三碘代甲狀腺素鈉鹽作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetLiothyronine sodiumCat. No.: HY-A0070CAS No.: 55-06-1分式: CHINNaO分量: 672.96作靶點(diǎn): Thyroid Hormone Receptor作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 42 mg/mL (62.41 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 1

2、0 mgConcentration制備儲(chǔ)備液1 mM 1.4860 mL 7.4299 mL 14.8597 mL5 mM 0.2972 mL 1.4860 mL 2.9719 mL10 mM 0.1486 mL 0.7430 mL 1.4860 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)內(nèi)使，-20C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)內(nèi)使。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍浮Ｒ韵氯芙獍付颊?qǐng)先按照 In Vit

3、ro 式配制澄清的儲(chǔ)備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄 的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的式助溶1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (3.09 mM); Clear solution此案可獲得 2.08 mg/mL (3.09 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 20.8

4、 mg/mL 的澄 DMSO 儲(chǔ)備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.08 mg/mL (3.09 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 2.08 mg/mL (3.09 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 20.8 mg/mL 的澄 DMSO 儲(chǔ)備液加到 900 L

5、20% 的 SBE-CD 理鹽溶液中，混合均勻。3. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.08 mg/mL (3.09 mM); Clear solution此案可獲得 2.08 mg/mL (3.09 mM，飽和度未知) 的澄 溶液，此案不適于實(shí)驗(yàn)周 期在半個(gè)以上的實(shí)驗(yàn)。以 1 mL 作液為例，取 100 L 20.8 mg/mL 的澄 DMSO 儲(chǔ)備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Liothyronine sodium種有效的甲狀腺激素，可以結(jié)合并激活 1 甲狀腺激素受體 (TR1)。

6、IC & Target TR112體外研究 Liothyronine (T3, 100 nM) stimulates the proliferation of hepatocarcinema cells in which TR1 is overexpressed1.Liothyronine binds to human 1 thyroid hormone receptor (hTR1), and change its conformation. Liothyroninepromotes growth, induces differentiation and regualtes metaboli

7、c effects2.PROTOCOLCell Assay 1 Thyroid hormone depleted (Td) serum is prepared. The growth of hepatocarcinoma cells in methylcellulose isperformed. To determine the effect of Liothyronine (T3) on the growth of cells, cells are plated at a density of 3 104cells/60 mm dish on day 0, and incubated in

8、medium containing 5% regular serum, 5% Td or 5% Td and 100 nM T3.The colony formation in methylcellulose is scored 3 weeks after initial plating1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶(hù)使本產(chǎn)品發(fā)表的科研獻(xiàn) J Nutr Biochem. 2020 Jan.See more customer vali

9、dations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Lin KH, et al. Stimulation of proliferation by 3,3,5-triiodo-L-thyronine in poorly differentiated human hepatocarcinoma cells overexpressing beta 1thyroid hormone receptor. Cancer Lett. 1994 Oct 14;85(2):189-94.2. Bhat MK, et al. Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3,5-triiodo-L-thyronine. Biochem BiophysRes Commun. 1993 Aug 31;195(1):385-92.McePdfHeightPage 2 of 3 www.MedChemECaution: Product has not been fully validated for medical applicat