Gallic-acid-3-4-5-Trihydroxybenzoic-acid-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEGallic acidCat. No.: HY-N0523CAS No.: 149-91-7Synonyms: 3,4,5-Trihydroxybenzoic acid分式: CHO分量: 170.12作靶點(diǎn): COX; Reactive Oxygen Species作通路: Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B儲(chǔ)存式: Powder -20C 3 years4C 2 year

2、sIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (587.82 mM; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 5.8782 mL 29.3910 mL 58.7820 mL5 mM 1.1756 mL 5.8782 mL 11.7564 mL10 mM 0.5878 mL 2.9391 mL 5.8782 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存式和期限。

3、BIOLOGICAL ACTIVITY物活性 Gallic acid是COX-2 的抑制劑。IC50 & Target COX-2體外研究Gallic acid is an antioxidant which can inhibit both COX-2 1. After 18h treatment with Gallic acid, thenumber of viable neutrophils is dramatically decreased from 40.3% to 27.7%, highly comparable with 26.4%1/2 Master of Small Mole

4、cules 您邊的抑制劑師www.MedChemEfor untreated neutrophils. Gallic acid fails to attenuate isoproterenol-induced myocytolysis 3.體內(nèi)研究 The food intake (2.60.08 g/day, p=0.69) and the body weight (2.50.69 g, p=0.76) of the Gallic acid groupdo not differ significantly from those of the control group (food intak

5、e; 2.410.14 g/day and the body weight;2.830.84 g/day). The blood glucose tolerance in the Gallic acid group is significantly improved after 2 weeksof treatment. The blood glucose tolerance of the Gallic acid group after a treatment period of 2 weeks is alsosignificantly better than that of the contr

6、ol group at 90 and 120 min ( p 2.PROTOCOLCell Assay 3 Neutrophils are treated with 8g/mL Gallic acid in RPMI1640/10% FBS for 3, 6, 9, and 18h. At the end ofGallic acid treatment, the cells are stained with Annexin V-FITC and PI according to manufacturersinstructions. Briefly, the cells are washed tw

7、ice with ice-cold PBS and resuspended in 1 Binding Buffer at aconcentration of 1106 cells/mL. Cell suspensions (1105 cells in 100L) are incubated with 5L of AnnexinV-FITC and 10L PI in a 5mL culture tube at room temperature for 20min. The stained cells are immediatelyanalyzed on flow cytometry syste

8、m 3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Five-week-old male C57BL/6 mice are used in this study. The animals are maintained in a temperature-Administration 2 controlled room at 22 C on a 12 h light-dark photocycle. The mice are divided

9、 into the control vehicle groupand the Gallic acid group. For 2 weeks, the mice are administered intraperitoneal treatment on a daily basiswith vehicle (10% alcohol, 10% tween 80, and 80% saline) alone or with 10 mg/kg Gallic acid. After thistreatment, GTTs are again conducted, and the blood samples

10、 are taken for subsequent biochemical analysis.Over the experimental period, food intake and body weight are measured on a daily basis 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Amaravani M, et al. COX-2 structural analysis and docki

11、ng studies with gallic acid structural analogues. Springerplus. 2012 Dec;1(1):58.2. Bak EJ, et al. Gallic acid improves glucose tolerance and triglyceride concentration in diet-induced obesity mice. Scand J Clin LabInvest. 2013 Dec;73(8):607-14.3. Cheng Y, et al. Plant Natural Products Calycosin and Gallic Acid Synergistically Attenuate Neutrophil Infiltration and Subsequent Injuryin Isoproterenol-Induced Myocardial Infarction: A Possible Role for Leukotriene B4 12-Hydroxydehydrogenase? Oxid Med Cell Longev.2015;2015:434052.McePdfHeightCaution: Prod