ML-SA1-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEML-SA1Cat.No.:HY-108462CASNo.:332382-54-4分?式:C??H??N?O?分?量:362.42作?靶點(diǎn):TRPChannel作?通路:MembraneTransporter/IonChannel;NeuronalSignaling儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:20.83mg/mL(57.47mM;ultrasonicandwarmingandheatto65°C)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.7592mL13.7961mL27.5923mL5mM0.5518mL2.7592mL5.5185mL10mM0.2759mL1.3796mL2.7592mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(5.74mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(5.74mM);ClearsolutionBIOLOGICALACTIVITY?物活性ML-SA1作為?種選擇性TRPML激動(dòng)劑，通過促進(jìn)溶酶體的酸化和蛋?酶活性，抑制登格病毒2(DENV2)和寨卡病毒(ZIKV)。ML-SA1抗DENV2RNA和ZIKVRNA的IC50值分別為8.3μM和52.99μM。ML-SA1誘發(fā)?噬。ML-SA1可?于?譜抗病毒研究[1]。IC50&TargetIC50:8.3μM(DENV2)[1].IC50:52.99μM(ZIKV)[1]體外研究ML-SA1(25μM;0~14hours;A549cells)possiblyaffectstheentryofDENV2intohostcells[1].ML-SA1(0~200μM;A549cells)showsthatthereisnocytotoxicitytothecelllineobserved,evenatconcentrationsupto200μM.ML-SA1(0~50μM;A549cells)significantlysuppressesDENV2attheRNAlevelsandtheIC50is8.93μM[1].ML-SA1resultsinadose-dependentdecreaseinZIKVinA549cellsatboththeRNAandproteinlevels,andtheIC50valueofML-SA1againstZIKVRNAis52.99μM.ML-SA1,asanactivatorofTRPMLs,appearstobeapotentinhibitorofDENV2andZIKVinvitro.ML-SA1promoteslysosomalacidificationandproteaseactivitytoinhibitviralinfection.ML-SA1caninduceautophagyinHuh7cellsorA549cells[1].WesternBlotAnalysis[1]CellLine:A549cellsConcentration:25μMIncubationTime:0~14hoursResult:PossiblyaffectedtheentryofDENV2intohostcells.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?Autophagy.2022Sep10;1-16.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].XiaZ,etal.ML-SA1,aselectiveTRPMLagonist,inhibitsDENV2andZIKVbypromotinglysosomalacidificationandproteaseactivity.AntiviralRes.2020;182:104922.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.