來料檢驗規(guī)范3

1、 杜仲葉論文：一種新型杜仲沖劑的生產(chǎn)工藝研究【中文摘要】杜仲(Eucommia ulmiodes Oliv.)為單屬科單種屬植物,其皮和葉中含有多種藥用成分,如綠原酸、黃酮、桃葉珊瑚苷,多種維生素、氨基酸,豐富的礦物質(zhì)鐵、鈣、鉀、鋅、鎂、硒等天然植物微量元素,具有調(diào)節(jié)血壓、血脂和血糖,抗氧化、抗衰老及抗腫瘤,增強免疫力,減肥美容等保健作用。本研究對杜仲有效成分提取工藝進行了深入的研究,優(yōu)化了提取工藝,提取出了富含生物活性物質(zhì)的浸膏。在此基礎(chǔ)上,研究了杜仲沖劑的造粒工藝,為新型杜仲保健品和藥品的開發(fā)提供理論依據(jù)。研究結(jié)果如下:一、杜仲葉有效成分提取工藝以杜仲葉中的生物活性物質(zhì)綠原酸和總黃酮為指標

2、,利用響應面法優(yōu)化提取杜仲葉中的工藝參數(shù)。在分析單因素的基礎(chǔ)上,以綠原酸和總黃酮的得率為響應值,以乙醇濃度(%)、提取溫度()、提取時間(h)、料液比為考察因素,并采用響應面設(shè)計實驗方法優(yōu)化提取工藝,其最優(yōu)條件為乙醇濃度55%、提取溫度63、提取時間2.2h、料液比為1:13。在此條件下,綠原酸和總黃酮的得率分別達0.81%和0.51%。二、杜仲沖劑的制備工藝通過一系列的單因素試驗選擇不同的矯味劑、賦型劑、潤濕劑,并從實際生產(chǎn)中常用的制粒方法濕法制粒、快速攪拌制粒、流化噴霧制粒中選擇快速攪拌制粒為主要的制粒方法。以粒度不合格率為響應值,確定最佳提取工藝參數(shù)為:甜菊糖苷用量為杜仲提取物的0.78

3、%、-環(huán)狀糊精與藥粉質(zhì)量之比為6,乙醇濃度為65%,乙醇用量(L)與藥粉量(kg)比例為l6,干燥溫度為70,在此條件下,杜仲沖劑的顆粒不合格率為僅為6.12%。通過本課題的研究,得到了完整的一套杜仲沖劑制備工藝:杜仲葉經(jīng)除塵、除雜,并于105殺青10min后晾干,粉碎,加入55%乙醇,乙醇用量(L)與杜仲葉(kg)比例為131,在63的溫度下提取2.2 h后,在60下濃縮、干燥,得到杜仲提取物干粉,粉碎,加用量為杜仲提取物干粉的0.78%的甜菊糖苷、6倍于其他藥粉重量的-環(huán)狀糊精、并加入濃度為65%的乙醇,乙醇用量(L)與藥粉量(kg)比例為l6,混合均勻后,倒入快速攪拌制粒機中制粒,而后在

4、70下進行干燥,時間為20 min,成型后即為杜仲沖劑?！居⑽恼縀ucommia ulmiodes Oliv. is a plant species with mono genus and family. The bark has been a traditional Chinese medicine for thousands of years. Modern scientific research has confirmed that the functional constituents in the leaf are similar to the bark. Based on th

5、is finding, the leaf has been listed in China Pharmacopoeia with therapeutic effects similar to the bark. The leaf contains a variety of medicinal ingredients, such as chlorogenic acid, flavonoids, aucubin, vitamin, amino acids, and minerals. The medical functions of the leaf include regulating bloo

6、d pressure, lipids and glucose, anti-oxidation, anti-aging, anti-tumor, enhancing immunity, beautifying skin and anti-obesity.In order to make health-care products from the leaves of E. ulmoides, an experiment was conducted on the optimization of the extraction parameters of active ingredients the g

7、ranulation process of the extracts which are rich in functional constituents. The results are as follows.1. Extraction of active ingredients of leaves of Eucommia ulmoidesResponse surface methodology (RSM) was employed to optimize the extraction parameters of chlorogenic acid and flavonolds from the

8、 leaves of E. ulmoides. Based on one-factor experiment, the effects of four independent variables, namely the concentration of ethanol (%) extraction temperature (°C), time (h) and the ratio of material to solvent on the extraction yields of chlorogenic acid and flavonoids were investigated, an

9、d then the response surface analysis was used to optimize conditions. The optimal extraction parameters were: ethanol concentration: 55%, extraction temperature: 63°C, time of extraction: 2.2h and material to solvent 1:13 according to the response surface analysis. Under these conditions, the e

10、xtraction yields of chlorogenic acid and flavonoids were 0.81%, 0.51% respectively.2. Development of Eucommia granulesFirstly, optimal additives were screened from different flavoring agents, excipients, and wetting agents. Then preferable method of granulation was selected from three conventional m

11、ethods, namely, fast stirring granulation, wet granulation, rapid mixing granulation, and fluidized spraying granulation. Failure rate of particle size was investigated and then the response surface analysis was used to optimize conditions. The optimal extraction parameters were: 0.78% stevia,-cyclo

12、dextrin to powder: 6, 65% ethanol concentration, ratio of ethanol (L) to the powder volume (kg) 1:6, drying temperature of 70°C, in these conditions, Eucommia granules failure rate of particles size was as low as 6.12%.Based on the results mentioned above, a completed flow of making Eucommia gr

13、anules were proposed as Eucommia leaf after de-dusting, cleaning, and drying 10 min at 105°C, grinding, added 55% ethanol, and the ratio of ethanol (L) to Eucommia leaf (kg) 13:1, and extracted at 63°C about 2.2h, next, concentrated and dried at 60°C. After grinding, the Eucommia leaf

14、 extract powder added 0.78% stevioside, the-cyclodextrin which was 6 times of the weight of other powder, then continued to add 65% ethanol, and concentration of ratio of ethanol (L) to the powder volume (kg) l:6, after mixing, granulated into the machine the rapid mixing granulation, and then dried

15、 under 70°C, 20 minutes.【關(guān)鍵詞】杜仲葉 綠原酸 響應面 提取 造粒 顆粒不合格率【英文關(guān)鍵詞】Eucommia ulmoides leaf chlorogenic acid flavonoids response surface extraction granulation failure rate of particles【目錄】一種新型杜仲沖劑的生產(chǎn)工藝研究摘要5-6ABSTRACT6-7第一章 文獻綜述10-181.1 杜仲簡介101.2 杜仲葉的活性成分10-121.2.1 綠原酸101.2.2 黃酮類化合物10-111.2.3 環(huán)烯醚萜類化合物11

16、1.2.4 杜仲多糖11-121.3 杜仲中的營養(yǎng)成分121.3.1 氨基酸121.3.2 維生素121.3.3 礦物質(zhì)121.4 杜仲葉的保健作用12-151.4.1 調(diào)節(jié)血壓、血脂和血糖作用12-131.4.2 抗氧化，抗衰老及抗腫瘤作用13-141.4.3 增強免疫力141.4.4 減肥美容14-151.5 杜仲葉的開發(fā)研究現(xiàn)狀151.6 藥物劑型的選擇15-181.6.1 固體劑型15-161.6.2 液體制劑16-171.6.3 半固體及其他制劑17-18第二章 杜仲葉有效成分提取工藝18-302.1 材料與方法192.1.1 材料192.1.2 主要試劑192.1.3 主要儀器192.2 試驗方法19-202.2.1 工藝流程192.2.2 綠原酸檢測方法19-202.2.3 總黃酮的檢測方法202.2.4 單因素試驗202.2.5 響應面優(yōu)化202.2.6 數(shù)據(jù)的統(tǒng)計及分析202.2 結(jié)果與分析20-232.2.1 單因素試驗分析20-222.2.2 提取工藝的分析及回歸方程的建立22-232.3 數(shù)據(jù)處理23-292.3.1 回歸方程23-242.3.2