學(xué)問點三、理解并把握細胞年輕的分子機制：端粒學(xué)說和氧化損傷學(xué)說Unit4Chapter 15 Cell Death and Cell SenescenceSection1Cell DeathPoint 1 Master the types o