醫(yī)用生物信息基礎(chǔ)參考

1、實驗名稱：肝細胞核因子1a(HNF1a)基因的生物信息學研究姓名：班級：學號：實驗?zāi)康模?.掌握中國期刊網(wǎng)全文數(shù)據(jù)庫中原始全文文獻的搜索及獲得方法.2 .熟悉生物信息學常用網(wǎng)站中的全文或文摘的獲得方法.3 .熟悉網(wǎng)絡(luò)搜索工具,瀏覽器及生物信息中央,掌握醫(yī)學信息獲取途徑.4 .掌握PCR引物的設(shè)計原那么.學習PCR引物設(shè)計軟件使用方法,設(shè)計基因的引物.5 .了解核酸序列數(shù)據(jù)生成和常用分析工具；了解序列比對的根本原理,熟悉常見的Blast程序.6 .了解蛋白質(zhì)分析的根本內(nèi)容,熟悉常見的蛋白質(zhì)分析序列；進行蛋白質(zhì)物理性質(zhì)的預(yù)測和二級結(jié)構(gòu)和折疊預(yù)測的在線操作.實驗方法和流程：一、選擇研究基因選定感興趣

2、的基因：在中國期刊網(wǎng)查詢與藥學有關(guān)的基因,查得依地福新是日前所發(fā)現(xiàn)的第一個通過激活細胞內(nèi)Fas/CD95死亡受體而起作用的抗癌藥物.Fas是近年來研究得最為深入的有關(guān)細胞凋亡的膜外表分子,說明它們在凋亡中的作用機制,對深入了解細胞凋亡的機制有重要的意義.登陸西安交通大學主頁(://)點擊“圖書館點擊“進入圖書館“查找資料欄下,點擊“網(wǎng)絡(luò)數(shù)據(jù)庫導航進入“網(wǎng)絡(luò)數(shù)據(jù)庫,點擊中文數(shù)據(jù)庫中國期刊全文數(shù)據(jù)庫CJFD(中國期刊網(wǎng))-CNKI點擊登錄,進入全文數(shù)據(jù)庫選擇檢索途徑：初級,選擇學科范圍：勾選“醫(yī)藥衛(wèi)生檢索項選擇“關(guān)鍵詞,檢索詞輸入框鍵入“青少年糖尿病基因,時間默認,點擊“檢索匕皿泊

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6、醇啜點花束時三十鼻皆舊.土詆H芋以1巾綠音題iWtmrMMki立由E如iCtBM蚣崎：由里*0昆尸例由呢妒0£：£,事正寄江擊艮1k刖片嶺看中匕歪一退M.K.后詫清仃口二呼FBfifMIHEM登門能K¥=2用#*F"S%哼-牙麗E用EL?在PUBMED中進行外文文獻的檢索Pub%&d.rU.邕N*MW-tiF-ryUMrdcrfIdorlE玉ristrJie?©1rfesar導HWrf|pu匕MeEMODt3QRSSGsesearefiLimSwftsnceclssarthHe?StcrdiL也隋丁-自二.時:£|UunvHu

7、w曲門?點口也s5itea配Rf-:ei-4yMdd£3Results：1t.20of196口Clinical如口ll詣innofbestprattlCEguideWiesfiorHiegenetitdiagn口乖ofM8Y口ndM00Y3.1 -IncaniM«CambuliVW,CsvalctF,CcnjiLiT.PaderiM,SentineliF,RtuneaS,PnyP.SoroM,PiliaS.LecheS.Co口冶HR:切燈?01nNotf27(11)1331-3.tioI1C111v?口1口口卻95富N.i網(wǎng)的£fag|lWFP隔D;200384P

8、ubNed-inprocemNRB3t£(I?lam號匚t1發(fā)2口n3巴ivciTniter.42林於士,世廣n午id_acei31七iirnn,人川亡中廿北獷"七巾巳飛2 -Hiang.1_"如勺LLRhoad?DB.EJI41tchnDI.2UlLJCt15；150(2259-5/.bpjD2DIUicp17.PWO20ftij(M86PjIHed-rpijcf5sH-凱£(ctaton方ricihlFhFlEEiygmi弓nnj%tinn0iiMulvingbothHMFtAAJiriCY甘刁nilHHF4內(nèi)'MCTWI1印p虧ai習qpi

9、rprirtForlaniG.zuccros.DiRoccoA,dimaoR,scipion?M,MarascoE,rdhsog,MarcnesiiniG,Mantavaniv,L)i曲era©Caa201Cm.片美(11/？金亞9.bpuD201Ajg12.PMID30705777PUbMRd-lrptOCBSS利用生物信息數(shù)據(jù)庫獲取基因序列:翻開NCBI()網(wǎng)站,點擊左邊的下拉條選擇"Gene",輸入“HNF1a顯示如下結(jié)果：4：HHF1AOfficialSymbolHNF1AandName;HNF1homeaboxAHomos

10、apiensOtherAliases:HNF1,UFE1,WODYS.TCFIOtherDesignations:Interferonproductionregulatorfactor(HNF1);albuminproximalfactor;hepaticnuclearfactor1;maturityonsetdiabetesoftheyoung3;tran5匚iptiunfactor1.hepatic;transcriptionfactor1,hepatic;LF-B1.hepaticnuclearfactor(HNF1),albuminproximalfactorChromosome:12

11、;Location:12q24.2Annotation:Chromosome12.NC_000012.10(119000932.119924698)MIM:14241DG«nelD:6927點擊紅色有下劃線的“HNF1a,可獲得此基因的根本信息,如基因的名稱,全名,基因來源物種,基因的其他名稱,如下列圖所示(見下頁)：OrdercDNAcicne,Links1;HNF1AHNF1homeoboxAHomosapiensWenI口：15g27SummaryOH'icialSymbolHNF1AprovidedbHGNCOfficialFullNameHNF1homeoboxAp

12、rovidedbyHGM/PrimarysourceHGNC;11621SeerelatedEn-mbl：EN5G(CIQJQ1351QCI:HPR匚:口口3口0；MIM:1424LQGenetypeproteincodingRefSeqstatusVALIDATEDOrganism也m.總總pi§ns_LineageEukaryota;Metazoa;Chordata;Cr&niata;Vertebral;Suteleostomi;Mammalia;Eutheria;fuarchontoglires;Primates;J-/aplorrhini;Cctsrrhini;Hom

13、inid3e;HomoAlsoknowna5HNF1;LFB1;TCF1;MODY3;HNFlftGenomicregions,transcripts,andproduetsGoTor日口匚日s日du日nee日日milsTryour型蝌號®uliwriceViewerNC_00Ml2.11402|kH妁之嶼岫bi43、她L3吟&HBHi1=M即口聊5訴*4B曉船-cin$i|-6tmml儀kd點擊左側(cè)藍色的nm000545.4llinks“Genebank可獲得基因NHF1a的堿基序列,該基因的CDS為“24.1919,該基因的NP號為“NP000536,堿基序列如下列圖見下

14、頁：61121131241?013614Z14B1S4101661T71cgtggccctgtggcggccctcggcrccctagtcgaggggaagacgiacgagccctgagaggcgtgtg3ceggtegataaccatgaagactggegesgeaagctaccaactcctcftt.cca1-rrrr-nrmerf"rtggcagccgagetegagteacctcctggctgetggetgagcgatggggaaggcgorccGacgaagatggteactggctegcagaagcggrgttcacccatcaagaaggggggcctatgagr1n

15、n十BhCEirfLrrgccratggtctgggetgagesggagaaggeectgcccaatggacttcacgccagraaagccgaagtcctacc國acc昌gteccgccgccctgtgcagggcaggcggaggaaccaggcagaagaPf-a-ShFELn!"LletaaactgagaagaggcactCcctgacaaggctgggggacacccatccttgtggagactqu目守c;曰qc白acctgtcccaacacctggtagagggetgatgcttcaagcgaccetagcaascamrrrrrrrTl"ccagctge

16、aggatccaggcagggagtccgactcggggccaaagagetgcctctgcacaacatccrcaacacctcaaccgtccgcaagtgaagagcccciggcccagcaggagageganr!-llllqI-Lnacggagctccctgggtgagctgcggcggcgtccgaggacggagaacctcagaggacccgtcagegggaggaagggcactccagcgagaggacaggtgatgtcccagcagagagaegetaglnerl01rMarlf在NCBI中進行Blast比對復制第二步中所得的堿基序列,從NCBI首頁點擊Blast,可

17、出現(xiàn)下邊圖示的頁面:BasicBLASTChooseaBLASTprogramtorun.mi-cleotideIastSearchanucleotidedatabaseusinganuckotidequeryAfgorjthms:Llastn,megablast,discontiguousmegablastprof電LI門allhlleTXSearchproteindatabaseusingaprol&inqueryAlgorithms:blastp,psi-blast,phi-blastSearchpiotdndatabaseusingatransLiteiinucleoiideq

18、uerySearchiMiislatediiudeothkdatabaseusingapiolehiquerySearchnan&laiediiucleetidedatabaseiiisingatr«insLiteilnucleoiid&query點擊“nucleotide,可出現(xiàn)下列圖所示頁面,在圖示的大框中粘貼堿基序列,按如下所示選擇各工程,然后點擊“blast,可出現(xiàn)比對結(jié)果下第二幅圖和第三幅圖BLASTBastelocsiAiignrMntSearchToolHomeRecentRmuItsSavedStiategiesHelppBIfBLASI/biastn

19、siwc.FormattingResultsF)K2NO»WIO1REd*andRMubtnHSaveSearchSlrateQiesiFwmatEarotiorw»DjwnioadNucleotideSequence(3241letters)QueryIDDfs<riptionMoIpcuIptyppQueryLengthlcl|10779Nonenucleicncid3241DdtcihflseN(im眄ndex/9606/aIIcontig_dnd.rnaDescriptionHumanbuild36.3RNA,rafarwncs?andasssmbli-Pro

20、gram2LASTN22.19421WonOtherreports:>SearchSunrrtryITaxnnnrrYrc,ortrZi,tancctreeofrowlfa：fHumancoomcYic/dGraphicSummaryDistributionof19BlastHitsontheQuerySequenceMousaovertosapih.dofliipclicktoshowslgnmtnisqAlWnnientr|s且sfl昵匚.545.4ILength=3241HomosapiensHNF1hosieoboxA(ENFLA)fmRNAsetAll3譏sl-Gtmd8,uo

21、g(jqDiMmncQtrmc0fmsultuENEID:四£7HHE1Al|IfllElhcmEcboKAEdwdnupi匚g(Ovex100FubMcdld.wk»)Score=55S6bits(321)rEzpect=0.0Identities=221/3241(10D4J.心安封=O；3241費Strfind-Plus/F±usQueryShjnt11二GIGg：CCIGTJGCA&C：GA&CCATGG7TTCTAAA二TGAGCCAGC7i>CAGACUGAGCICClllllllllHlllllllllllllIIIIIIIII

22、IlliHUHHUIIIIIIGTUG'CtTGT'GC也GC:GR3TGG"TTCTAAaUTGAGC:、AGC7GCAGACGGAGTC.C60finQueryCl1G&CGjCCCT03TCfiAGTCAGCG2TGAGCAAAGAGSCACTGATCCAOGOACTOCGT3AGC1111111111111111111111II1111II1111II11111111111111111I1I111I120SfoCt61IGjCGjCCCTGJICGAGLCAl?GG:TGAGCAAAGJLGCACTGATCCAGGCACTGGO!GkGC12UOury

23、121CG4GGCCCTAC7TCCTGjCTBGAm艮&G4C：CCCTfACAA霍G盛又GTCCTGCGGCGGCG11111111111111111111111111111111111111111111II111111111111111ien121C<5(jCG：GCTACCTCCTGjCTCCA3AA；GCZCCUHbWACAA仔WGSRSCCT68.C(5dCG100Query191GTCGA三GGG工G;TG&TSAGCTGZCCAATSGGCTGGGGGAGACTCGGGGCTCCGAGGACG1111111111111111IIII11IIII11IIII

24、11IIII11IIII1111111111111111Z4USbjtit191GTCCA二色白白上仁:T仁榮C亡LAT=母紀T自公至工！L匕ACBTCGCGCTC.CGAGkCCr240Query241A3AC05AC住M3AT9GG3AAACTTCk<依CACCCATCCTCJULA'SAGC!T(3OAGAACCTCA11111111111111111111111111111111111111111111II111111111111111JOOSbjct241AGACGjACSACiJATSGGjAA&ACTTCAC笑CACCC4TCCTCAAAGAGC7GGAGA

25、ACCTCA300Query1GCCCTF&UG工GiCGGCCTACPiGiRCTGTlGirC2rTTTOTAGdGGACCCGT閂白門四、設(shè)計引物選定需要PCR擴增的目的序列,進行引物設(shè)計,本實驗中所選基因HNF1a與MODY3有關(guān),查閱文獻可知該基因2號外顯子上游白內(nèi)含子由G-A置換與MODY3有密切的關(guān)系,故為了深入研究,針對其二號外顯子進行引物設(shè)計.在NCBI中選擇“gene后可查得有關(guān)信息,如下列圖：350.54g/白2n=MHNFl我m/gene_synaiiyro=ftHTJF1p,/geiue_synonyni=rrLFElr,/genc_synaziyiti=PF

26、TCF1p,7gene_3yncnym=f,MOBY3"/inference=Praligimeiit:StlignPl/nujuber=2在google網(wǎng)頁中搜索“primer3",點擊第一條搜索結(jié)果,可出現(xiàn)下列圖所示網(wǎng)頁,在最上面的對話框中粘貼HNF1a的堿基序列,按下列圖所示填寫"targets欄和"ProducesiteRages"欄：geccjijaagag;ajaas2clzc2?cij:j?LffcaaaaQGau二3七叵Pirjrmprimer.QrwcJcftpnmerbugw;?記“也力任由川方由probe團:5聞?pCTU

27、M：lig?bcltiwEPir<rihtprrw£r,紂ysegjitp出口亡hH4宣(5,to31.qoppositertraudl：,11k121191241tggeggeect.七pagncgrjcga.tggcfagccE'ayztcJ=HJlLL：aLceccocci0&xcigfEitmmuxiagcaE.ggtttggyctgsugQBL丁仃*口0.0口0七匕RB00匕丁mwttuzsug1匚zugaguctiLl30430acilREMGUd爐口匚匚匚陽£cct=ea.gEt產(chǎn)a,jn緲qqaar.修inetcdwxg-tgBcgca

28、gr=ticccrigcgctGcgrQ'cjacgtecffngijgngetnr=匕匚百入-IPickIPrmeis|ReseiForm£*加u修Id."|A內(nèi)聯(lián)gideuiiyourogutT-v.-mF-jm艮旦5Ub2TsqijsjpiajjciyI.s-jcr-jun-dLe2bwe.城p中加oue二力皿$51.OiinuikUit盯皿行皿匕聞此sd-.A7CTCCCC1TCAT,皿在EMplzjjwgluuj：0迅山e匕心源CCCC.EHdudtedIEgWQl.8,3Ebrbjdsselect!sn學ffTmer；?inShe7basesotartd

29、ngat401andibe3basesat68Ojmurkihe；3口甄四F但1s加9&(Whte勵thvand=:電g.ATCT<CCCCTCAT.fbrtMdspTtmerstndi?cefitrHC8C居匚3年及snog上XOMDHtim乩:rT©豆Mura5|M】iab曲yB0LlwJUer基工也£皿江以12CCPaMaaKerEl出不爾Hie24COtaxTehipIbte2疝“叩12LtPjiir口忸血業(yè)MLsrimYanaLOP»ckFrmeiisReeeIFnrmi填寫好各欄數(shù)據(jù)后,點擊“PickPrimers",可得如下結(jié)

30、果,下列圖為首選引物:OLIGOlen即看emy_31LEFTPRIMER2742059.945S.004.002.00ccacctcaaagagc七ggagRIGHTPRIMER6022060.2655.DO4.00C.00ct;catcacctg-trgggctcttSEQUENCESIZE：3243INCLUDEDREG工ONSIZE;3243PRODUCTSI2E:929,PAIRANYCOMPL：tJ.OO.PAIR31COUPLi3.ODTAP.GETS(Start,len):3£0f2001CQCffgccctytQgcaffccffaffccatffutttctaaa

31、ctsaccayctjcaffacrsgacctcc61tggcggccctgctcgatcagggccgagcaaagaggcactgatccggcactgggrgage121cgggTgccctacctcctggctggagaaggccccctggacaaggrgggagtcctcjcggcggcg181gtcgagggagctggccgagcc.geccaat.gggctgggagacrcgggttcicgaggacg241agacggacgacgatggaagactt-cacgccacccaccrctcaaagagct-agagaacctca>»>»&#

32、187;»>»»>»»301getc寸總曰口ag)gcgcccaocagaaaecgtggtgacracectter.qca白白白目目acecgt361ggeggtggcgaagat燈目匚caatcctacc：tgcagcageacaaca'CcacaGagcgggag(g+t士方+%由F才+*F古青*興*tF前卡雷需至十*+*K*啟*fF*1f*需十才*才土*需宣*421tggteataccactegect.caaceagteceacccgECCCaaeaccrcQaeaaggcaccc重*言高*卡*去意*畬£卡

33、古力去上全土舍高*弋*味金*弋*卡金龍肅黃去擊言去塞左W*京大F#前云前病青土*木塞雷士*卜圖為備選引物KEYStinorderciprecedence):target.»»»leltprtwer<<<<<<rightprimerADDITIONALOLIGOS更p"t_甄m.any3,'1LEFTPR工HER2742059、9455-004皿2.00ccatcctcaaagagctggagRIGHTPRIMER60E2059.7055.004,002.00tggtagetcateacretgtggPRODUC

34、TSIZE:335.PAIRANYCOHPL15.on,PAIR31COMPL:2.002LEFTPRIMER27420S9.9455.004.002.00ccatcctrcaaagagctrggagRIGHTPRIMER6682060.6255.004.000,00gaacaggatetgetgggatgPRODUCTSIZE：395rPAIRANTCOMPL：5.OC,PAIR31COHPL：2皿3LEFTPRIMER2742059.9455-004+002.00ccatcccaaaga9ctggagRIGHTPRIMER59620M6650-003,001.00acctgtgggctct

35、tceatcaPRODUCTSIZE:323,PAIRANTCOMPL!6.00,PAIR31COMPL:1.004LEFTPRIMER2742059.9455.004.002.00ccatCctcaaagagctggagRIGHTPRIMER5972060.6655.004.00cacct.gtgggctcttcaatcPRODUCTSIZE:324fPAIRANYCOHPL:6,皿PAIR31CQMPL;2.00Statisticscontooininnotmtinhighhighhighsidmanytar£JXCltoadGCtoocooany31polyenderedNsg

36、etregGC%clamplowhighcornplcomplXstabokLeft.29320D094n5i249055040207Right22245110569014901731903504362317PairStats:considE匚曰(13118,unacceptableproduct-size3口95,highendcomplok22prixnerSrelease1.1,0五、蛋白質(zhì)分析在第二步所得頁面中點擊NP000536,可得基因HNF1a所編碼的蛋白質(zhì)序列,如下列圖所ORIGIN16112L1612130136142148LSL6DL/irtvsklslqtpnglq&am

37、p;trgssy.lqqtiilipqciqrgvspsqahssplpppallsteaklv31gptftrtgqshvtqspfniqvfissdteassdssngqshlsllaallesedatdddgedrewcittglngdeIptkltgraqglganlvtalspsRvhgvsaaggplppvostIviglasatmaeg1qspha亨gLhtp國nIpsnhsvietLslcealigLftpptlkeleqshJsqhliikiEiirfKwcjpasevrvynwfan3etstItalhslerqQqsvpvinalyslikpeva£isvqni

38、asssgegpyliagnlepeaaahqcitpmktqkr&cjqiL工qayerrtkeeairhktevpsssggpqtspglnqqp3ftl0SSltlqegyt-htgllpcdpasiqtilqpegpldkgesckawetllqealytwyvrkciqknpmKuu匚匕l(fā)amdtysgppIvtvstplhqqnliinaslpgpvqfsqplhptmlitdttnl京htgggrgu1尊匕LdpurvatattvfcrefvaqqftliatlveecucaepgpgpgpaJpvsptgl&psbvrutigpgepasyqgplmppvsal

39、asltprki35S3ivlyqs利用ProtParam(expasy.ch/tools/protparam.html)查詢蛋白質(zhì)根本性質(zhì)如下列圖:Molecularweight:67386.4Theoreticalpl:5.82Aminoacidcomposition:CSVformatAla(A)487.6%Arg(R)203.2%Asn(N)182.9%Asp(D)142.2%Cys(C)30.5%Gin(Q)507.9%Glu(E)436.8%Gly(G)487.6%His(H)223.5%He(I)142.2%Leu(L)7111.3%Lys(K)223.5%Met(M)121.9

40、%Phe(F)111.7%Pro(P)609.5%Ser(S)7311.6%Thr(T)518.1%Trp(W)40.6%Tyr(Y)121.9%Vai(V)355.5%Pyl(0)00.0%Sec(U)00.0%(B)00.0%00.0%(X)00.0%Totalnumboroinogatlvolyonarqodroslduos(Asp4G±u):57Totalnumberofpositivelychargedresidues(Arg十Lys):42Atoniccoitposition:CarbonC2945HydrogenH4b85UrtrogenU829Oxygen0950Su

41、lfursIS上OFJlula：.29"看685"8291950,15Totalniunberofatcwis:9424Extinctioncoefficients:Extinctioncoefficientsareinunitsofcm-1/at280nm£xt.coofficiontAbs0.(W1g/1)400050.594/aoovmingALLCysresiduesappearmeasuredinv/ater.shalfcystinesEirt.coofticiontAbs0.1%31g/1)halfcystines398800.592/assuming

42、WOCysresiduesappearEsl.ihcilf-lifejTheM-terminalof二h.ocq'jcncoccnsi4credisM(Het).Thhalf-llf*ij:Lnvitg).30huu工守A.itinHliaQr4LiaiiLoeytar>2Dhours(yojcrtfinvavo)->IDJiours(Eschirichi*colinvivo).lustabilityiudex:Theinetabilityindex：1工JiscoxputdcoN?59.32uhinclassifiestheproteinanunstable.Alipha

43、ticIndex：JS.23sandEcmg電ofhydropathiclty(qeavy)：-0,452禾ij用TMPred(://cgi-bin/TMPRED_form_parser)預(yù)測蛋白質(zhì)跨膜序列和方向,結(jié)果如下列圖：LPossibletransmembranehelicesThesequencepositioiisinbracittsdenonunate仕itcoreregion>riy；.017：彳aboirOCarecotiRidfitfid百即ifkart£rowtoacoreeenter403(4031423(423|7S3413i

44、22(IZ8415(41L|848133SZL(SZ1515(S13|29533Outsideginaidehelicea:2foundCrowtoscorecenter389(359|363(38川390379130(q39(S33I606130Insi.deldo'utoideheLloco;3found2.)T:ibleufcorrespondencesHere而showilywhichcfthemade->ci.tsi'ieheJeescorr«sp-3ndXwlxchth=cUride->inrklehelicesTftictEshevinbra

45、cketsarecn?idtredininn:antA"sj'mLfrJinlicafssapreferenceofHus©tit抗aEotuA,LH-"-symbDiidratesastrongprEferencEofthisorientation.inside->outside|403-423打753422-44s(2S|648521-545(251294-+4-+I(IIIIoutside->inside359-389(21|390i420-439(201£883JmcnklsliirIranKmenibranelopoloIj

46、lcecsriggTEb.viisarepme-REpscijJatvr出dzhjiuldbrik=dwithexireiiiecaution>in_etheyar-basrdoath=口口tliaia_iramsmeotiranehrjcr£havebiEiifouiiii.M.rtK拗iBiseziieCccrrxinifTiceTiblesbowiafc-oi-e討theptt-ictimiplol在雄廳atjreadshouldbeusedforUeto里口】ugy郭鄉(xiāng)穹mlentolutAjj".rtHb®玲El*enicseir|?i5i#onl

47、ycigmificwtumeE->sllQjjclpifcfeuedmdels：NreriftinusinsideIrxanairemrtr.»ietie1ices-rtotalfltccs:753#tcleogth.jiceizeorlentatieci1403311L|7531-G->aLteczk.3.t,iv-rrcdel1stratigt*BLasjrEJurt：aneneJiceSa.total白亡0e史:&3Urrsftifceleth.steereariencatin14£口蟲坦|20>)|EB目0-1THpr*daulpuk也？u

48、nknnkMi1CW-32C0-4MA-8皿-tmu.利用SignslP3.0(:cbs.dtu.dk/cgi-bin/webface?jobid=signalp,4969A19001B46C57&opt=wait)預(yù)測信號肽位置和結(jié)構(gòu),結(jié)果如下列圖：signaJLPINNresult:Sign巴PINNprodic-tion(俗ukne-uorks?“Sequencevsfoqumncm*MeasureixCmaxYmsxSmeanSDposition2323131221122ImngchH70ValuecucoHIf2772787083993390000sign-lp工MMprdioeon?,uksiutn;hl,8ionpjob3VSKLSQLQTELLAALLESGLSKEALIQX>LGEPGPYLLAGEGPLDKGESCGGGRGEL1>ELPNGLGEa曰曰3n曰huePrediction:Non-secreDoryproteinSigna