SYP-5-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemESYP-5Cat.No.:HY-100693CASNo.:1384268-04-5分?式:C??H??O?S分?量:312.38作?靶點:HIF/HIFProlyl-Hydroxylase作?通路:MetabolicEnzyme/Protease儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:33.33mg/mL(106.70mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.2012mL16.0061mL32.0123mL5mM0.6402mL3.2012mL6.4025mL10mM0.3201mL1.6006mL3.2012mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(8.00mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(8.00mM);ClearsolutionBIOLOGICALACTIVITY?物活性SYP-5?種新型HIF-1抑制劑，抑制腫瘤細(xì)胞侵襲和?管?成。IC50&TargetHIF[1]體外研究SYP-5inhibitshypoxia-inducedupregulationofHIF-1.SYP-5inhibitsHIF-1anddownstreamgeneexpressioninHep3BandBcap37cells.SYP-5inhibitstumorcellmigrationandinvasion,aswellastumorangiogenesis,whicharemediatedbysuppressingPI3K/AKT-andMAPK/ERK-dependentHIF-1pathway.Theproteinsofvascularendothelialgrowthfactor(VEGF)andmatrixmetalloproteinases(MMP)-2thataretargetsofHIF-1,aredown-regulatedbySYP-5.SYP-5displayssignificantinhibitiononhypoxia-inducedoverexpressionofVEGFandMMP2inbothcelllines.Inthetubeformationassay,SYP-5suppressesangiogenesisinducedbyhypoxiaandVEGFinvitro.SYP-5alsoretardstheHep3BandBcap37cellsmigrationandinvasioninducedbyhypoxiaandFBS.SYP-5specificallyinhibitshypoxicinductionofluciferaseexpressioninU251-HREbutnotinU251-pGL3[1].PROTOCOLCellAssay[1]Thecells(1×105cells/mL)areseededinto96-wellcultureplates.Afterovernightncubation,thecellsaretreatedwithvariousconcentrationsofSYP-5(2,10,50μM)for24h.Then10μLMTT)solution(2.5mg/mLinPBS)isaddedtoeachwell,andtheplatesareincubatedforadditional4hat37°C.Aftercentrifugation(2500rpm,10min),themediumcontainingMTTisaspirated,and100μLDMSOisadded.Theopticaldensityofeachwellismeasuredat570nmwithaSpectraMaxParadigmReader[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻?CNSNeurosciTher.2022Jul20.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].WangLH,etal.SYP-5,anovelHIF-1inhibitor,suppressestumorcellsinvasionandangiogenesis.EurJPharmacol.2016Nov15;791:560-568.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedi