第15章 志賀菌屬

1、Medical Microbiology,Department of Microbiology, HMU,第二篇,致病性細菌,第,15,章,志賀菌屬,Shigella,微生物學教研室,張文莉,Department of Microbiology, Harbin Medical University,教學大綱,?,熟悉,?,志賀菌屬生物學性狀特點（抗原構(gòu)造及分類）,?,志賀菌的致病物質(zhì)及所致疾病,?,志賀菌感染的微生物學檢查法,?,掌握,?,志賀菌的防治原則,Department of Microbiology, Harbin Medical University,問題,?,志賀菌屬的致病物質(zhì)及

2、其作用機制是什么？,?,對可疑痢疾患者如何進行微生物學檢查？,Department of Microbiology, Harbin Medical University,生物學性狀,?,形態(tài)染色,?,G,-,桿菌，,0.5-0.7 ,m,?,2-3,m,?,無鞭毛，無莢膜，,有菌毛,Department of Microbiology, Harbin Medical University,生物學性狀,?,培養(yǎng)及生化反應,?,營養(yǎng)要求不高,?,不分解乳糖,?,ss,選擇培養(yǎng)基,?,克氏雙糖管,Department of Microbiology, Harbin Medical Universit

3、y,抗原構(gòu)造及分類,?,由,O,和,K,抗原組成,?,分類：,菌種,群,型,亞型,?,痢疾志賀菌,A 1,10 8a,、,8b,、,8c,?,福氏志賀菌,B 1,6,、,x 1a,、,1b,、,2a,、,2b,y,變型,3a,、,3b,、,3c,、,4a,、,4b,?,鮑氏志賀菌,C 1,18,?,宋內(nèi)志賀菌,D 1,Department of Microbiology, Harbin Medical University,?,比其他腸道桿菌弱,?,對酸和一般消毒劑敏感,抵抗力,Department of Microbiology, Harbin Medical University,致病物質(zhì)

4、,?,侵襲力,?,內(nèi)毒素,?,外毒素,Department of Microbiology, Harbin Medical University,所致疾病,?,菌痢,?,患者和帶菌者,?,糞,-,口,?,表現(xiàn)：,?,潛伏期數(shù)小時至,7d,?,發(fā)熱、腹痛、腹,瀉、里急后重、,粘液膿血便,Department of Microbiology, Harbin Medical University,痢疾患兒,Department of Microbiology, Harbin Medical University,免疫性,?,免疫期短，不穩(wěn)固,?,免疫主要是消化道粘膜的,SIgA,Department of Microbiology, Harbin Medical University,微生物學檢查法,?,標本,?,分離培養(yǎng)與鑒定,?,毒力試驗,?,快速診斷,?,免疫熒光菌球法,?,協(xié)同凝集試驗,?,膠乳凝集試驗,?,PCR,、雜交,Department of Microbiology, Harbin Medical University,防治原則,?,隔離患者、徹底治療,?,試用的口服菌苗,?,抗生素,Department of Microbiology, Harbin Med