常用熒光染料的激發(fā)及發(fā)射波長(zhǎng)

熒光素名稱激發(fā)波長(zhǎng)(nm)發(fā)射波長(zhǎng)(nm)備注1,5IAEDANS336490

1,8-ANS372480

4-Methylumbelliferone385502

5-carboxy-2,7-dichlorofluorescein504529

5-Carboxyfluorescein(5-FAM)492518

5-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)542568

5-FAM(5-Carboxyfluorescein)492518

5-HAT(HydroxyTryptamine)370-415520-540

5-HydroxyTryptamine(HAT)370-415520-540

5-ROX(carboxy-X-rhodamine)578

567604

591

5-TAMRA(5-Carboxytetramethylrhodamine)548

542552

568

6-Carboxyrhodamine6G518543

6-CR6G518543

6-JOE520548

7-Amino-4-methylcoumarin351430

7-AminoactinomycinD(7-AAD)546647

7-Hydroxy-4-methylcoumarin360449,455

9-Amino-6-chloro-2-methoxyacridine412,430471,474

ABQ344445

AcidFuchsin540630

ACMA(9-Amino-6-chloro-2-methoxyacridine)412,430471,474

AcridineOrange+DNA502526

AcridineOrange+RNA460650

AcridineOrange,bothDNA&RNA440-480520-650

AcridineRed455-600560-680

AcridineYellow470550

Acriflavin436520

AcriflavinFeulgenSITSA355-425460

Aequorin(Photoprotein)

466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFP

AlexaFluor350?346

342442

441

AlexaFluor430?431540

AlexaFluor488?495,492519,520

AlexaFluor532?531,532553,554

AlexaFluor546?556,557572,573

AlexaFluor568?577,578603

AlexaFluor594?590,594617,618

AlexaFluor633?632650

AlexaFluor647?647666

AlexaFluor660?668698

AlexaFluor680?679702

AlizarinComplexon530-560,580580,624-645

AlizarinRed530-560580

Allophycocyanin(APC)630,645655,660

AMC,AMCA-S345445

AMCA(Aminomethylcoumarin)345

347425

444

AMCA-X353442

AminoactinomycinD555655

Aminocoumarin346

350442

445

Aminomethylcoumarin(AMCA)345

347425

444

AnilinBlue

600

Anthrocylstearate360-381446

APC(Allophycocyanin)630,645655,660

APC-Cy7625-650755

APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505

AstrazonBrilliantRed4G500585

AstrazonOrangeR470540

AstrazonRed6B520595

AstrazonYellow7GLL450480

Atabrine436490

ATTO-TAG?CBQCA465560

ATTO-TAG?FQ486591

Auramine460550

AurophosphineG450580

Aurophosphine450-490515

BAO9(Bisaminophenyloxadiazole)365395

BCECF(highpH)492,503520,528

BCECF(lowpH)482520

BerberineSulphate430550

BetaLactamase409447,520

CoelenterazineO460575

CoumarinPhalloidin387470

C-phycocyanine

CPMMethylcoumarin384469MethylcoumarinCTC400-450602

CTCFormazan

Cy2?489506

Cy3.18554568

Cy3.5?581598

Cy3?514

552

554566

570

Cy5.18649666

Cy5.5?675695

Cy5?649666

670

Cy7?710,743767,805

CyanGFP433(453)475(501)

cyclicAMPFluorosensor(FiCRhR)500517

CyQuantCellProliferationAssay480520CellProliferationAssayDabcyl453

Dansyl340578

DansylAmine337517

DansylCadaverine335518

DansylChloride372518

DansylDHPE336517

Dansylfluoride356none

DAPI359461

Dapoxyl403580

Dapoxyl2374574

Dapoxyl3373574

DCFDA504529

DCFH(DichlorodihydrofluoresceinDiacetate)505535

DDAO463607

DHR(Dihydorhodamine123)505534

Di-4-ANEPPS496705

Di-8-ANEPPS(non-ratio)488

498605

713

DiA(4-Di-16-ASP)456591

DichlorodihydrofluoresceinDiacetate(DCFH)505535

DiD-LipophilicTracer644665LipophilicTracerDiD(DiIC18(5))644665

DIDS341415

Dihydorhodamine123(DHR)505535

DiI(DiIC18(3))549,551565

Dinitrophenol349

DiO(DiOC18(3))484,487501,502

DiR748780LipophilicTracerDiR(DiIC18(7))750779

DM-NERF(highpH)497/510540RatioDye,pHDNP349

Dopamine340490-520

DsRed558583RedfluorescentproteinDTAF494520

DY-630-NHS621660Hemicyane

labelforproteinsandDNADY-635-NHS634664Hemicyane

labelforproteinsandDNAEBFP383447EnhancedBlueFluorescentProteinECFP436474EnhancedCyanFluorescentProteinEGFP488,498507,516EnhancedGreenFluorescentProteinELF97345530

Eosin524545

Erythrosin529,532554,555

ErythrosinITC529555

EthidiumBromide510,523595,605

Ethidiumhomodimer-1(EthD-1)528617

Euchrysin430540

EukoLight

Europium(III)chloride

EYFP513,520527,532EnhancedYellowFluorescentProteinFastBlue360440

FDA494520

Feulgen(Pararosaniline)570625

FIF(FormaldehydInducedFluorescence)405433

FITC490,494520,525

FITCAntibody493517

FlazoOrange375-530612

Fluo-3480–506,506520,527

Fluo-4494516

Fluorescein(FITC)490,494520,525

FluoresceinDiacetate494520

Fluoro-Emerald495524

Fluoro-Gold(Hydroxystilbamidine)361536

Fluor-Ruby555582

FluorX494520

FM1-43?479598

FM4-46515640

FuraRed?(highpH)572657

FuraRed?/Fluo-3

Fura-2,highcalcium335505ExcitationratiodyeFura-2,lowcalcium363512ExcitationratiodyeFura-2/BCECF

GenacrylBrilliantRedB520590

GenacrylBrilliantYellow10GF430485

GenacrylPink3G470583

GenacrylYellow5GF430475

GeneBlazer(CCF2)

GFP(S65T)498516

GFPredshifted(rsGFP)498516

GFPwildtype,non-UVexcitation(wtGFP)475509

GFPwildtype,UVexcitation(wtGFP)395509

GFPuv385508

GloxalicAcid405460

GranularBlue355425

Haematoporphyrin530-560580

Hoechst33258345487

Hoechst33342347483

Hoechst34580392440

HPTS355465

Hydroxycoumarin325-360386-455

Hydroxystilbamidine(FluoroGold)361536

Hydroxytryptamine400530

Indo-1,highcalcium330401EmissionratiodyeIndo-1,lowcalcium346475EmissionratiodyeIndodicarbocyanine(DiD)644665

Indotricarbocyanine(DiR)748780

IntrawhiteCf360430

JC-1514529

JO-JO-1530545

JO-PRO-1532544

LaserPro795812

Laurodan355460

LDS751(DNA)543712

LDS751(RNA)590607

LeucophorPAF370430

LeucophorSF380465

LeucophorWS395465

LissamineRhodamine572,577591,592

LissamineRhodamineB577592

LIVE/DEADKitAnimalCells

Calcein/Ethidiumhomodimer494

528517

617formoredetailsrefer

toLOLO-1566580

LO-PRO-1568581

LuciferYellow425,428528,536,540

LysoTrackerBlue373422

LysoTrackerBlue-White466536

LysoTrackerGreen504,534511,551

LysoTrackerRed490516

LysoTrackerYellow551576

LysoSensorBlue374424

LysoSensorGreen442505

LysoSensorYellow/Blue384540

MagGreen507531

MagdalaRed(PhloxinB)524600

Mag-FuraRed483/427659/631RatioDye,Mg2+Mag-Fura-2369/329

369/330508

511/491RatioDyeCa2+

RatioDyeMg2+Mag-Fura-5369/330

369/332505/500

505/482RatioDye,Ca2+

RatioDye,Mg2+Mag-Indo-1349/328

349/330480/390

480/417RatioDye,Ca2+

RatioDye,Mg2+MagnesiumGreen506,507531

MagnesiumOrange550575

MalachiteGreen628

MarinaBlue362459

MaxilonBrilliantFlavin10GFF450495

MaxilonBrilliantFlavin8GFF460495

Merocyanin555578

Methoxycoumarin360410

MitotrackerGreenFM490516

MitotrackerOrange551576

MitotrackerRed578599

Mitramycin450470

Monobromobimane398490

Monobromobimane(mBBr-GSH)398500

Monochlorobimane380461

MPS(MethylGreenPyronineStilbene)364395

NBD466539

NBDAmine450530

NileRed515-555,559590,640

Nitrobenzoxadidole465510-650

Noradrenaline340490-520

NuclearFastRed289-530580

NuclearYellow365495

NylosanBrilliantIavinE8G460510

OregonGreen503522

OregonGreen488-X494517

OregonGreen?503522

OregonGreen?488490,493514,520

OregonGreen?500497517

OregonGreen?514506526

PacificBlue405455

Pararosaniline(Feulgen)570625

PBFI340/380420ExcitationratiodyePE-Cy5488670

PE-Cy7488755,767

PerCP488675

PerCP-Cy5.5488710

PE-TexasRed[Red613]488613

PhloxinB(MagdalaRed)524600

Phorwite360430

PhorwiteBKL370430

PhorwiteRev380430

PhorwiteRPA375430

Phosphine3R465565

PhotoResist365610

PhycoerythrinB[PE]546-565575

PhycoerythrinR[PE]565578

PKH26(Sigma)551567

PKH67496520ChromaPMIA341376

PontochromeBlueBlack535-553605

POPO-1433457

POPO-3533574

PO-PRO-1435455

PO-PRO-3539567

Primuline410550

ProcionYellow470600

PropidiumIodid(PI)(305),536,538617

PyMPO412,415561,564,570

Pyrene360387

Pyronine410540

PyronineB540-590560-650

PyrozalBrilliantFlavin7GF365495

QSY7560

QuinacrineMustard440510

Red613[PE-TexasRed]488613

Resorufin571584,585

RH414532716

Rhod-2552576

Rhodamine550573

Rhodamine110496,497520

Rhodamine123507529

Rhodamine5GLD470565

Rhodamine6G525555

RhodamineB540625

RhodamineB200523-557595

RhodamineBextra550605

RhodamineBB540580

RhodamineBG540572

RhodamineGreen502527

RhodaminePhallicidine558,542575,565

RhodaminePhalloidine542565

RhodamineRed570590

RhodamineWT530555

RoseBengal525,540550-600

R-phycocyanine

R-phycoerythrin(PE)565578

rsGFP473509redshiftedGFP(S65T)S65A471504

S65C479507

S65L484510

S65T488511

SapphireGFP395511

SBFI340/380420ExcitationratiodyeSerotonin365520-540

SevronBrilliantRed2B520595

SevronBrilliantRed4G500583

SevronBrilliantRedB530590

SevronOrange440530

SevronYellowL430490

sgBFP?387450

sgBFP?(superglowBFP)387450Quantum’sSuperGlo?GFP

AFPssgGFP?474488

sgGFP?(superglowGFP)474509Quantum’sSuperGlo?GFP

AFPsSITS336436IonChannelsSITS(Primuline)395-425450

SITS(StilbeneIsothiosulphonicAcid)365460

SNAFLcalcein506/535535/620RatioDye,pHSNAFL-1508/540543/623RatioDye,pHSNAFL-2514/543546/630RatioDye,pHSNARFcalcein552/574590/629RatioDye,pHSNARF1576/548635/587ExcitationandemissionratiodyeSodiumGreen506,507532Na+,K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30,509/31538/44,524/56VysisSpectrumOrange559/38,560588/48VysisSpectrumRed587,587/35612,612/51

SPQ(6-methoxy-N-(3-sulfopropyl)quinolinium)344443

Stilbene335440

SulphorhodamineBcanC520595

SulphorhodamineGExtra470570

SYTO11508,510527,530DyeforDNA,RNASYTO12499,500522,519DyeforDNA,RNASYTO13488,491509,514DyeforDNA,RNASYTO14517,521549,547DyeforDNA,RNASYTO15516,518546,555DyeforDNA,RNASYTO16488,494518,525DyeforDNA,RNASYTO17621634DyeforDNASYTO18490,493507,527DyeforDNA,RNASYTO20512530DyeforDNASYTO21494517DyeforDNASYTO22515535DyeforDNASYTO23499520DyeforDNASYTO24490515DyeforDNASYTO25521556DyeforDNASYTO40420441DyeforDNASYTO41430454DyeforDNASYTO42433460DyeforDNASYTO43436467DyeforDNASYTO44446471DyeforDNASYTO45452484DyeforDNASYTO59622645DyeforDNASYTO60652678DyeforDNASYTO61628645DyeforDNASYTO62652676DyeforDNASYTO63657673DyeforDNASYTO64599619DyeforDNASYTO80531545NucleicAcidStainSYTO81530544NucleicAcidStainSYTO82541560NucleicAcidStainSYTO83543559NucleicAcidStainSYTO84567582NucleicAcidStainSYTO85567583NucleicAcidStainSYTOXBlue445470NucleicAcidStainSYTOXGreen504523NucleicAcidStainSYTOXOrange547570NucleicAcidStainTetracycline390-425525-560

Tetramethylrhodamine(TRITC)555576

TexasRed?595620

TexasRed-X?conjugate595615

Thiadicarbocyanine(DiSC3)651,653674,675

ThiazineRedR596615

ThiazoleOrange510530

Thioflavin5430550

ThioflavinS430550

ThioflavinTCN350460

Thiolyte370-385477-488

ThiozoleOrange453480

TinopolCBS(CalcofluorWhite)390430

TMR550573

TO-PRO-1515531

TO-PRO-3644657

TO-PRO-5747770

TOTO-1514531,533

TOTO-3642660

TriColor(PE-Cy5)(488)650667

TRITCTetramethylRodamineIsoThioCyanate550573

TrueBlue365425

TruRed490695

Ultralite656678

UranineB420520

UvitexSFC365435

wtGFP395(475)508wildtypeGFPWW781605639

X-Rhodamine580605

XRITC582601

XyleneOrange546580

Y66F360508

Y66H360442

Y66W436485

YellowGFP513527Yellowshifted

GreenFluorescentProteinYFP513,520527,532YellowFluorescentProteinYO-PRO-1491506

YO-PRO-3613629

YOYO-1491508,509

YOYO-3612631

常用熒光染料的激發(fā)及發(fā)射波長(zhǎng)Fluorescent

Dye（熒光染料）Excitation（激發(fā)波長(zhǎng)nm

）Emission

發(fā)射波長(zhǎng)nm

）

Cy2

TM

489

506

GFP(Red

Shifted)

488

507

YO-PRO

TM

-1

491

509

YOYO

TM

-1

491

509

Calcein

494

517

FITC

494

518

FluorX

TM

494

519

Alexa

TM

488

490

520

Rhodamine

110

496

520

ABI,5-FAM

494

522

Oregon

Green

TM

500

503

522

Oregon

Green

TM

488

496

524

RlboGreen

TM

500

525

Rhodamine

Green

TM

502

527

Rhodamine123

507

529

Magnesium

Green

TM

506

531

Calcium

Green

TM

506

533

TO-PRO

TM

-1